Browsing by Author "Tavmergen, E"

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    Differentation of human spermatogenetic stem cells from azospermia patients to make sperm-like cells
    Gozuacik, D; Vatansever, HS; Kara, B; Calimlioglu, N; Yasar, P; Tavmergen, E; Goker, ET; Semerci, B; Baka, M; Ozbilgin, K
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    COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm
    Durmaz, A; Miçili, SC; Vatansever, S; Gündüz, C; Bagriyanik, HA; Dikmen, N; Göker, ENT; Tavmergen, E
    Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. An accurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It is supposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these types of tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. The infertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examined the ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNA damage and embryo quality.
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    Premature luteinization defined as progesterone estradiol ratio >1 on hCG administration day seems to adversely affect clinical outcome in long gonadotropin-releasing hormone agonist cycles
    Özçakir, HT; Levi, R; Tavmergen, E; Göker, ENT
    Aim: To examine the effect of premature luteinization on the outcomes in long gonadotropin-releasing hormone agonist cycles. Methods: Two-hundred and forty-eight patients who had undergone assisted reproductive technology for infertility treatment between 2001 and 2002 were enrolled into the study. The patients were separated into two groups according to P/E2 ratios on human chorionic gonadotropin administration day. Group A consisted of the patients whose P/E2 ratio was 1 (n = 116) and Group B consisted of the patients with premature luteinization of which P/E2 ratio was > 1 (n = 132). The P/E2 ratio calculation was performed as follows: P (in ng/ mL) xdagger1,000/E2 (in pg/mL). The primary outcome measures included oocyte quality, fertilization rates and clinical pregnancy rates. Results: The mean number of mature oocytes retrieved in the groups were 9.5 +/- 4.8 and 6.4 +/- 3.6, respectively, and the difference was statistically significant (P < 0.05). Although the difference between the fertilization rates in Group A and Group B was not statistically significant (P > 0.05), the clinical pregnancy rates seemed to be affected adversely in the Group B patients with premature luteinization (41.4% versus 28%, respectively; P < 0.05). Conclusion: Premature luteinization, defined as P/E2 > I on human chorionic gonadotropin administration day, in long gonadotropin-releasing hormone agonist cycles seems to adversely affect clinical outcome.