Browsing by Author "Tepe H.D."

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    Investigation of the changes in low molecular weight organic acids and other physiological parameters by nitric oxide application in two wheat cultivars exposed to boron stress
    (Taylor and Francis Inc., 2017) Tepe H.D.; Aydemir T.
    Boron (B) is one of the essential nutrients for the growth of plants, but its high concentrations are toxic for plants. Thus, B toxicity is a big challenge in crop cultivation. Nitric oxide (NO) is a small signaling molecule that has cytoprotective roles in plants. We investigated whether exogenous sodium nitroprusside (SNP), which is a NO donor, may succeed to alleviate B-induced toxicity in wheat cultivars. Seedlings were grown for 10 days in a growth chamber at 25°C with 16 hr light–8 hr dark photo cycle. After high B application, the effects of SNP on growth parameters; electrolyte leakage (EL); changes in reactive oxygen species [contents of hydrogen peroxide (H2O2), malondialdehyde (MDA), and proline]; the activities of antioxidant enzymes [glutathione peroxidase (GSHPx), glutathione reductase (GR), and glutathione S-transferase (GST)] and nitrate reductase (NR); and low molecular weight organic acid (LMWOAs) contents and also chlorophyll and total carotenoid contents were investigated in both shoots and roots of two different wheat cultivars. All experiments were carried out in triplicate. 0.2 mM SNP application ameliorated the chlorophyll and total carotenoid contents, and growth parameters such as shoot length, root length, and fresh weight in both wheat cultivars exposed to B stress. SNP reduced the B-induced lipid peroxidation, EL, and proline and H2O2 content in wheat cultivars. SNP application also increased the activities of NR and antioxidant enzymes, including GSHPx, GR, and GST in wheat cultivars exposed to B toxicity. All of the tested LMWOAs including succinic, propionic, butyric, oxalic, formic, malic, malonic, and benzoic acids were increased by SNP treatment in the shoots and roots of both wheat cultivars exposed to B toxicity. In conclusion, results obtained from this study have demonstrated that interactive effects of SNP with B considerably reduced the toxic effects of B in wheat cultivars. © 2017 Taylor & Francis Group, LLC.
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    LC–MS/MS analysis, antioxidant and anticancer effects of phenolic-rich extracts from Algerian propolis: a comparative study
    (Springer, 2023) Boulechfar S.; Akbulut Z.; Tepe H.D.; Zellagui A.; Aktas R.G.; Bensouici C.; Doyuk F.; Khattabi L.; Demirel G.; Lahouel M.
    The present study was aimed to compare the phenolic composition and the functional properties (antioxidant and anticancer) of phenolic-rich extracts of two different propolis collected from Collo (PREPC) and Oum El Bouaghi (PREPO) regions. The phenolic composition was determined using LC/MS–MS. The antioxidant activity of the extracts was evaluated using alkaline DMSO and β-carotene-linoleic acid tests. Anticancer effect on HepG2 human hepatocellular carcinoma cells was determined using CCK-8 assay. Twenty-two phenolic compounds in PREPC and twenty-three in PREPO were detected and quantified by LC–MS/MS. Ferulic and caffeic acids were found to be the predominant compounds. Both extracts were able to inhibit lipid peroxidation and demonstrated their ability to scavenge superoxide radicals more effectively than the standards. The highest lipid peroxidation inhibition (IC50 = 17.58 ± 1.98 μg/mL) and superoxide radical scavenging effects (IC50 = 6.19 ± 0.24 μg/mL) were exhibited by PREPO. However, PREPC showed stronger cytotoxic activity against HepG2 (IC50 = 12.22 ± 0.05 µg/mL) than PREPO (IC50 = 18.68 ± 0.33 µg/mL). These results demonstrate the potential of extracts from Algerian propolis to be used in functional formulations. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.