Browsing by Author "Tlili, N"

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    Comparison of the Influence of the Solvent on the Extraction of the Bioactive Compounds from Marrubium lutescens Using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS)
    Sarikurkcu, C; Ozer, MS; Tlili, N
    To our knowledge, this is the first study on the influence of water, ethyl acetate and methanol as the extraction solvent upon the bioactive compound composition, antioxidant capacities and enzyme inhibitory activities of Marrubium lutescens isolates. The contents of the total phenolic compounds varied between 9.94 and 54.80 mg gallic acid equivalent/g extract and the level of total flavonoids were between 4.45 and 27.20 mg quercetin equivalent/g extract. Twenty-four compounds were identified using liquid chromatography - electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Rosmarinic acid, luteolin 7-glucoside, chlorogenic acid, caffeic acid and hyperoside were the primary compounds present for all of the used solvents. The most compounds (23) were detected in the methanol extract. Six antioxidant assays revealed that M. lutescens extracts showed interesting antioxidant activities with a significant variation between the extracts. The results revealed that the highest tyrosinase and alpha-amylase inhibitory activities was detected in the ethyl acetate extract. This work confirmed that the nature of solvent influenced the levels and the types of the extracted biomolecules. Moreover, the obtained results suggest that M. lutescens may be a source of bioantioxidants for industrial additives.
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    Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS) Identification of Phytochemicals and the Effects of Solvents on Phenolic Constituents, Antioxidant Capacity, Skin-Whitening and anti-Diabetic Activity of Onosma mitis
    Tlili, N; Sarikurkcu, RT; Ozer, MS; Sarikurkcu, C
    To the best of our knowledge, this is the first study on the effect of solvent (water, methanol, and ethyl acetate) upon phenolics extracted from Onosma mitis. The antioxidant capacities together with the enzyme inhibitory properties toward tyrosinase and alpha-amylase were also assessed. The yields of the extract were 2.62, 10.93, and 21.68% for ethyl acetate, methanol, and water, respectively. The concentrations of phenolic compounds were from 11.89 (ethyl acetate) to 2.66 mg gallic acid equivalent/g extract (methanol). Twenty-five molecules were identified and quantified by liquid chromatography - electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Significant differences were present between the extracts; methanol presented the highest number of compounds (25) while ethyl acetate provided the lowest (18). Chlorogenic acid, luteolin 7-glucoside, rosmarinic acid, and apigenin 7-glucoside were the major compounds regardless of solvent. Antioxidant and inhibitory enzyme assays showed that O. mitis exhibited interesting biological properties. The results illustrate the use of O. mitis as a promising source of biomolecules for industrial applications.
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    LC-ESI-MS/MS characterization of phytochemical and enzyme inhibitory effects of different solvent extract of Symphytum anatolicum
    Sarikurkcu, C; Ozer, MS; Tlili, N
    Symphytum anatolicum Boiss., is a species belonging to the Boraginaceae family and have been used for its curative effects. This work investigated, for the first time, the bioactive compounds, the antioxidant activities and the enzyme inhibitory effects of Symphytum anatolicwn roots extracts using three solvents (methanol, water and ethyl acetate). The LC-ESI-MS/MS was used to identify the individual bioactive compounds. Twenty-three biomolecules were identified in the extracts and rosmarinic acid, verbascoside, caffeic acid, chlorogenic acid and vanillin were the main identified with varied levels depending on the solvent used. Using six different assays, all extracts exhibited important antioxidant activities. Results showed also that the methanol extract exerted better tyrosinase inhibitory activities while ethyl acetate extract showed the best alpha-amylase inhibition. The obtained results highlighted the importance of S. anatolicum as a natural resource of bioactive compounds and encourage the use of this unexploited species for food additives, pharmaceutical industries, and skincare products.