Browsing by Author "Tuǧlu M.I."

Now showing 1 - 5 of 5
  • No Thumbnail Available
    Item
    In vitro effects of culture medium and serum on germ cells in testis and epididymis of male wistar rats; [Erkek wistar ratlarda kültür medyumunun ve serumun testis ve epididimis germinal hücreleri üzerine in-vitro etkileri]
    (2009) Cezayirli E.; Tuǧlu M.I.; Vural K.; Varol T.
    The present study was designed to examine the influence of culture media and serum on survival of in vitro rat sperms. The effects of different culture media with or without 10% fetal bovine serum (FBS) on spermatids and spermiums of Wistar rats were assessed in vitro between 2004 and 2005. Spermatozoa were cultured in Gamete-20, RPMI-1640, alphaMEM (AMEM), Dulbecco's modified Eagle's medium (DMEM) and Eagle's MEM (MEM). The number and morphology of cells was recorded at 4 and 24 h. Under all conditions the number and the viability of the cells decreased with time but parameters were positively affected by the presence of FBS. Viability rates of spermatids and spermatozoa reduced at 24 h of culturing. The survival and morphology were the best in RPMI with serum and worst in MEM without serum. These results indicate that different composition of culture media and FBS are important for maturation and survival of spermatozoa. Carefully selected culture media can play important roles in the generation of functional sperms as well as in the success of in-vitro fertilization (IVF) procedures.
  • No Thumbnail Available
    Item
    The contribution of differentiated bone marrow stromal stem cell-loaded biomaterial to treatment in critical size defect model in rats; [Biyomateryal üzerinde farklılaşmış kemik İliǧi stromal kök hücrelerinin kritik hacim defektli sıçan modelinde uygulanmasının tedavideki yeri]
    (Veteriner Fakultesi Dergisi, 2010) Tuǧlu M.I.; Özdal-Kurt F.; Koca H.; Sarac A.; Barut T.; Kazanç A.
    Mandibular fractures present a challenge in maxillo-facial surgery due to difficulties in healing and complications. In recent years, advances in bio-engineering as well as stem-cell studies suggest that it may be possible to treat these fractures by stem cell treatment with biomaterials. In the present study, we explored the efficacy of differentiated stem cells placed on biomaterials on fracture treatment and its relation with oxidative stress and apoptosis. A 4 mm circular defect was made on the mandibulae of 20 adults Wistar rats. Hydroxyapatite gel (control, n=5) and bone marrow stromal cells differentiated into osteoblast-seeded hydroxyapatite gel (n=5) were implanted within these defects. We were also used empty cavities (n=5) and cavities filled with only cells (n=5) for negative controls. Animals were sacrificed after a 6-week healing period and samples were examined blindly by histological, immunohistochemical, radiological and morphometric methods. Compared to the control cavities that underwent no procedure or filled with just cells, there were significant (P<0.001) healings in both groups. Hydroxyapatite gel with differentiated stem cells on, however, yielded significantly (P<0.05) better new bone formation and osteoid production decreased fibrous tissue and increased cellular activity. Differentiated stromal cells combined with biomaterial accelerated the treatment in defects of critical volume within a 6-week period of healing, activated and resulted in significant formation of bone of higher quality. Promotion of bone formation by the helps of bioengineering and stromal cells has gained importance in the treatment and reconstruction of fractures.
  • No Thumbnail Available
    Item
    The effects of fibroblast growth factor-2 blocking on development of chick cervical vertebra and relationship with oxidative stress and apoptosis; [Fibroblast growth faktör-2 bloklamasi{dotless}ni{dotless}n tavuk servikal omurlari{dotless} üzerindeki etkisi ve oksidatif stres ve apoptozis ile i̇lişkisi]
    (Ege University Press, 2014) Duransoy Y.K.; Şimşek T.; Öztürk F.; Mete M.; Tuǧlu M.I.; Selçuki M.
    Fibroblast growth factor (FGF) plays a role in the development of bone and cartilage. FGF-2 is a member of this family and blocking of FGF-2 affects the bone development. In this study, effects of FGF-2 blocking on the formation of vertebrae in chick embryos before ossifications and orientation of cervical vertebrae were investigated with histological examinations. In this study, anti-FGF-2 was performed to chick embryos at E4th and E10th days. Samples were taken in E15th days and changes in the cervical spine were evaluated as histochemically (with hematoxylin-eosin, Alizarin red, Masson's trichrome staining) and immunohistochemically (with iNOS, eNOS and TUNEL). Also changings were evaluated by morphometric analysis. In macroscopic examination of the cervical vertebrae significant difference was not detected. But in histochemical staining, defects were observed in cartilage and ossification process. Increasing in oxidative stress was demonstrated by iNOS and eNOS. Also apoptosis which was represented by tunnel was found to be increased. FGF-2 blocking affects the ossification process in the cervical spine by inducing oxidative stress and apoptosis which results with cell death.
  • No Thumbnail Available
    Item
    Effects of stem cells applications on oxidative stress and apoptosis during implantation
    (Wolters Kluwer Medknow Publications, 2018) Aydemir I.; Özkut M.M.; Firat F.; Gümürüdü A.; Sal D.H.; Erdoǧan K.; Göker A.; Tuǧlu M.I.
    Objective: To investigate the effects of bone marrow derived mesenchymal stem cell (BMSC) application into the rat endometrium on oxidative stress, cell proliferation and apoptosis. Methods: The female rats selected in estrous cycle were divided into three groups (saline, media and BMSC group). The intrauterine and intraperitoneal injections were performed using the saline (200 μL), culture media (200 μL) and 1×106 BMSCs/200 μL culture media, and then they were mated with male rats. On the 7th day of the pregnancy, uterine samples were harvested and dyed with heamatoxylin-eosin histochemically, anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase, and anti-proliferating cell nuclear antigen immunohistochemically, with terminal deoxynucleotidyl transferas dUTP nick end labeling for apoptosis. The stainings were evaluated by H-score and the results were analyzed using one-way ANOVA test statistically. Results: It was found that BMSCs increased the endometrial thickness, endometrial epithelium thickness and number of endometrial glands compared to control and sham groups. The intrauterine BMSC application decreased both anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase immunoreactivities and the number of apoptotic cells compared to the intraperitoneal applications whereas the immunoreactivity of proliferating cell nuclear antigen was increased. Conclusions: In current study, we define that stem cells do not cause any structural damages. Also they change the distribution of oxidative stress and cell proliferation marker. These findings support the reliability of stem cells in clinical use in the case of infertility. © 2018 by the Asian Pacific Journal of Reproduction. All rights reserved.
  • No Thumbnail Available
    Item
    Apoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells; [ORIGANUM MINUTIFLORUM'UN NB2A NÖROBLASTOMA HÜCRELERINE APOPTOTIK VE SITOTOKSIK ETKISI]
    (University of Ankara, 2020) Sari I.; Donmez Arat G.; Ozkan O.; Aydemir I.; Ozbey C.; Tuǧlu M.I.
    Objective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells. Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively. Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50doses were found to be10.75 μl/ml for oregano oil and 5μM for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels. © 2020 University of Ankara. All rights reserved.