Browsing by Author "Tugrul, B"
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Item Investigation of effect of vitamin D receptor, calcium-sensing receptor and β-catenin on cutaneous squamous cell carcinomaTugrul, B; Soylev, S; Temiz, P; Gençoglan, GBackground: Cutaneous squamous cell carcinoma (cSCC) is a malignant and invasive tumor which is originated from epidermis with a high incidence among non-melanoma skin cancers. The aim of this study was to determine whether vitamin D receptor (VDR), calcium-sensing receptor (CaSR) and beta catenin (beta-catenin) proteins have an effect on cSCC. Materials and methods: VDR, CaSR and beta-catenin proteins in tissue samples of cSCC and control group were analyzed by immunohistochemistry (IHC) and Western blotting (WB) method. IHC findings were statistically evaluated. Results: IHC staining density of VDR and beta-catenin were higher in cSCC tissue samples than control. The difference between IHC staining density of VDR and beta-catenin in the patient and the control groups were statistically significant (p = 0.021, p = 0.021, respectively), but not for CaSR (p = 0.237). While the VDR and beta-catenin staining rates obtained by the IHC method could be supported by WB results, the WB bands for CaSR could not be shown. Conclusion: The findings suggest that VDR and beta-catenin may have an effect on the disease. Further research is required to better understand the role of VDR and beta-catenin together on cSCC.Item Association Between Idiopathic Generalized Epilepsy and EFHC1 Gene Mutations of 662 G>A and 685 T>CBüyük, I; Tugrul, B; Yilmaz, H; Onur, E; Vatandas, G; Dogan Bozyigit, FObjective: Idiopathic generalized epilepsy (IGE) is an epilepsy form without an underlying brain lesion or neurological indication or symptom. Recent investigations on the genetic origins of IGE and its subtypes report that certain mutations of various ion and non-ion channel genes in the central nervous system may be associated with IGE. Among these mutations, the ones related to the non-ionic channel gene EFHC1 are controversial (545G>A, 685T>C, 628G>A 757G>T, 229C>A, 662G>A, 520A>G, 776G>A, 829C>T). In this study we investigated the relationship between IGE and 662G>A (R221H) and 685T>C (F229L) mutations in EFHC1 gene in a Turkish population. Material and Methods: The study enrolled 96 healthy volunteers (47 male, 49 female) and 96 IGE patients (41 male, 55 female). IGE diagnosis was confirmed in the neurology department. After venous blood sampling, DNA extractions were performed. The presence of 662G>A (R221H) and 685T>C (F229L) mutations in the exon 4 of EFHC1 gene were analyzed using Real-Time polymerase chain reaction (PCR) (Cobas, Roche Diagnostics, Germany). The results of the control and patient groups were compared statistically. Results: In the patient group there was one heterozygous male with 685T>C mutation. In the control group, there were two subjects with 685T>C mutation; one heterozygous male and one heterozygous female. The control and the patient groups did not have the 662G>A mutation. The difference between the patient and the control groups were not significant (p value for 685 T>C mutation=0.56062; p value for 662G>A mutation=1.00). Conclusion: We found no evidence that EFHC1 is a major genetic factor for the development of IGE in Turkish patients. Our results indicated that 685T>C and 662G>A mutations might not be associated with IJE.Item Prion protein-dependent regulation of p53-MDM2 crosstalk during endoplasmic reticulum stress and doxorubicin treatments might be essential for cell fate in human breast cancer cell line, MCF-7Tugrul, B; Balcan, E; Öztel, Z; Çöllü, F; Gürcü, BIn this study, we investigated the effect of doxorubicin and tunicamycin treatment alone or in combination on MDM-, Cul9-and prion protein (PrP)-mediated subcellular regulation of p53 in the context of apoptosis and autophagy. MTT analysis was performed to determine the cytotoxic effect of the agents. Apoptosis was monitorized by ELISA, flow cytometry and JC-1 assay. Monodansylcadaverine assay was performed for autophagy. Western blotting and immunofluorescence were performed to determine p53, MDM2, CUL9 and PrP levels. Doxorubicin increased p53, MDM2 and CUL9 levels in a dose-dependent manner. Expression of p53 and MDM2 was higher at the 0.25 & mu;M concentration of tunicamycin compared to the control, but it decreased at 0.5 & mu;M and 1 & mu;M concentrations. CUL9 expression was significantly decreased only after treatment of tunicamycin at 0.25 & mu;M. According to its glycosylation status, the upper band of PrP increased only in combination treatment. In combination treatment, p53 expression was higher than control, whereas MDM2 and CUL9 expressions were decreased. Combination treatments may make MCF-7 cells more susceptible to apoptosis rather than autophagy. In conclusion, PrP may be important in determining the fate of cell death through crosstalk between proteins such as p53 and MDM2 under endoplasmic reticulum (ER) stress conditions. Further studies are needed to obtain in-depth information on these potential molecular networks.Item Is Genetic Screening Necessary for Determining the Possibility of Venous Thromboembolism in Cancer Patients?Onur, E; Kurdal, AT; Tugrul, B; Iskesen, I; Dundar, P; Taneli, F; Ulman, C; Var, AObjective: To determine the risk of an association with some genetic polymorphisms involved in venous thromboembolism (VTE) gene variations (FVL, FV H1299R, FII G20210A, MTHFR C677T, MTHFR A1298C, PAI-1 4G/5G, beta-fibrinogen -455 G -> A, FXIII Val34Leu and GpIIIa HPA-1a) in cancer patients. Subjects and Methods: Among 78 cancer patients, 28 who had proven first episode of VTE were selected as the patient group, with 50 control samples selected from age-, sex-and body mass index-matched healthy volunteers (healthy group). The differences in frequency of genetic polymorphisms were found to be statistically insignificant between these two groups. Results: Logistic regression analysis after adjustment for age, sex, smoking and hypertension showed no difference. The screened mutations of these genes were not significantly associated with VTE risk. Conclusion: There is no possible benefit from genetic screening tests regarding VTE in cancer patients. Copyright (C) 2011 S. Karger AG, BaselItem The role of hydroxytyrosol in health and diseaseTugrul, B; Oktay, LMHydroxytyrosol is a phenolic compound released from the hydrolysis of oleuropein and is present in olive oil, leaf and fruit. Recently, studies on hydroxytyrosol have revealed its strong antioxidant and antiinflamatory effects. In vitro studies conducted in various cancer cell lines and in vivo assays carried out on animals reported strong evidences for its antiinflamatory, antiproliferative and proapoptotic influences. In addition, in cancer it is believed to have inhibitory effects during angiogenesis. Its modulatory role on bone formation and prevention of bone loss has been the attraction of some studies. If the molecular mechanisms and cellular pathways of hydroxytyrosol are elucidated, it will be possible to plan new strategies targeting various disease involved with cancer, inflammatory and cardio-vascular pathologies. In this review, various in vitro and in vivo studies investigating the antioxidant, anticancer, antiinflamatory role of hydroxytyrosol and its inhibitory effects in osteoporosis are discussed.