Browsing by Author "Turkoz, E"

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    Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma
    Sanci, M; Dikis, C; Inan, S; Turkoz, E; Dicle, N; Ispahi, C
    Angiogenic factors, such as vascular endothelial growth factor (VEGF), its receptors and epidermal growth factor receptor (EGF-R), are involved in increased progression in many carcinomas. The aim of this study was to investigate the role of angiogenesis and immunolocalization of VEGF, its receptors, EGF-R and Ki 67 in leiomyomas and leiomyosarcomas using an indirect immunohistochemical method. Samples from patients with leiomyoma, cellular leiomyoma and cellular leiomyosarcoma (n=20 per group) were fixed in 10% formalin and processed using routine paraffin protocols. Following initial histological analysis, samples were immunostained with primary antibodies for VEGF, VEGFR-1, VEGFR-2, EGF-R and Ki-67 using an indirect avidin-biotin peroxidase method. Immunostaining intensities were evaluated as mild, moderate or strong and a semi-quantitative method (H-Score) was used to compare the samples. While mild/moderate EGF-R immunostaining and moderate immunostaining for VEGF and its receptors were observed in samples of leiomyomas, much less immunoreactivity was observed in cellular leiomyomas. All immunoreactivities and immune-stained cells increased in leiomyosarcomas. When scores of intensity and percentage of positive staining cells were compared, all immunoreactivities were shown to be significantly increased in leiomyosarcomas compared to leiomyomas. These results suggest that in leiomyosarcoma, angiogenic factors, such as VEGF, its receptors and EGF-R, may be involved in tumor angiogenesis. Active tumor cells can trigger angiogenesis, interaction with surrounding tissue and in the tissue itself initiating angiogenic activity. Angiogenic growth factors play an important role and induce malignant transformation through both autocrine and paracrine mechanisms. Anti-angiogenic agents may provide a novel therapeutic approach for the treatment of leiomyosarcoma. (C) 2010 Elsevier GmbH. All rights reserved.
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    Effect of atorvastatin and lisinopril or their combination on renal tissue damage in unilateral ureteral obstruction in rats
    Karabuga, L; Tuma, B; Vatansever, S; Altay, B; Ekren, F; Turkoz, E; Ustun, G; Nazli, O; Apaydin, E
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    Defective epithelial barrier function related to zonula occludens proteins of nasal mucosa in subjects with atopy
    Yilmaz, O; Inan, S; Pinar, E; Turkeli, A; Turkoz, E; Kanik, ET; Yuksel, H
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    Inhibitory Effects of Propolis on Human Osteogenic Sarcoma Cell Proliferation Mediated by Caspase Patway
    Kurt, FO; Vatansever, HS; Sorkun, K; Gurhan, SID; Turkoz, E; Gencay, O; Salih, B
    A natural product Propolis, is a resinous material gathered by honeybees from the buds and bark of certain trees and plants. Propolis contains various chemical components of biological activities, including antimutagenic, antioxidant, antibacterial, antiviral and anticarsinogenic. Therefore, the aim of this study is to investigate the antiapoptotic effect of propolis extracts (PE) using caspase pathway in the human osteogenic sarcoma cell line SAOS-2 in culture. The extracts which produced in ecologic environment were taken from the Hacettepe University, Beytepe Campus area-Ankara were used. Seven different PE at 0.5, 0.25, 0.125 and 0.063 mg/ml were added to SAOS-2 cell line for two days incubation. For cell proliferation and cytotoxicty analyses MTT, for apoptotic cell death determination TUNEL method, for distribution of caspase 6, caspase 8 and caspase 9 indirect immunocytochemistry analyses were used. After MTT analyses, the most effect was observed PE 7 at the 0.125 mg/ml dilution. The number of TUNEL positive cells was more detectable at PE 4 and 5 at the 0.063 mg/ml, and PE 7 at the 0.125 mg/ml dilutions. The immunoreactivity of caspase 6 was stronger than caspase 8 and 9. Moreover, density of caspase 6 staining was much better especially in PE 7 at the 0.125 mg/ml dilution. In conclusion, the mechanisms of apoptosis induction by PE may appear via caspase pathway because of its anticanserogenic effect. PE may be usefull in the cancer treatment protocol.
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    Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines
    Vatansever, HS; Sorkun, K; Gurhan, SID; Ozdal-Kurt, F; Turkoz, E; Gencay, O; Salih, B
    Propolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects The target of this study was to investigate the anti apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF 7 in culture Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey Individual extracts at 0 5, 0 25, 0 125 and 0 063 mg/ml were incubated with MCF 7 cells during 2 days culture Cell growth and cytotoxicity were measured colorimetrically by MTT assay Apoptotic cell death was determined by the TUNEL method (terminal deoxynucleotidyltransferase-biotin nick end labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9 The results showed that the PE 5 and 6 extracts at 0 125 mg/ml dilution induced apoptosis in association with increased number of TUNEL positive cells MTT results showed that cultures exposed to the same extracts and at the same dilution experienced better cell growth compared to those cultures exposed to the other extracts Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9 Caspase 6 labelling was especially strong in PE 5 and PE 6 We conclude that propolis may have anti-tumour effects by increasing apoptosis through the caspase pathway Such propolis extracts may be important economically and allow development of a relatively inexpensive cancer treatment (C) 2009 Elsevier GmbH All rights reserved