Browsing by Author "Yücecan S."

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    Quercetin-mediated apoptosis and cellular senescence in human colon cancer
    (Bentham Science Publishers, 2020) Özsoy S.; Becer E.; Kabadayı H.; Vatansever H.S.; Yücecan S.
    Background: Quercetin is a flavonol from the flavonoid group of polyphenols, which positively affects human health due to its anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects of phenolic compounds, including quercetin, on programmed cell death and cellular senescence, have been the subject of research in recent years. Objective: In this study, we aimed to investigate the effects of quercetin on cell viability, apoptosis and cellular senescence in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines. Methods: Cytotoxicity was analyzed via MTT assay in Colo-320 and Colo-741 cell lines. After quercetin treatment, cell ularsenescence and apoptosis were evaluated by TUNEL staining, X-Gal staining and indirect peroxidase tech-nique for immunocytochemical analysis of related proteins such as Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: The effective dose for inhibition of cell growth in both cell lines was determined to be 25µg/ml quercetin for 48 hours. Increased Baximmunoreactivityfollowingquercetin treatment was significant in both Colo-320 and Colo-741 cell lines, but decreased Bcl-2 immunoreactivitywas significant only in theColo-320 primary cell line. In addition, after quercetin administration, the number of TUNEL positive cells and, immunoreactivities for p16, Lamin B1 and cyclin B1 in both Colo-320 and Colo-741 cells increased. Conclusion: Our results suggest that quercetin may only induce apoptosis in primary colon cancer cells. Further-more, quercetin also triggered senescence in colon cancer cells, but some cells remained alive, suggesting that colon cancer cells might have escaped from senescence. © 2020 Bentham Science Publishers.
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    The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell
    (Routledge, 2020) Hoca M.; Becer E.; Kabadayı H.; Yücecan S.; Vatansever H.S.
    Resveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol. © 2020, © 2019 Taylor & Francis Group, LLC.
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    Resveratrol triggers Apoptosis in colon cancer cells rather than senescence
    (Mattioli 1885, 2021) Madencioğlu S.; Becer E.; Kabadayı H.; Vatansever H.S.; Yücecan S.
    Objective: Resveratrol is a phenolic compound that classified in stilbenoid and used as anticancer agent in many cancer types. The purpose of the study is to determine apoptotic and senescence inducing effects in primary (Colo-320) and metastatic (Colo-741) colon cancer cells. Methods: Cell growth and cytotoxicity were detected by MTT method in both Colo-320 and Colo-741 cell lines. Apoptotic and senescence inducing activities were tested with TUNEL staining, X-gal staining and immunocytochemistry using antibodies directed against to Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: According to MTT results, 25 μg/mL and 10 μg/mL concentrations of resveratrol were found more effective for Colo-320 and Colo-741 cell lines, respectively. As a result of immunocytochemical staining, Bax immunoreactivity was significantly increased while Bcl-2 immunoreactivity significantly decreased in Colo-320 cell line. Increased Hsp27, lamin B1 and p16 immunoreactivities on Colo-320 cells were not significant. In Colo-741 cells, Bcl-2 immunoreactivity was significantly increased. Hsp27 immunoreactivity in Colo-320 cell line was significantly higher than Colo-741 cell line. In addition, after resveratrol administration, while the TUNEL positive cells significantly increased in Colo-320 cells, X-gal positive cells was detected in Colo-741 than Colo-320 cells. Conclusion: Resveratrol can inhibit cell viability both in primer and metastatic colon cancer cells. However, resveratrol might be more effective triggering mitochondrial-mediated apoptosis in primary colon cancer cells. Apoptotic and cell cycle inhibiting effects of resveratrol may differ by cell type. Therefore, resveratrol may be a potential phytotherapeutic agent for colon cancer according to the cell origin. © Mattioli 1885.
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    Neuroprotective effects of catechins in an experimental Parkinson’s disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects
    (Taylor and Francis Ltd., 2022) Özduran G.; Becer E.; Vatansever H.S.; Yücecan S.
    Objectives: The aim of the study was to establish an in vitro Parkinson’s disease (PD) model and to investigate the cell viability, anti-inflammatory, anti-apoptotic and neuroprotective effects of catechin and EGCG in SK-N-AS and in vitro PD model cells. Method: SK-N-AS human neuroblastoma cells were used. To develop an in vitro PD model, SK-N-AS cells were exposed to 6-hydroxydopamine. Model control was performed after ELISA analysis of dopamine and α-synuclein levels in the culture medium. Catechin and EGCG were administered to SK-N-AS and in vitro PD model cells. Cell viability was measured using MTT assay and trypan blue staining. Anti-inflammatory and anti-apoptotic activities of catechin and EGCG were investigated by indirect immunocytochemistry using anti-TNF-α, anti-IL-1β and anti-caspase-3. Results: After 24 hours of 6-hydroxydopamine administration at 50 μM, higher αlfa-synuclein and lower dopamine levels were found in PD model than SK-N-AS cells. Cell viability was similar between SK-N-AS and in vitro PD model cells. Treatment with both bioactive components increased cell viability of in vitro PD model cells. Caspase-3 immunoreactivity was significantly reduced in SK-N-AS and PD model cells after EGCG administration, while it was decreased only in PD model cells after catechin administration. IL-1β staining intensity weakened after catechin administration in PD model cells, after EGCG administration in SK-N-AS cells. TNF-α staining intensity was similar in both cells. Conclusion: Catechin and EGCG increased cell viability in PD model neuron cells. Both components showed anti-apoptotic and anti-inflammatory effects. Catechin may be more effective in preventing damage to neurons PD. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
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    Neuroprotective Effects of Hesperidin and Naringin in SK-N-AS Cell as an In Vitro Model for Alzheimer’s Disease
    (Routledge, 2023) Kuşi M.; Becer E.; Vatansever H.S.; Yücecan S.
    Objective: Hesperidin and naringin are flavonoids that are found in citrus fruits. Our aim was to create an in vitro model of Alzheimer’s disease (AD) and to evaluate the neuroprotective effects of hesperidin and naringin in SK-N-AS and AD model cells. Methods: Aβ25-35 was used to create an AD model in SK-N-AS cells. The cytotoxicity of hesperidin and naringin was evaluated using MTT. β-amyloid, tau and α-synuclein distributions were analyzed using indirect immunoperoxidase staining to investigate the neuroprotective effects of hesperidin and naringin. Results: The AD model was created by 1 µM of Aβ25-35 for 48 hours after ThT staining. The intensity of β-amyloid was reduced through both hesperidin and naringin treatment in AD model cells. Both flavonoids significantly decreased the intensity of α-synuclein in SK-N-AS and AD model cells. Conclusions: Hesperidin and naringin can be potentially used as neuroprotective agents. Naringin may be more effective than hesperidin in the accumulation of β-amyloid and tau proteins. © 2022 American College of Nutrition.