Browsing by Author "Yasa, I"

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    Production of laccase from Trametes trogii TEM H2: a newly isolated white-rot fungus by air sampling
    Kocyigit, A; Pazarbasi, MB; Yasa, I; Ozdemir, G; Karaboz, I
    This work represents the first report of isolation of potential laccase producers by air sampling using media supplemented with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol for laccase production and secretion indicators. Nine fungal isolates showed positive reactions with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol. The isolate named TEM H2 exhibited the largest and intensive oxidation zones with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (85 mm) and guaiacol (66 mm) and therefore it was selected for detailed investigations. The strain was identified as Trametes trogii TEM H2 due to the morphological characteristics and the comparison of internal transcribed spacer ribosomal DNA gene sequences. The laccase production was screened in different liquid cultures. The best laccase production medium was determined as soluble starch yeast extract medium in which laccase production was reached to a maximum level (989.6 U l1) on the 8th day of cultivation. Effects of different initial pH values on laccase production were tested. Optimum pH value for laccase production in soluble starch yeast extract medium was determined as pH 3.0 with 15425.0 U l1laccase production at 12th day of cultivation. In addition, effects of eight inducers (veratryl alcohol, ferulic acid, 1-Hydroxybenzotriazole, syringic acid, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate), 1 mmol l1 CuSO4, 3% ethanol, guaiacol) were examined. Only cultures with 2,5-xylidine exhibited 1.9 fold increase in laccase activity reaching to 28890.0 U l1. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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    DECOLORIZATION OF VARIOUS LEATHER DYES AND LEATHER INDUSTRY EFFLUENT BY Trametes trogii TEM H2
    Pazarbasi, MB; Kocyigit, A; Ozdemir, G; Yasa, I; Karaboz, I
    Decolorization of Acid Blue 7 which is used widely in leather industry was investigated as a model for a decolorization system using soluble starch yeast extract medium under agitated and static conditions with Trametes trogii TEM H2. The effects of different physico-chemical parameters were tested and optimal decolorization rates occurred at pH 5.0 and at 27 degrees C. Decolorization of Acid Blue 7 under agitated and static conditions was determined to be 99.9% and 63.5%, respectively. Decolorization was associated with laccase activity which reached 1110.3 U/L in agitated cultures in the presence of Acid Blue 7 on the 6th day of cultivation. T. trogii TEM H2 was further evaluated for the decolorization of 8 other leather dyes, such as Acid Black 210, Acid Green 20, Acid Yellow 36, Acid Black 24, Acid Black 234, Acid Violet 17, Acid Blue 134, Acid Brown 349, and a mixture of Acid Blue 7 with these 8 leather dyes and leather industry effluents. The decolorization rates after 24 h for the dye mixture and the effluent (10%) were 88% and 48%, respectively. The strain was considered as a good candidate for biodegradation and bioremediation of leather dye-polluted effluents due to its laccase production and decolorizing ability.
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    APOLAR CONSTITUENTS OF SOME BIOLOGICALLY ACTIVE Dianthus SPECIES FROM WESTERN ANATOLIA
    Durucasu, I; Mutlu, K; Sik, L; Yasa, I; Arda, N; Kirmizigul, S
    The apolar constituents of four Dianthus (Caryophyllaceae) species were determined by GC-MS. Palmitic, linoleic, and oleic acids were detected as dominant components in all species. D. elegans d'Urv. var. elegans had the highest antioxidant activity. All four species also showed considerable antimicrobial activity against S. epidermidis and C. albicans.
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    Synthesis and antimicrobial evaluation of sulfanilamide- and carbohydrate-derived 1,4-disubstitued-1,2,3-triazoles via click chemistry
    Ay, K; Ispartaloglu, B; Halay, E; Ay, E; Yasa, I; Karayildirim, T
    4-Sulfanilamido substitued-1,2,3-triazoles conjugated with monosaccharides (8-17) including d-glucose, d-galactose, d-mannose, and d-fructose were synthesized in good yields from azidosugars with propargyl sulfanilamides using copper catalyst 1,3-dipolar cycloaddition reaction (CuAAC). The structures of new compounds were elucidated by liquid chromatography-mass spectrometry, infrared, one-dimensional- and two-dimensional-nuclear magnetic resonance techniques. All of the new compounds were tested in vitro against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans for their antibacterial and antifungal activities. Experimental results showed antimicrobial activity with minimum inhibitory concentrations values a ranging from 0.078 to 5.0 mg/mL against test microorganisms.
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    Ligninolytic enzyme activities in mycelium of some wild and commercial mushrooms
    Kalmis, E; Yasa, I; Kalyoncu, F; Pazarbasi, B; Kocyigit, A
    Lignin is probably one of the most recalcitrant compounds synthesized by plants. This compound is degraded by few microorganisms. White-rot fungi have been extensively studied due to its powerful ligninolytic enzymes. In this study, ligninolytic enzyme activities of different fungal species (six commercial and 13 wild) were investigated in solid and liquid culture media. It was postulated that, among the wild strains, only Pleurotus ostreatus-1 (MCC45), P. ostreatus-2 (MCC40), Pleurotus eryngii-1 (MCC25) and P. eryngii-2 (MCC26), and commercial strains P. ostreatus, P. sajor-caju, P. eryngii presented lignin peroxidase (LiP) activity. All enzymes tested in this study were not determined in Rigidoporus ulmarius, Gloeophyllum trabeum and Tricholoma caligatum as well as commercial strain Pleurotus citrinopileatus. Therefore, the results of the present study allow us to conclude that wild P. ostreatus and P. eryngii are good candidates for scale-up ligninolytic enzyme production.