Browsing by Author "Yavuz, DÖ"

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    Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells
    (GALENOS PUBL HOUSE) Yavuz, DÖ; Becer, E; Kendirci, R; Kurt, FÖ; Vatansever, HS
    BACKGROUND/AIMS The aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CDI33+ Colo-320) or nonstem (CDI33- Colo-320) cells. MATERIAL and METHODS T. capitatus essential oil was obtained by water distillation and analyzed by GC-MS. Cancer stem cells (CDI33+ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system. Proapoptotic activity of T capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTS Caspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CDI33+ and CDI33- Colo320 cells. CONCLUSION We conclude that T capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis. Interestingly, T capita- tus essential oil is found to be more effective in Colo-320 cells than CDI33+ Colo-320 and CDI33- Colo-320 cells in terms of apoptosis.
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    ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL OF THYMUS CAPITATUS AGAINST HELICOBACTER PYLORI
    (POLSKIE TOWARZYSTWO FARMACEUTYCZNE) Güvenir, M; Hanoglu, D; Hanodlu, A; Süer, K; Baser, HC; Yavuz, DÖ; Salidag, T
    Helicobacter pylori (H.pylori) is an infective agent and the main resourceful factor of chronic, active type B gastritis, peptic and duodenal ulcer, gastric carcinoma and mucosa-associated lymphoid tumors. Nowadays, pharmcological treatment of H. pylori infections attracts attention besides antibiotic treatment. The aim of this study is to analyse the chemical composition of the essential oils of Thymus capitatus and the antimicrobial activity against H. pylori. Aerial parts of wild-growing plant materials were collected during the flowering stage from three different locations. Essential oil composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the essential oil was tested by broth dilution method. The thymol percentage was determined as more than half of the essential oil composition of Thymus capitatus-Yedidalga (51.9%) and Thymus capitatus-Yildirim (57.1%); however, the composition of thymol was 47.2% followed by 15.1% (p-cymene), 10.0% (gamma-terpinene) and 5.7% (carvacrol) for Thymus capitatus-Bogaz/Girne. The essential oil from Thymus capitatus-Bogaz/Girne showed the lowest MIC and MBC values and the strongest bactericidal activity against H. pylori compared to the other tested essential oils. Bactericidal efficacy of essential oil of Thymus capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus essential oil could be an alternative way for the treatment of H. pylori infections.
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    English
    (0378-1119) Becer, E; Hanoglu, DY; Kabadayi, H; Hanoglu, A; Vatansever, S; Yavuz, DÖ; Meriçli, F; Meriçli, AH
    ELSEVIER SCIENCE BV
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    English
    (1129-8723) Yavuz, DÖ; Becer, E; Vatansever, HS; Kabadayi, H; Hanoglu, A; Meriçli, F; Meriçli, AH
    MATTIOLI 1885