Browsing by Author "Yavuz, DÖ"

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    Cellular Behavior of Colchicum troodi Treated Primary and Metastatic Colon Adenocarcinoma Cell Lines
    Yavuz, DÖ; Becer, E; Vatansever, HS; Kabadayi, H; Hanoglu, A; Meriçli, F; Meriçli, AH
    Colchicum troodi belongs to Colchicaceae family that particularly rich in flavonoids, phenolic acids, tannin, fatty acids and alkaloids such as colchicine. The aim of this study was to investigate the anti-proliferative and protective effects of Colchicum troodi ethanolic extract toward relevant molecular signalling pathways on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Colchicum troodi was collected and extracted. Different concentrations of Colchicum troodi extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of Colchicum troodi were investigated by immunocytochemistry using antibodies directed against to beta-catenin, LGRS, jagged 1, IHH, CD133 and Ki-67 in Colo-320 and Colo-741 cells. In the MTT assay, 10 mu g/ml and 5 mu g/ml Colchicum troodi extract were found to be active against Colo-741 and Colo-320 cells, respectively. Colchicum troodi extract significantly increased caspase beta-catenin immunoreactivities while IHH immunostaining intensity was decreased in Colo-741 cells. We conclude that Colchicum troodi extract increased beta-catenin via Wnt/beta-catenin pathway in Colo-741 cells. Interestingly, it decreased IHH immunoreactivities which is an antagonist of constitutive activity of Wnt/ beta-catenin signaling and is assumed to play protective act during carcinogenesis.
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    The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway
    Becer, E; Hanoglu, DY; Kabadayi, H; Hanoglu, A; Vatansever, S; Yavuz, DÖ; Meriçli, F; Meriçli, AH
    Objective: Colchicum pusillum belongs to the family Colchicaceae that particularly rich in tropolonic alkaloids. The aim of this study was to investigate the cytotoxicity and in vitro anticancer activity of Colchicum pusillum ethanolic extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Materials and methods: Colchicum pusillum was collected and extracted with ethanol. Different concentrations of Colchicum pusillum extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Anticancer and antiproliferative activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to beta-catenin, Ki-67, LGR-5 Ki-67, DKK1, Frizzled-4, Wnt4, Wnt7a and caspase3 in Colo-741 cells. Results: All concentrations of Colchicum pusillum extract had toxic effect in Colo-320 cells. Because of this, we used Colchicum pusillum extract at 20 mu g/ml for evaluate anticancer activities only in Colo-741 cells. As a result of immunohistochemical staining, beta-catenin, LGR-5 and caspase-3 immunoreactivities were significantly increased while Wnt7a immunostaining intensity was decreased in Colo-741 cells. Conclusion We conclude that Colchicum pusillum extract increased beta-catenin and LGR-5 via Wnt/beta-catenin pathway in colon cancer cells. Interestingly, it decreased other signaling molecule, Wnt7a which is assumed to play protective role during carcinogenesis. Also, it increased significantly caspase-3 immunoreactivity showing that apoptotic pathways were triggered.
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    ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL OF THYMUS CAPITATUS AGAINST HELICOBACTER PYLORI
    Güvenir, M; Hanoglu, D; Hanodlu, A; Süer, K; Baser, HC; Yavuz, DÖ; Salidag, T
    Helicobacter pylori (H.pylori) is an infective agent and the main resourceful factor of chronic, active type B gastritis, peptic and duodenal ulcer, gastric carcinoma and mucosa-associated lymphoid tumors. Nowadays, pharmcological treatment of H. pylori infections attracts attention besides antibiotic treatment. The aim of this study is to analyse the chemical composition of the essential oils of Thymus capitatus and the antimicrobial activity against H. pylori. Aerial parts of wild-growing plant materials were collected during the flowering stage from three different locations. Essential oil composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the essential oil was tested by broth dilution method. The thymol percentage was determined as more than half of the essential oil composition of Thymus capitatus-Yedidalga (51.9%) and Thymus capitatus-Yildirim (57.1%); however, the composition of thymol was 47.2% followed by 15.1% (p-cymene), 10.0% (gamma-terpinene) and 5.7% (carvacrol) for Thymus capitatus-Bogaz/Girne. The essential oil from Thymus capitatus-Bogaz/Girne showed the lowest MIC and MBC values and the strongest bactericidal activity against H. pylori compared to the other tested essential oils. Bactericidal efficacy of essential oil of Thymus capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus essential oil could be an alternative way for the treatment of H. pylori infections.
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    Proapoptotic and Anticancer Potentials of Thymus capitatus Essential Oil on Colon Cancer Stem Cells
    Yavuz, DÖ; Becer, E; Kendirci, R; Kurt, FÖ; Vatansever, HS
    BACKGROUND/AIMS The aim of this study was to investigate the proapoptotic activity of Thymus capitatus essential oil in either colon cancer stem (CDI33+ Colo-320) or nonstem (CDI33- Colo-320) cells. MATERIAL and METHODS T. capitatus essential oil was obtained by water distillation and analyzed by GC-MS. Cancer stem cells (CDI33+ Colo-320) were obtained from the Colo-320 cells by the MiniMACS system. Proapoptotic activity of T capitatus essential oil was investigated by immunocytochemistry using antibodies directed against caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. RESULTS Caspase-3 immunoreactivity was significantly increased in 0.5% dilution T. capitatus essential oil-treated Colo-320 cells for 48 hours Moreover, the number of TUNEL positive cells was significantly higher in Colo-320 cells when compared with CDI33+ and CDI33- Colo320 cells. CONCLUSION We conclude that T capitatus essential oil increases caspase-3 molecules, which play a crucial role in apoptosis. Interestingly, T capita- tus essential oil is found to be more effective in Colo-320 cells than CDI33+ Colo-320 and CDI33- Colo-320 cells in terms of apoptosis.