Browsing by Author "Yavuz D.Ö."

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    Antimicrobial activity of the essential oil of Thymus capitatus against Helicobacter pylori
    (Polish Pharmaceutical Society, 2020) Güvenir M.; Hanoğlu D.; Hano-Lu A.; Süer K.; Başer H.C.; Yavuz D.Ö.; Şalidağ T.
    Helicobacter pylori (H.pylori) is an infective agent and the main resourceful factor of chronic, active type B gastritis, peptic and duodenal ulcer, gastric carcinoma and mucosa-associated lymphoid tumors. Nowadays, pharmcological treatment of H. pylori infections attracts attention besides antibiotic treatment. The aim of this study is to analyse the chemical composition of the essential oils of Thymus capitatus and the antimicrobial activity against H. pylori. Aerial parts of wild-growing plant materials were collected during the flowering stage from three different locations. Essential oil composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the essential oil was tested by broth dilution method. The thymol percentage was determined as more than half of the essential oil composition of Thymus capitatus-Yedidalga (51.9%) and Thymus capitatus ñYıldırım (57.1%); however, the composition of thymol was 47.2% followed by 15.1% (p-cymene), 10.0% (γ-terpinene) and 5.7% (carvacrol) for Thymus capitatus-Boğaz/Girne. The essential oil from Thymus capitatus- Boğaz/Girne showed the lowest MIC and MBC values and the strongest bactericidal activity against H. pylori compared to the other tested essential oils. Bactericidal efficacy of essential oil of Thymus capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus essential oil could be an alternative way for the treatment of H. pylori infections. © 2020 by Polish Pharmaceutical Society.
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    Cellular behavior of Colchicum troodi treated primary and metastatic colon adenocarcinoma cell lines
    (Mattioli 1885, 2021) Becer E.; Yavuz D.Ö.; Vatansever H.S.; Kabadayı H.; Hanoğlu A.; Meriçli F.; Meriçli A.H.
    Colchicum troodi belongs to Colchicaceae family that particularly rich in flavonoids, phenolic acids, tannin, fatty acids and alkaloids such as colchicine. The aim of this study was to investigate the anti-proliferative and protective effects of Colchicum troodi ethanolic extract toward relevant molecular signalling pathways on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Colchicum troodi was collected and extracted. Different concentrations of Colchicum troodi extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of Colchicum troodi were investigated by immunocytochemistry using antibodies directed against to β-catenin, LGR5, jagged 1, IHH, CD133 and Ki-67 in Colo-320 and Colo-741 cells. In the MTT assay, 10 µg/ml and 5 µg/ml Colchicum troodi extract were found to be active against Colo-741 and Colo-320 cells, respectively. Colchicum troodi extract significantly increased caspase β-catenin immunoreactivities while IHH immunostaining intensity was decreased in Colo-741 cells. We conclude that Colchicum troodi extract increased β-catenin via Wnt/β-catenin pathway in Colo-741 cells. Interestingly, it decreased IHH immunoreactivities which is an antagonist of constitutive activity of Wnt/ β-catenin signaling and is assumed to play protective act during carcinogenesis. © Mattioli 1885