Browsing by Author "Yeǧenoǧlu E.D."
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Item RAPD-PCR analysis of cultured type olives in Turkey(2009) Sesli M.; Yeǧenoǧlu E.D.The aim of this study was to detect genetic similarities and distances among cultured type olive trees by RAPD-PCR technique. Olives are raised in a high range from the Aegean, Mediterranean, Marmara and Black Sea to Southeast Anatolia regions of Turkey. Olive breeding had a rapid increase in Turkey during recent years, among the agricultural products. Finding the genetic relationships between cultured type olives may help to improve genetic resources and our knowledge of their evolutionary background and to determine genetic relationships with wild type relatives. In this study, samples were obtained from the Olive Production Research Institute (Manzanilla, Domat, Gemlik and Memecik) and sapling producers in Manisa, Akhisar (Uslu, Edremit). Genomic DNA's were extracted from young leaves and PCR was used generate RAPD bands. Sixty random primers obtained from Operon Tech. were tested by RAPD-PCR (OP-A, OP-I, OP-Q). A total of 36 primers among 60 Operon random primers (Kit OP-A, OP-I, OP-Q) yielded clear and firm bands. The electrophoretic patterns of olive samples showed that 291 highly polymorphic loci. Averages of 8.08 scorable bands per primer were determined from RAPD-PCR analysis. © 2009 Academic Journals.Item Genetic analysis on wild olives by using RAPD markers(2009) Sesli M.; Yeǧenoǧlu E.D.The aim of this study was to detect genetic similarities and distances among wild olive trees by RAPDPCR technique. Olives are raised at a high range from Artvin on the north, up to Hatay on the south and up to Mardin on the Southeast part of Turkey. The Aegean, Mediterranean, Marmara and Black Sea regions of Turkey have suitable climatic conditions for olive breeding. Olive breeding had a rapid increase in the Aegean Region during recent years, among the agricultural products. Therefore, it is required to know the genetic relationships between wild olives and their cultural relatives in order to improve genetic resources and our knowledge of their evolutionary background. In this study, samples were mainly collected from Manisa province and additionally, samples were taken from Izmir and Mugla provinces for comparison. Genomic DNA's were extracted from young leaves and PCR was used to generate RAPD bands. 60 random primers obtained from Operon Tech. were tested by RAPD-PCR. Total of 38 primers were given highly polymorphic and continuous scorable bands. 167 bands were found and each primer was screened at an average of 4.4 polymorphic bands. © 2009 Academic Journals.Item Standardization of RAPD assay for genetic analysis of olive(2009) Sesli M.; Yeǧenoǧlu E.D.This study aimed at the standardization of PCR conditions for the purpose of determining the genetic similarities and distances in wild and cultivated olives collected from Izmir and Manisa provinces in Aegean Region. Three different PCR mix and three different PCR cycle as well as 20 primers from OP-I series were tried in 13 wild and 12 cultivated samples. A suitable PCR protocol was developed and evaluable bands were derived for further studies. Required result could not be obtained from PCR Mixes I and II and also PCR cycles I and II; however, favorable results were attained by PCR Mix III and PCR cycle III. Evaluable bands were obtained for defining the olive samples by using primers from OP-I. Thus, it was concluded that RAPD profiles are effective in the study of genetic similarities and distances of wild and cultivated olives. © 2009 Academic Journals.Item RAPD assay of wild-type olives in Turkey(Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59. The genetic distance values ranged from 0.1498 to 0.6845, and genetic similarity values varied from 0.8609 to 0.5043. We found that the closest samples based on their genetic distance and similarity values were from Harlak and Sabancilar; the most distant samples were from Bornova and Bademli, © FUNPEC-RP.Item Determination of the genetic relationships between wild olive (Olea europaea oleaster) varieties grown in the aegean region(Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.The RAPD technique was used for determining genetic differences between 12 wild-olive varieties grown in the Aegean provinces of Izmir, Mugla, and Manisa in Turkey. Wild olives obtained from the same provinces were included in the same plot. Twenty of 25 operon primers (OP-I 4, OP-I 14, OP-I 15, OP-I 16, OP-I 17, OP-Q1, OP-Q2, OP-Q3, OP-Q4, OP-Q11, OP-Q12, OP-Q13, OP-Q14, OP-Q15, OP-Q16, OP-Q17, OP-Q18, OP-Q19, OP-Q20, OP-F1, OP-F2, OP-F3, OP-F6, OP-F7, OP-F8) yielded bands. The differences between the varieties were determined based on their genetic similarities, using principal coordinate analysis; genetic distances were determined using neighbor-joining analysis. The varieties wild 7 and wild 12 had the lowest genetic similarity (0.97, Jaccard similarity index); they also had the greatest genetic distance between them (0.3606, Nei's genetic distance). It was concluded that the RAPD technique is adequate for the evaluation of genetic relationships among wild olives. Principal coordinate analysis and neighbor-joining analysis gave results that support the Genetic relationships between wild olive varietie use of this type of analysis to help understand the genetic background of olives and for further genetic studies. ©FUNPEC-RP.Item Genetic relationships among and within wild and cultivated olives based on RAPDs.(2010) Sesli M.; Yeǧenoǧlu E.D.We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP-F 14-15-16-17, and OP-K 1-8) and 49 polymorphic bands were selected and used for analysis. The dendrogram based on unweighted pair-group cluster analysis using the Sorensen-Dice coefficient of similarity index indicated two major groups, dividing wild olives from cultivated olives. The patterns of genetic relationships among and within the different olives were analyzed by means of analysis of molecular variance. We found significant differences between wild and cultivated olives (Phi(st) = 0.1507; P < 0.001). In order to determine the genetic relationship among wild and cultivated olives, principal coordinate analysis was used to examine the variation among subspecies. The wild and cultivated olives formed two main groups, one on the right side and the other on the left side of the principal coordinates graph, respectively. This was compatible with the results we obtained from analysis of molecular variance.Item Comparison of Manzanilla and wild type olives by RAPD-PCR analysis(Academic Journals, 2010) Sesli M.; Yeǧenoǧlu E.D.The object of this study was to detect genetic similarities and distances between cultivated Manzanilla and wild type olives by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique. In this study, the cultivated type olive Manzanilla was supplied from Olive Research Institute of Turkey and the wild type olives from the villages of Manisa, Izmir and Mugla provinces. Genomic DNA's were extracted from young leaves and PCR was used to generate RAPD bands. Sixty random primers obtained from Operon technology were tested by RAPD-PCR (OP-A, OP-I, OP-Q). Forty one of 60 primers used in the study provided 346 considerably polymorphic bands and the average number of bands was defined as 8.43. Comparisons of cultivated and wild type olives are important for understanding genetic relation. It was determined that the genetic distance values varied between 0.0665 and 0.2863 and genetic similarity values varied between 0.9356 and 0.7511. It was observed that the samples most close to each other were wild 8 and wild 3 and the samples most distant from each other were wild 5 and Manzanilla, in accordance with such genetic distance and similarity values. © 2010 Academic Journals.