Browsing by Author "Yigitturk, G"

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    Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic rats
    Artunc-Ulkumen, B; Pala, HG; Pala, EE; Yavasoglu, A; Yigitturk, G; Erbas, O
    We aimed to evaluate: (1) endometrial and ovarian tissue injury caused by the glucose toxicity in diabetic rat model and (2) the effect of GLP-1 analog (exenatide) on endometrial and ovarian diabetes induced injury with emphasizing the underlying mechanism. The study group composed of 24 female rats assigned randomly into 3 groups. Group 1 was the control group (n = 8) and received no treatment. Diabetes was induced by intraperitoneal injection of streptozocin for 16 rats which are further assigned randomly into 2 groups: 1 ml/kg intraperitoneal saline was given to Group-2 (diabetic non-treated control group, 8 rats) and 10 mu g/kg/day of intraperitoneal exenatide was given to Group 3 (exenatide treated group, 8 rats) for four weeks. After four weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Diabetes caused endometrial and ovarian tissue injury in rats (p < 0.0001). Serum transforming growth factor beta (TGF-beta), malonylaldehyde (MDA), pentraxin-3 (PTX-3) levels were higher in diabetic rats (p < 0.0001), whereas antimullerian hormone (AMH) was lower (p < 0.001). Serum levels of these markers reflected that Diabetes induced injury in the reproductive tract occured via oxidative stress, fibrosis and severe inflammation. Diabetes diminished ovarian reserve. Exenatide treatment improved the histological degeneration and fibrosis in the endometrium and ovary with concomitant decrease in inflammatory and oxidative stress markers (p < 0.05). Exenatide also improved ovarian reserve (p < 0.05). Glucose toxicity occured severely in ovary and endometrium in DM. After exenatide treatment; ovarian and endometrial injury and fibrosis seems to decrease significantly. The effects of exenatide in rat models give hope to prevent the women with DM from premature ovarian failure and endometrial dysfunction.
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    HISTOLOGICAL EFFECTS OF POLLUTION ON GILL AND HEPATOPANCREAS TISSUES OF BLACK MUSSELS (M. GALLOPROVINCIALIS L.) FROM IZMIR BAY OF TURKEY
    Katalay, S; Yavasoglu, A; Yigitturk, G; Oltulu, F; Sari, G; Yavasoglu, NUK
    In the present study, the histological examination of the hepatopancreas (digestive gland) and gills of black mussels, Mytilus galloprovincialis was performed as a part of the biomonitoring programs carried out to assess the biological effects of the Izmir Bay (Western coast of Turkey). Mussels collected from five stations; Urla in the outer Bay and Inciralti, Goztepe, Konak, Pasaport in the inner Bay of Izmir Bay. While normal histology on hepatopancreas tissue was observed in Urla samples, vacuolar degenerations and hemocytic infiltrations were abundant in hepatopancreas throughout the other four stations. Also breakdown of digestive epithelium was determined in only Pasaport station. In gill tissues, while hemocytic infiltration was observed in Urla samples, gradually increasing cilia erosion, fusion, cell loss and necrosis was observed in Inciralti, Goztepe, Konak, Pasaport samples. Lumen was determined as normal in Urla and Goztepe samples and enlarged in Inciralti samples. The lumen in each filament was thinner and in some parts adjoined to each other and in Konak and Pasaport samples. Also the gills abfrontal tissue integrity was disturbed in these two stations.
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    Oxidative stress-induced apoptotic changes after acute exposure to antifouling agent zinc pyrithione (ZnPT) in Mytilus galloprovincialis Lamark (Mediterranean mussels) tissues
    Katalay, S; Guner, A; Dagdeviren, M; Yigitturk, G; Yavasoglu, A; Gunal, AC; Yavasoglu, NUK; Oltulu, F
    Zinc pyrithione (ZnPT) is one of the components used in antifouling paints and can be an alternative to classical toxic chemicals such as organotin. However, there is still remarkable concern about the environmental safeness of ZnPT due to rapid transchelation and degradation into several metabolites that have their own toxicity. The effect after acute exposure of ZnPT is investigated on Mediterranean mussels exposed to 20 and 40 mu g/L concentrations for 48 and 96 h and antioxidant responses [superoxide dismutase (SOD), and reduced glutathione (GSH)], genotoxicity [micronuclei (MN) frequency], apoptotic and histological changes were determined. Severe histological changes in hepatopancreas and gill tissues of mussels were observed in ZnPT exposed groups due to dose-dependent increase. ZnPT also caused a dose-dependent increase of TUNEL-positive cell count in the mussel tissues, especially in the hepatopancreas. Increasing in SOD activities and decreasing in GSH levels in both ZnPT concentrations compared to the control were observed. MN and binuclei numbers in all exposure groups were significantly increased. The results of the present study demonstrate that acute exposure to ZnPT could cause an adverse effect on mussel tissues at especially higher concentrations.
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    The Protective Effect of Losartan on Diabetic Neuropathy in a Diabetic Rat Model
    Cavusoglu, T; Karadeniz, T; Cagiltay, E; Karadeniz, M; Yigitturk, G; Acikgoz, E; Uyanikgil, Y; Ates, U; Tuglu, MI; Erbas, O
    Aim: Involvement of the peripheral and autonomic nervous systems is possibly the most frequent complication of diabetes. Important risk factors included hyperglycemia, dyslipidemia, hypertension, and smoking. Angiotensin-converting-enzyme inhibitor (ACE) inhibitors should be beneficial in all vascular beds, including neuropathy and retinopathy. In this study we aimed to evaluate the effect of the angiotensin receptor blocker losartan on diabetic neuropathy in a diabetic rat model. Material and Methods: 24 male, Sprague Dawley albino mature rats were divided into 3 groups; (1) control group: No drug was administered to the remainder of rats which blood glucose levels were under 120mg/dl, (2) diabetic control: rats were given no medication, but 4ml per day of tap water was given by oral gavage, (3) losartan groups: rats were given 10mg/kg/day oral of losartan for 4 weeks. Electromyography (EMG) was applied to anesthetized rats at the end of 4(th) weekend. Then, the animals were euthanized and sciatic nerve was performed for histopathological examination. Results: Compound Muscle Action Potential (CMAP) amplitude of diabetic rats receiving the Saline in the EMG was significantly reduced when compared to the control group. Distal latency value and CMAP duration of diabetic rats receiving the saline were meaningfully increased when compared to the control group. CMAP amplitude and CMAP duration of diabetic rats receiving the Losartan treatment in the EMG were meaningfully reduced when compared to diabetic rats receiving the Saline. Perineural thickness in the rats receiving the Losartan treatment was found to be significantly reduced when compared to the group receiving the Saline. Conclusions: As a result, it has been shown in this study that perineural thickness of the Losartan treatment was significantly reduced when compared to saline receiving group, significantly increased the immunoexpression of NGF, and also provided a significantly recovery in EMG when compared to Saline receiving group.