Browsing by Author "ZEKI ARI"

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    Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2-\r[P-bromophenyl]-benzoxazole in breast cancer cell lines
    (2022) Gorkem Kismali; Feyzan KURT; Mehmet İbrahim Tuğlu; ozlem temiz arpaci; Ercüment Ölmez; Mustafa Arısoy; FUNDA KOSOVA; ZEKI ARI
    We investigated the anticancer potential of 5-amino-2-[p-bromophenyl]-benzoxazole [BB] which is a new benzoxazole derivative in different breast cancer cell lines. BB\rwas applied to estrogen receptor positive [ER+] MCF-7 and receptor negative [ER-] MDA-MB cell lines and its effects were determined by histopathological examinations, viability test [MTT], immunocytochemistry assays [Tunnel, VEGF and eNOS]. Moreover, its effects on apoptozis-related proteins such as Apaf-1, cytochrome C,\rcaspase-3, bcl-2 and NF-κB were examined by western blot. Histopathological examinations showed that BB killed many cells for both cancer cell line while the cells lost\rtheir adhesion in MCF-7 and lost their adhesion and epiteloid morphology in MDA-MB. MTT analyses demonstrated that BB caused a clear dose depended toxic effect\rfor both cell types. BB application resulted in an increase labeled apoptotic cells after Tunnel staining which was more obvious for MDA-MB compared to that of MCF-7.\rVEGF staining was decreased after BB application in both cell lines. The decrease of VEGF staining was clearer for MDA-MB compared to that of MCF-7. The status of\roxidative stress was shown by eNOS immunocytochemistry which was increase after BB application in both cell lines. The levels of Apaf-1 and bcl-2 were found to be\rsimilar while caspase 9 and NF-κB levels were decreased compared to the control group after BB application for both cell lines. On the other hand, cytochrome C levels\rwere slightly increased in MCF-7 cells while this increase was found very obvious in MDA-MB cell lines in BB groups. In conclusion, BB which is a new benzoxazole\rderivative have shown significant anticancer effects by increasing apoptosis and decreasing angiogenesis in MCF-7 or MDA-MB breast cancer cell lines. These effects of\rBB seem to be more prominent for [ER-] MDA-MB cell lines which mimic more aggressive type of breast cancer. These results suggest that BB may be useful to treat\r[ER-] breast cancers and may be added to treatment protocols.