Browsing by Author "muhammet burak batir"

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    Recombinant Adeno-Associated Viral Vector Transduction of Human Prostate Cancer Cell Lines
    (2023) muhammet burak batir
    At the core of gene therapy lies the use of viral vectors, engineered viruses serving as delivery vehicles to transport restorative genes into target cells. Therefore, the effect of 7 different rAAV serotypes and their different quantites was analysis here on human prostate cancer cell lines PC-3 and DU-145, which are hard to be transfected. PC-3 and DU-145 cell lines were infected with different multiplicity of infection (MOI) ratios of 7 rAAV serotypes, which were expressing the green fluorescent protein (GFP) transgene driven by the CMV promoter. The transduction efficiency was analyzed by fluorescent microscopy and flow cytometry. In addition, the cell viability of the infected cells was measured by Muse Cell Analyzer at the MOI of 10.000. rAAV 2/2 and rAAV 2/6 have the most significant ability to transduce PC-3 cells. Although rAAV 2/2 and rAAV 2/6 were also the most transducing serotypes in the DU-145 cell line, the transduction rates did not exceed 20% in this cell line. On the other hand, after viral infection, no difference in cell viability was observed in PC-3 cells compared to the mock group, while a significant decrease in viability was observed in DU-145 cells. This study determined the transduction efficiency of 7 different rAAV serotypes on human cancer cell lines. While rAAV 2/2 and rAAV 2/6 serotypes achieved more than 60% transduction efficiency in PC-3 cells, the transduction efficiency could not exceed 20% in DU-145 cells. Overall, this study demonstrated that rAAV 2/2 and rAAV 2/6 could mediate the expression of a transgene with a high transduction efficiency
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    Allele and phenotype frequencies of CYP2D6 in the Turkish population
    (2024) safa can polat; muhammet burak batir; fethi sirri cam
    Aim: Cytochrome P450 is a hepatic enzyme system responsible for drug metabolism that comprises different iso-enzymes. The CYP2D6 enzyme, which composes 2-4% of all cytochrome enzymes, is responsible for 25% of the metabolisms of the drugs used in clinics. The genetic polymorphisms of this enzyme can change the efficiency and toxicity of the drugs used. In this study, the retrospective evaluation of CYP2D6 gene polymorphisms that influence enzyme activity in the Turkish population is targeted. Materials and Methods: From the 192 patients sent from psychiatry, medical genetics, and neurology polyclinics to our laboratory, we determined CYP2D6 enzyme gene polymorphisms by multiplex polymerase chain reaction (PCR) and multiplex allele-specific primer extension (ASPE). Results: Of all the cases, 82.29% were determined to be extensive, 12.5% intermediate, 2.08% poor, and 3.13% ultra-rapid metabolizers. In our population, the most frequent alleles were *1 (37.63%), *2 (24.75%), *41 (15.15%), *4 (9.85%), and *10 (4.29%), respectively. Conclusion: Compared to previous studies conducted on Turkish society, the percentage of extensive metabolizers was higher in the current examined population. In contrast, the percentages of poor and ultra-rapid metabolizers were lower. In addition, the *41 allele, which has not been studied before in our population, that follows the decrease in enzyme function was detected as the most frequent allele in this study. The fact that the percentage of the cases in which changes were observed was 17.1% will help determine the CYP2D6 gene mutations in the pre-treatment cases.
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    Erica arborea L. induces apoptosis and G2/M cell cycle arrest by regulating the CDK signaling pathway through the ROS generation in breast cancer cells
    (2024) muhammet burak batir
    Aim: The anticancer effect of any Erica L. genus member has not yet been investigated. Hence, this study investigated the anticancer effect of Erica arborea, a high-level flavanone producer, in mouse breast cancer 67NR and 4T1 cells. Materials and Methods: Healthy mouse fibroblast L929, mouse primary breast cancer 67NR and mouse metastatic breast cancer 4T1 cells were treated with Erica arborea methanol and ethanol extracts to investigate the cellular toxicity, apoptosis, gene expression level and oxidative stress effect on cells. Results: According to our data, the methanol and ethanol extracts of Erica arborea caused apoptosis and G2/M cell cycle arrest. Also, the methanol and ethanol extracts of Erica arborea enhanced the level of reactive oxygen species (ROS), especially higher levels in mouse breast cancer 67NR and 4T1 cells, according to the healthy mouse fibroblast L929 cells. The treatment of the cells with Erica arborea extracts causes up-regulation of the pro-apoptotic Caspase-3, Caspase-9, Bax, and Bak genes and down-regulation of the anti-apoptotic Bcl-2 and Bcl-xL genes. Moreover, treatment with Erica arborea extract up-regulated the expression levels of cyclin-dependent kinase (CDK) inhibitor P21 and P27 genes while down-regulating the expression levels of cyclin B1 and cyclin A genes in cells. However, the mRNA expression level changes of these genes are significantly higher in breast cancer 67NR and 4T1 cells, according to the healthy mouse fibroblast L929 cells. Conclusion: The result of the present study indicated that Erica arborea stimulated apoptosis induction in mouse breast cancer 67NR and 4T1 cells, which was associated with G2/M cell cycle arrest by regulating the CDK signaling pathway through ROS generation.