Browsing by Publisher "Aves Yayincilik"

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    Relationship between cyclic citrullinated peptide antibodies positivity and HLA-DRB1 shared epitope alleles in patients with rheumatoid arthritis in Turkey; [Türk romatoid artritli hastalarda siklik sitrüline peptit antikor pozitifliǧi ile HLA-DRB1 ortak epitop alelleri arasındaki i̇lişki]
    (Aves Yayincilik, 2010) Dayan I.; Tikiz C.; Taneli F.; Ulman C.; Ulutaş G.; Tüzün C.
    Objective: The most characteristic genetic risk factors for rheumatoid arthritis (RA), the HLA-DRB1 shared epitope (SE) alleles, encode for a common amino acid sequence in the peptide-presenting part of the HLA class II molecule. These SE alleles have been described recently to be a risk factor for the development of antibodies against citrullinated proteins in RA. The current study was performed to investigate the association between the cyclic citrullinated peptide antibodies (anti-CCP) and HLA-DR1 HLA-DRB1 shared epitope alleles in patients with RA in Turkey. Materials and Methods: Sixty patients with RA who were newly diagnosed or under conventional treatment in our clinic and 60 healthy volunteers as controls were enrolled in the study. In patients with RA anti-CCP levels were investigated with enzyme-linked immunosorbent assay and HLA-DRB1 subtyping and SE was assessed by polymerase chain reaction. Only anti-CCP was measured in healthy volunteers. Results: SE was positive in 50% of the patients with RA. Amongst the SE carriers, 30% of them were carrying double copy of SE. While anti-CCP was positive in 73,3% of patients with RA, this ratio was 0% in healthy volunteers. We determined that the existence of SE increases the positivity of anti-CCP (OR=4,3, 95% [CI], P=0.04), and a significant relationship was found between the anti-CCP positivity and the RF positivity. (OR=5,3, 95% [CI] P<0.05). Conclusion: The results of the present study revealed that Turkish patients with RA carrying SE with HLA-DRB1 genes is significantly related with the production of anti-CCP. The diagnostic sensitivity and specificity of anti-CCP for RA is determined as 73,3% and 100% respectively.
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    Levels of cytokines indicative of T cell response in bronchoalveolar lavage of tuberculin skin test-positive children; [Tüberkülin deri testi pozitif çocuklarda bronkoalveoler lavajda T hücre yani{dotless}ti{dotless}ni{dotless}n göstergesi sitokinlerin düzeyleri]
    (Aves Yayincilik, 2014) Yüksel H.; Yilmaz Ö.; Onur E.; Sürücüoǧlu S.; Erdin S.; Kirmaz C.
    Objectives: The aim of the study was to evaluate the levels of interleukin (IL)-4, IL-10, transforming growth factor-beta (TGF-βb), IL-17, and IL-23 cytokines, which reflect different T lymphocyte responses, in bronchoalveolar lavage (BAL) samples of tuberculin skin test (TST)-positive children. Material And Methods: Twelve children with TST positivity, who underwent flexible videobronchoscopy (FB) to evaluate airway involvement and to obtain BAL to improve diagnostic yield, and 11 control children with negative TST, who underwent FB for other reasons, were enrolled in this case-control study. BAL samples were obtained from all children during the FB procedure. Levels of IL-4, interferon gamma (IFN-γ), IL-10, TGF-βb, IL-17, and IL-23 were measured by the ELISA method. Results: Mean age of the children enrolled in the TST-positive and -negative groups were 8.6 (4.3) vs. 6.8 (4.5), respectively (p=0.35). There was a trend of higher TGF-βb levels in TST-positive children compared with TST-negative children [557.9 (515.3) vs. 386.3 (208.1), respectively, p=0.07]. Mean levels of IL-23 were 39.2 (29.5) in TST-positive children vs. 46.2 (72.3) in TST-negative children (p=0.49). IFN-γ, IL-4, IL-10, and IL-17 levels were not significantly different among the groups (p>0.05 for all). Conclusion: Results of this study suggest that TGF-in BAL fluid of children with TST positivity tends to be higher than that in TST-negative children, which indicates an increased activity of regulatory T lymphocytes that may decrease the Th1 immune response. TGF-might be studied in future research for its potential as a diagnostic modality and immunomodulatory treatment target. © 2014 by Turkish Thoracic Society.