Browsing by Publisher "Duzce University Medical School"

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    Sciatic nerve injury: Legal cases; [Siyatik sinir yaralanmasi: Adli olgular]
    (Duzce University Medical School, 2014) Şener M.T.; Kır M.Z.; Şahingöz S.; Ancı Y.; Kök A.N.
    Objective: Sciatic nerve (SN) injuries may occur as a result of traumatic events or in result of medical interventions during the injections. This study was carried out to determine the causes of sciatic nerve injury which was subject to judicial scrutiny. Method: 16,827 cases were analyzed between the years 2002 and 2011 which were sent to Department of Forensic Medicine of Atatürk University were evaluated retrospectively. A total of 21 SN injury cases were evaluated Results: The reason of SN injury were in 10 cases (47.6%) intramusculer (IM) injection. In EMG of these 10 cases (47.6%) were identified as tibial-peroneal nerve injury. Physical examinations showed foot drop in 7 cases (33.3%) and dorsiflexion or plantar flexion weakness in 8 cases (38.1%). Conclusion: More than half of the cases of SN injury were due to IM injection. For that reason, IM injection should be made by authorized medical staff and these staff should be well educated. © 2012 Düzce Medical Journal.
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    Induction of autophagic cell death by thymoquinone in docetaxel resistant prostate cancer cells; [Dosetaksel dirençli prostat kanseri hücrelerinde timokinon tarafından otofajik hücre ölümünün indüklenmesi]
    (Duzce University Medical School, 2021) İlhan S.; Oğuz F.
    Aim: Acquired docetaxel (DOC) resistance of prostate cancer (PCa) is still a clinical problem. In addition to failure in chemotherapy treatment, it causes tumor recurrence. Therefore, novel and more effective compounds are needed in DOC-resistant PCa treatment. This study aimed to investigate the possible cytotoxic and cell death-inducing activities of thymoquinone (TQ), one of the main active components of Nigella sativa L., on DOC-resistant prostate cancer cells. Material and Methods: DOC-resistant PC3 cells (DOC-R/PC3) were developed by the continuous culture with increment concentrations of DOC (1-10 nM) until they improved their growth and division abilities. The cell viability was determined by MTT assay. The Muse™ Annexin V & Dead Cell kit was performed to detect apoptotic cell death. Autophagic vacuoles were observed by staining autophagic vacuoles. The levels of LC3I, LC3II and Beclin-1 proteins were investigated via western blot analysis. Results: TQ inhibited the viability of DOC-R/PC3 cells in a dose-and time-dependent manner (p=0.014). The IC50 value of TQ for DOC-R/PC3 cells was calculated as 60 µM at 72 h. Treatment of TQ did not induce apoptotic cell death in DOC-resistant prostate cancer cells but induced the formation of autophagic vacuoles. Moreover, Beclin-1 and LC3-II protein levels were increased in TQ-treated DOC-R/PC3 cells, however, LC3-I levels were decreased in DOC-R/PC3 cells. Conclusion: All these results show that TQ may become a new therapeutic target for DOC-resistant prostate cancer in the future. © 2021, Duzce University Medical School. All rights reserved.