Browsing by Publisher "Fundacao de Pesquisas Cientificas de Ribeirao Preto"

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    RAPD assay of wild-type olives in Turkey
    (Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.
    Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59. The genetic distance values ranged from 0.1498 to 0.6845, and genetic similarity values varied from 0.8609 to 0.5043. We found that the closest samples based on their genetic distance and similarity values were from Harlak and Sabancilar; the most distant samples were from Bornova and Bademli, © FUNPEC-RP.
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    Determination of the genetic relationships between wild olive (Olea europaea oleaster) varieties grown in the aegean region
    (Fundacao de Pesquisas Cientificas de Ribeirao Preto, 2010) Sesli M.; Yeǧenoǧlu E.D.
    The RAPD technique was used for determining genetic differences between 12 wild-olive varieties grown in the Aegean provinces of Izmir, Mugla, and Manisa in Turkey. Wild olives obtained from the same provinces were included in the same plot. Twenty of 25 operon primers (OP-I 4, OP-I 14, OP-I 15, OP-I 16, OP-I 17, OP-Q1, OP-Q2, OP-Q3, OP-Q4, OP-Q11, OP-Q12, OP-Q13, OP-Q14, OP-Q15, OP-Q16, OP-Q17, OP-Q18, OP-Q19, OP-Q20, OP-F1, OP-F2, OP-F3, OP-F6, OP-F7, OP-F8) yielded bands. The differences between the varieties were determined based on their genetic similarities, using principal coordinate analysis; genetic distances were determined using neighbor-joining analysis. The varieties wild 7 and wild 12 had the lowest genetic similarity (0.97, Jaccard similarity index); they also had the greatest genetic distance between them (0.3606, Nei's genetic distance). It was concluded that the RAPD technique is adequate for the evaluation of genetic relationships among wild olives. Principal coordinate analysis and neighbor-joining analysis gave results that support the Genetic relationships between wild olive varietie use of this type of analysis to help understand the genetic background of olives and for further genetic studies. ©FUNPEC-RP.