Browsing by Publisher "Journal of Clinical and Analytical Medicine"

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    The effect of erythropoietin on S100 protein expression in cochlea after acoustic overstimulation: An experimental study; [Eritropoetinin gürültü uygulaması sonrası kokleada s100 proteini ekspresyonuna etkisi: Deneysel Çalışma]
    (Journal of Clinical and Analytical Medicine, 2015) Gürgen S.G.; Gürgen O.; Kırkım G.; Kolatan H.E.; Gürkan S.; Eskiizmir G.
    Aim: To investigate the effect of Erythropoietin on acoustically overstimu-lated rat spiral ganglion neurons (SGNs) using S100 protein immunostaining. Material and Method: Twenty-two Wistar albino rats were divided into three groups: healthy control group (n=7), Saline solution (n=7) and Erythropoietin injection groups (n=8). Saline solution and Erythropoietin injection groups re-ceived white noise (100 dB SPL) for 3 hours. Cochlear sections were stained by silver staining technique and immunostained by S100 antibody. Results: Histochemical analysis of silver staining sections revealed normal structure and a weak staining in SGNs of healthy control group. However, dark-black cytoplasmic staining, cellular shrinkage and degeneration were detected in saline injection group. On the other hand, a few weakly stained neurons were observed in erythropoietin injection group. S100 staining demonstrated strong reaction in Schwann cells and myelin sheaths of SGNs in healthy con-trol group (p<0.05). In saline solution injection group, Schwann cells showed moderate S100 reaction and other regions of SGNs showed weak reaction (p<0.05). In erythropoietin injection group, strong S100 expression almost similar to the healthy control group was determined, although there was an occasional decrease. Discussion: Erythropoetin may prevent noise induced SGN degeneration via protecting the Schwann cells in rat cochlea. © 2015, Journal of Clinical and Analytical Medicine. All rights reserved.
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    Is melatonin protective in contrast material related renal failure?; [Melatonin kontrast madde ile ilişkili renal yetmezlikten korur mu?]
    (Journal of Clinical and Analytical Medicine, 2015) Tuncyurek O.; Gunay O.; Taneli F.; Ayhan S.; Nese N.; Pabuscu Y.
    Aim: The aim of the study was to investigate the effect of melatonin on the renal injury resulting from radiocontrast media and myoglobinuria in male Wistar albino rats. Material and Method: 50% glycerol at equal amounts was intramuscularly administered to both hind legs of all animals under ether anesthesia at the dose of 10 mg/kg. Three hours later, the groups were ad-ministered the following: Group I (number:7): Iopromide(Ultravist -300®) at the dose of 2 ml/kg (intracardiac); Group II (number:7): Iopromide(Ultravist -300®) and intraperitoneally administered Melatonin at the dose of 10 mg/kg (Melatonin was dissolved in 7.5% absolute ethanol and further dilutions were made in saline.); and Group III (number:7): 2 ml/kg of sterile physiologic saline (Control group). The levels of Uurea, Ccreatinine and Ccystatin C were studied on the blood samples collected. The renal samples were evaluated by 2two distinct pathologists who did not know the protocol. Results: There was no difference in the values of Creatinine and cystatin c between Groups 2 and 3 (p=0.9; 0.2). Discussion: In conclusion, we evaluated the possible prevention of contrast-induced oxidative stress in the kidney with using melatonin. How-ever, additional clinical studies are needed to evaluate the role of preventive melatonin treatment in humans. © 2015, Journal of Clinical and Analytical Medicine. All rights reserved.