Browsing by Publisher "KAFKAS UNIVERSITY"

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    The effect of tunicamycin on embryonic and newborn murine spleen tissues; [Tunikamisinin embriyonik ve yenidoǧan fare dalak dokularına etkisi]
    (KAFKAS UNIVERSITY, 2009) Balcan E.; Arslan Ö.; Gümüş A.; Şahin M.
    Tunicamycin is an antibiotic that widely used in cell biology for its ability to inhibit N-linked glycosylation of asparagine residues on proteins and to induce endoplasmic reticulum stress. In the present study, the effects of tunicamycin on murine splenic tissues at 17th embryonic day and 1st and 3rd postnatal days were evaluated with three structural and physiological parameters: 1) alterations in glycosaminoglycan molecules, 2) apoptosis and 3) alterations in laminin molecules. It was shown that splenic microenvironments of control groups contain carboxylated glycosaminoglycans, but their content slightly decreased in all tunicamycin treated groups by alcian blue-periodic acid-Schiff method. On the other hand, there was an increase in the interstitial space among the cells of tunicamycin treated groups. In addition, it was shown by immunoblotting analyses that expression levels of laminin molecules were decreased by tunicamycin tratment in developing spleen tissues. In order to determine apoptotic effects of tunicamycin, TUNEL assay was performed and it was found that the number of apoptotic cells in developing spleen was strongly increased with tunicamycin treatments. These results suggest that, during the spleen development, the alterations of glycosaminoglycan contents in the extracellular matrix and the glycosylation status of extracellular glycoproteins (e.g. laminins) that mediate cell-extracellular matrix interactions are very important factors that seal the fate of cell physiology and morphogenesis.
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    Inhibitory effects of propolis on human osteogenic sarcoma cell proliferation mediated by caspase patway; [Kaspaz yolaǧı ile yönlendirilen i̇nsan osteojenik sarcoma hücre çoǧalmasına propolisin baskılayıcı etkisi]
    (KAFKAS UNIVERSITY, 2010) Ozdal Kurt F.; Vatansever H.S.; Sorkun K.; Deliloǧlu Gurhan S.I.; Turkoz E.; Gencay O.; Salih B.
    A natural product Propolis, is a resinous material gathered by honeybees from the buds and bark of certain trees and plants. Propolis contains various chemical components of biological activities, including antimutagenic, antioxidant, antibacterial, antiviral and anticarsinogenic. Therefore, the aim of this study is to investigate the antiapoptotic effect of propolis extracts (PE) using caspase pathway in the human osteogenic sarcoma cell line SAOS-2 in culture. The extracts which produced in ecologic environment were taken from the Hacettepe University, Beytepe Campus area-Ankara were used. Seven different PE at 0.5, 0.25, 0.125 and 0.063 mg/ml were added to SAOS-2 cell line for two days incubation. For cell proliferation and cytotoxicty analyses MTT, for apoptotic cell death determination TUNEL method, for distribution of caspase 6, caspase 8 and caspase 9 indirect immunocytochemistry analyses were used. After MTT analyses, the most effect was observed PE 7 at the 0.125 mg/ml dilution. The number of TUNEL positive cells was more detectable at PE 4 and 5 at the 0.063 mg/ml, and PE 7 at the 0.125 mg/ml dilutions. The immunoreactivity of caspase 6 was stronger than caspase 8 and 9. Moreover, density of caspase 6 staining was much better especially in PE 7 at the 0.125 mg/ml dilution. In conclusion, the mechanisms of apoptosis induction by PE may appear via caspase pathway because of its anticanserogenic effect. PE may be usefull in the cancer treatment protocol.