Browsing by Publisher "Pleiades journals"
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Item The Experience in Lectins Application to Assess Changes in the Carbohydrate Composition of Murine Thymocytes Glycocalyx in the Early and Late Apoptotic Stages(Pleiades journals, 2020) Serebriakova M.K.; Kudryavtsev I.V.; Balcan E.; Polevshchikov A.V.Abstract: The apoptotic process is an important step in the process of T-lymphocytes maturation and differentiation. This work is devoted to the analysis of changes in the composition of oligosaccharides of CBA mice thymocytes glycocalyx during the hydrocortisone induced apoptosis. A panel of 23 fluorescein isothiocyanate (FITC)-labeled lectins specific for mannose, mannose and glucose, galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, fucose and N-acetylneuraminic acid residues was used. Flow cytometry was used for evaluation the binding of lectins to thymocytes of intact mice, as well as mice after administration of hydrocortisone. Based on the results of TMRM and 7AAD staining, the cells were divided into living thymocytes, cells in early and late apoptosis. It has been established that living cells carry carbohydrates on the surface of glycocalyx containing terminal residues of galactose and N-acetyl-D-galactosamine. With the transition of thymocytes to late apoptosis, the binding of all lectins increases, except for fucose-specific ones. The glycocalyx structures of living thymocytes are low in density and contain groups of oligosaccharides with N-acetyl-D-galactosamine and D-galactose in the terminal position. The membrane layer of glycocalyx is characterized by a high density and a wide variety of oligosaccharide structures that persist at the stage of late apoptosis of thymocytes. The results indicate nonuniform density and heterogeneity of oligosaccharides in glycocalyx, a significant part of which is lost in early apoptosis. © 2020, Pleiades Publishing, Ltd.Item Effects of ECAP with One Pass Severe Deformation on Microhardness, Texture, and Corrosion Behavior of AA1370 Aluminum Alloy Wire(Pleiades journals, 2021) Youcef Hadj Ali; Zazi N.; Durmus H.; Chopart J.-P.Abstract: In this paper, We investigate the effects of one pass severe deformation, obtained by equal channel angular pressing (ECAP) process, using dies with channel angles of 90° and 120°, on hardness, texture, and corrosion behavior, of AA1370 aluminum alloy wire. A severe deformation, by ECAP process, leads to athinning of several diffraction peaks and an increase of their angular values; modifies the texture of material from pseudo-texture to mix texture; reduces the microhardness; modifies a part of localized corrosion towards generalized one; shifts the corrosion potential to negative values; and increases the values of open circuit potential (OCP). The deformation by ECAP process induces fragmentation of parts of intermetallic particles, and reduction of grains size. The imprisoned air, between the channel wall of the die and the wire, creates microporosities in the deformed samples. © 2020, Pleiades Publishing, Ltd.Item Preliminary Cesium Adsorption Study with a Unique Iron(III) Ferrocyanide/Vermiculite Nanocomposite via One-Pot Hydrothermal Synthesis(Pleiades journals, 2022) Akalın H.A.; Hiçsönmez Ü.Abstract: A novel iron(III) ferrocyanide (Prussian blue)/vermiculite nanocomposite was synthesized, characterized and the cesium removal efficiency of the nanoadsorbent was investigated in this study. Characterization studies revealed that the nanocomposite was successfully produced and the saturation magnetization value was found as 0.15 emu/g. Low magnetization value was due to the low amount of Fe3O4 (magnetite). Adsorption isotherm studies confirmed that the process was in accordance with Langmuir, Freundlich, and D‒R (Dubinin–Radushkevich) isotherm models, suggesting that the reaction mechanism was both monolayer and multilayer, also had a physical sorption character with 0.0906 kJ/mol adsorption energy. Higher linear regression values obtained from Langmuir and D–R isotherms proved that chemical adsorption mechanism was more favorable than physical adsorption and the nanoadsorbent had 49.50 mg/g maximum adsorption capacity. © 2022, Pleiades Publishing, Ltd.Item Age of Information Minimization for Energy Harvesting Overlay/Underlay Cognitive Radio Networks(Pleiades journals, 2022) Karaca H.M.Abstract: Cognitive radio networks (CRNs) having energy harvesting capability have been an encouraging technology to solve the spectrum deficiency problem in wireless networks. Nevertheless, the wireless spectrum must be correctly administered to achieve the strict needs on integrity and transmission of information with minimum delay. Influenced by this, this paper takes into account the measure of the Age of information (AoI) to serve as the newness of data in the multi-channel (m-channel) allocation problem of hybrid CRNs. Here, energy can be harvested by secondary transmitters (STs) to improve channel distribution performance. Two new mechanisms have been proposed to limit average AoI values whilst assigning channels to STs by satisfying energy and collusion constraints. A new measure is proposed to assign precedence to STs, and they are permitted to utilize channels only if the resultant AoI value fulfills presented conditions. AoI levels are computed based on cochannel interference between STs and minimized by reducing interference levels. For comparison, three algorithms were studied: a greedy mechanism for m-channel allocation of hybrid CRNs without AoI control, and the presented m-channel distribution schemes based on ranking STs according to the presented measure and limiting AoI levels based on cochannel interference. The simulations highlight that the performance of the presented m-channel allocation schemes outweighs the greedy scheme in terms of both AoI minimization and maximization of channel allocation performance which demonstrates the primacy of the proposed algorithms. © 2022, Pleiades Publishing, Ltd.Item The Effect of Nitric Oxide Synthase Inhibition in Developing Chick Embryo Lungs(Pleiades journals, 2022) Esra Ucar; Çöllü F.; Gürcü B.Abstract: We identified the developmental stages of the chicken lung, and explored the histopathological effects of L-nitro-arginine-methyl-ester (L-NAME) on such development at different times. L-NAME was injected into the vitellus, at two different doses, on the day of embryogenesis 6, 8, 9, 11, 13, and 16. Sham-injected and experimental embryos were collected on the day of incubation 7, 9, 10, 12, 14, and 17. Chicken lungs developed in four stages: embryonic (4–7 days), pseudoglandular (8–14 days), canalicular (14–17 days), and saccular (or alveolar) (day 17 and postnatally). Most differentiation occurred in the pseudoglandular stage. L-NAME triggered mesenchymal tissue loss, reduced airway branching and lung volume, narrowed bronchial diameters, triggered formation of pulmonary emboli, enhanced alveolar protein accumulation, caused regional bleeding (hemorrhage), triggered abnormal blood vessel modeling, and reduced vessel diameter. Sham-injected embryos exhibited strong immunoreactivities against endothelial and inducible nitrous oxide synthase in the embryonic and canalicular stages; immunoreactivities were reduced at all developmental stages in the experimental group. © 2022, Pleiades Publishing, Ltd.