Browsing by Publisher "Science Printers and Publishers Inc."

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    Effect of erythropoietin on acoustically traumatized rat cochlea an immunohistochemical study
    (Science Printers and Publishers Inc., 2014) Gürgen O.; Gürgen S.G.; Kirkim G.; Kolatan E.; Gürkan S.; Güvenç Y.; Eskiizmir G.
    Objective: To investigate the audiological and histopathological effects of erythropoietin on acoustic overstimulation in rats. Study design: Twenty-two male Wistar albino rats were divided into 3 groups: sham group (n=7), erythropoietin injection group (n = 8), and saline injection group (n=7). Both erythropoietin and saline injection groups were exposed to white noise (100 decibel [dB] sound pressure level [SPL]) for 3 hours. Auditory brainstem responses were measured before, immediately after, and on the 7th day of noise exposure. All animals were sacrificed on the 7th day and temporal bones were collected. The serial sections of the cochleae were stained by caspase-3 and caspase-9 immunostaining and by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method in order to detect apoptotic cells. Results: In the saline group statistically significant differences were detected between the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli. However, when the baseline and immediate postacoustic overstimulation thresholds of click and 6 kHz stimuli were compared in the erythropoietin injection group, no statistically significant difference was determined. Histopathologic evaluations demonstrated that erythropoietin decreased the amount of apoptotic cells in the cochlea. Conclusion: Erythropoietin is likely to prevent the acute threshold changes and decrease the amount of apoptosis in cochlea after acoustic overstimulation in rats. © Science Printers and Publishers, Inc.
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    Distribution of furin, TNF-α, and TGF-β2 in the endometrium of missed abortion and voluntary first trimester termination case
    (Science Printers and Publishers Inc., 2015) Ozbilgin K.; Turan A.; Kahraman B.; Atay C.; Vatansever S.; Uluer E.T.; Özçakir T.
    Objective: To identify the role of furin, TNF-a, and TGF-b2 in human missed abortion pathogenesis. STUDY DESIGN: Decidual materials were collected from patients diagnosed with a missed abortion (n=10) (missed abortion group) and from legal voluntary termination cases at ≪ 10 gestational weeks (n=10) (normal pregnancy group). Tissue samples were collected from each group by dilation and curettage under mask anesthesia. For all tissue samples, furin, TNF-α, and TGF-β2 primary antibodies were performed by immunohistochemical staining. The number of stained cells was evaluated by using the H-score technique. Results: In immunohistochemical examination, the immunoreactivities of furin, TNF-α, and TGF-β2 were found to be higher in syncytiotrophoblastic cells in the missed abortion group than in the normal pregnancy group (p<0.005). Additionally, high immunoreactivity of TNF-α and TGF-β2 molecules was established only in cytotrophoblastic cells of missed abortions (p<0.005) in examination at decidual cells of the missed abortion group; furin immunoreactivities were detected higher in the missed abortion group than in the control group, but TNF-α and TGF-β2 immunoreactivity were increased in number in the normal pregnancy group (p<0.005). Conclusion: It is considered that high levels of furin and the 2 furin-related proteins (TNF-α and TGF-β2), which play important roles in proliferation, invasion, migration, differentiation, and survival of cells, may be the reason of proceeding decidualization, placentation, and prevention from abortion, in spite of terminating the fetal life. © Science Printers and Publishers, Inc.
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    Effect of tunicamycin on glycosaminoglycans and laminins in embryonic and postnatal thymic tissues
    (Science Printers and Publishers Inc., 2015) Balcan E.; Arslan Ö.
    OBJECTIVE: To compare histological and molecular alterations in the embryonic and neonatal thymi following exposure to tunicamycin. STUDY DESIGN: Mouse embryos at gestational days 17 (n=7) and 18 (n=7) and newborn animals at postnatal days 1 (n=5) and 3 (n=5) were divided into two groups: control and tunicamycin-treated. Combined Alcian blue and Periodic acid-Schiffsequences immunohistochemistry and immunoblotting were performed to determine glycosaminoglycan (GAG) accumulation and laminin expression in control and tunicamycin-treated embryonic and postnatal thymi. The apoptotic effect of tunicamycin was evaluated by TUNEL assay. RESULTS: In the control group acidic GAGs first appeared in medullary cells at postnatal day 3, whereas treatment with tunicamycin promoted the accumulation of acidic GAGs in all treated groups as of embryonic day 17. However, tunicamycin slightly decreased the laminin expression, and the number of apoptotic cells was considerably increased after tunicamycin treatment. CONCLUSION: Results suggest that carboxylated and acidic GAGs are two presumptive candidates to establish the thymic microenvironment during the late fetal development and postnatal periods of mice and that tunicamycin would be implicated in this establishment by increasing the acidic GAG accumulation and by reducing the laminin expression and the thymic stromal cell population. © Science Printers and Publishers, Inc.
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    Effects of cyclooxygenase on the urothelium of the urinary bladder of mice exposed to pelvic radiation
    (Science Printers and Publishers Inc., 2016) Ozbilgin M.K.; Onal T.; Ozcan C.; Temel M.; Aktas C.; Gareveran M.S.; Uluer E.T.; Inan S.; Kurtman C.
    OBJECTIVE: To determine the role of cyclooxygenase (COX) expression in the urothelium of the urinary bladder during radiation injury caused by pelvic radiotherapy for cancer therapy. STUDY DESIGN: Twenty-four male Swiss Albino mice were separated into 4 groups. The first group was the control group (Group 1) and the second, third, and fourth groups were euthanized after 24 hours (Group 2), 48 hours (Group 3), and 7 days (Group 4), respectively. A single-fractioned 10 Gy of ionizing radiation was applied to all mice's pelvic zone with Co-60. Bladders were removed completely from the pelvic region. Histochemical analysis using hematoxylin and eosin and immunohistochemical analysis using anti-COX-1 and COX-2 antibodies were performed on tissue samples. The immunoreactivities of the urinary bladder were quantified using H-score measurement, and statistical comparison was performed. RESULTS: In the immunohistochemical examination the COX-1 immunoreactivities were found to be higher in the urothelium of the bladder in the radiation ex-posed groups than in the normal control group (group 1) (p<0.005). Additionally, high immunoreactivity of COX-2 molecule was established in groups 2, 3, and 4 of radiation groups as compared to group 1 (p<0.005) in examination of the urothelium. COX-1 and COX-2 immunoreactivities in the submucosa were detected higher in group 4 than in the other groups (p<0.005). CONCLUSION: COX-1 and COX-2 expressions in the urothelium and subepithelium of the urinary bladder were investigated in mice during the acute radiation response. The expression of COX-1 and COX-2 in the urothelium seems to prevent bladder damage from radi-ation, supplying differentiation and restoration of the urothelium. © Science Printers and Publishers, Inc.
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    Influence of radiation exposure during radiotherapy: Evidence for the increase of versican and heparin-binding EGF-like growth factor concentrations
    (Science Printers and Publishers Inc., 2016) Ozbilgin M.K.; Aktas C.; Uluer E.T.; Buyukuysal M.C.; Gareveran M.S.; Kurtman C.
    OBJECTIVE: To investigate the reaction of versican and heparin-binding EGF-like growth factor (HB-EGF) molecule concentrations to acute radiation exposure in normal bladder and rectal tissue samples in order to gain more insight into the effects of cancer radiotherapy. STUDY DESIGN: Four groups with 6 male adult Swiss Albino mice per group were investigated. The mice bladder and rectum tissue samples were subjected to a 10-Gy single-dose radiation exposure in the pelvic region with a Co-60 teletherapy device and investigated 1, 2, and 7 days after radiation exposure, with 1 reference group which was not exposed to radiation. RESULTS: In the immunohistochemical examination of the tissue samples with anti-versican and anti-HB-EGF primary antibodies was observed a statistically significant increase 7 days after radiation exposure. CONCLUSION: The observed increase of versican and HB-EGF concentrations in the normal tissue matrix after radiation exposure may play a role in the side effects of radiotherapy. © Science Printers and Publishers, Inc.