Browsing by Publisher "University of Ankara"

Now showing 1 - 4 of 4
  • No Thumbnail Available
    Item
    Determination of cocaine in urine by GC/MS method; [GC/MS yöntemi̇ i̇le i̇drarda kokai̇n tayi̇ni̇]
    (University of Ankara, 2004) Cengiz G.; Çeçen Ş.Ş.; Söylemezoǧlu T.
    Although there are not enough researches in our country about drug abuse depending upon toxicological analysis, there is an impression that cocaine abuse is becoming widespread from current data. As a result, appropriate analytical toxicological method should be established in order to determine the drug abuse. Cocaine is a natural alkaloid that is toxic to central nervous system, heart, liver, neuromuscular system and kidneys. Widespread abuse of cocaine is an important health problem in society. Cocaine is usually misused with ethanol, opioids, marihuana and sedative hypnotics. In our study, qualitative and quantitative methods are established to determine cocaine levels from urine by GC/MS (gas chromatography mass spectrometry). Precision, accuracy, specificity of the established method is evaluated. In order to isolate cocaine from urine, liquid-liquid and solid phase extraction methods are applied and compared. Recovery rates of 77.6% by liquid-liquid extraction method and 81.2% by SPE method were obtained. In one of the suspicious urine samples, morphine is determined besides methylecgonine and benzoylecgonine.
  • No Thumbnail Available
    Item
    Micronucleus levels and sister chromatide exchange frequencies in boron exposed and control individuals; [Bora maruz kalan bi̇reylerde ve kontrol bi̇reyleri̇nde mi̇kroçeki̇rdek düzeyleri̇ ve kardeş kromati̇d deǧi̇şi̇m frekanslari]
    (University of Ankara, 2009) Uçkun Z.; Us̈tündaǧ A.; Korkmaz M.; Duydu Y.
    The aim of the study was to investigate the genotoxic effects in boron exposed humans. SCE frequencies in boron exposed and control group were 7,28 ± 1,60 and 9,50 ± 1,83, respectively. SCE frequencies in boron exposed individuals were significantly lower than the controls (Mann Whitney U- test, p<0,05). The MN values in boron exposed individuals were also lower than the control group. Nevertheless the difference between boron exposed humans and controls was statistically not significant (χ2 test, p>0,05). In spite of the statistical insignificancy, lower MN values were also observed in boron exposed group. When these results were taken into consideration the decrease in SCE and MN values might be a reflection of a protective effect of boron agonist DNA damage. However this suggestion surely needs further investigation.
  • No Thumbnail Available
    Item
    Apoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells; [ORIGANUM MINUTIFLORUM'UN NB2A NÖROBLASTOMA HÜCRELERINE APOPTOTIK VE SITOTOKSIK ETKISI]
    (University of Ankara, 2020) Sari I.; Donmez Arat G.; Ozkan O.; Aydemir I.; Ozbey C.; Tuǧlu M.I.
    Objective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells. Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively. Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50doses were found to be10.75 μl/ml for oregano oil and 5μM for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels. © 2020 University of Ankara. All rights reserved.
  • No Thumbnail Available
    Item
    The investigation of antiproliferative effects of quercetin, curcumin and their combinations on breast cancer cell lines; [Kuersetin, kurkumin ve kombinasyonlarinin meme kanseri hücre hatlari üzerindeki antiproliferatif etkilerinin arastirilmasi]
    (University of Ankara, 2020) Altundag E.M.; Becer E.; Gencąlp D.; Vatansever H.S.
    Objective: Breast cancer is a type of cancer that has high malignancy and mortality rates in women and is frequently seen in the Eastern Mediterranean region. Flavonoids such as quercetin and curcumin are known to have an ability to inhibit the growth of cancer cells. The aim of this study was to determine the antiproliferative effect of quercetin and curcumin which are flavonoids known to have anticancer properties on human metastatic and non metastatic breast cancer cell lines of alone and in combination usage. Material and Method: Cell growth and cytotoxicity of quercetin, curcumin and quercetin:curcumin combinations were measured with MTT assay both on M4A4 and MCF-7 breast cancer cell lines. Combosyn program was used to determine IC50 and combination index (CI) values. Result and Discussion: Half-maximal inhibitory concentrations (IC50) of quercetin for 72 hours were 49 μM and 120 μM on M4A4 and MCF-7 cells, respectively. IC50 doses for curcumin on M4A4 and MCF-7 cells were determined as 27 μM and 54 μM, respectively. It was determined that quercetin:curcumin combinations applied were shown a synergistic and antagonistic effect on MCF-7 and M4A4 cells. It was found that both quercetin and curcumin treatment inhibit cell viability in a dose and time-dependent manner. Findings show that combination with quercetin and curcumin polyphenol can be used in non-metastatic breast cancer, while different combination studies are needed for metastatic breast cancer. © 2020 University of Ankara. All rights reserved.