Browsing by Subject "CARCINOGENESIS"

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    Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk
    (AVES) Bilgiç, F; Gerçeker, E; Boyacioglu, SÖ; Kasap, E; Demirci, U; Yildinm, H; Baykan, AR; Yüceyar, H
    Background/Aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-gPCR primer assay data analysis software. Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EEO, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup. Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG.
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    Evaluation of Phytic Acid Content of Some Tea and Nut Products by Reverse-Phase High Performance Liquid Chromatography/Visible Detector
    (SPRINGER) Dost, K; Karaca, G
    Phytic acid contents of nine different types of nut and six different types of tea and steeped tea were analysed by reverse-phase high performance liquid chromatography with visible detector. The extraction method was based on hydrochloric acid extraction, and the analysis method was based on metal replacement reaction of phytic acid from coloured complex (iron(III)-thiocyanate), separation on CN column and monitoring the absorbance at 460 nm. The retention time for the monitored iron(III)-thiocyanate peak was achieved less than 3 min. The proposed HPLC/Vis procedure shows good linearity over the concentration range of 1-150 mg L-1 with a correlation coefficient value of 0.9938. The effectiveness of metal replacement reaction was presented in terms of relative standard deviation that was 0.62 and 0.88 % for 5 and 50 mg L-1 of phytic acid, respectively. Repeatability of the analytical method was ranging between 1.58-7.88 % (n = 10, for 50 mg L-1) and 0.98-4.63 % (n = 10, for 5 mg L-1) in terms of relative standard deviation. Accuracy of the method is good, ranging relative error between 4.52 and 8.00 % (n = 10, for 50 mg L-1). Phytic acid content is in the range of 1.54 to 9.74 mg g(-1) in nuts, 27.67 to 28.82 mg g(-1) in green teas, and 20.49 to 21.96 mg g(-1) in pocketed roasted teas.