Browsing by Subject "EPITHELIAL-MESENCHYMAL TRANSITION"

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    Anti-Tumorigenic Effect of Resveratrol in HepG2 cells Controlled with Cytochrome-c Dependent Cell Death
    (MATTIOLI 1885) Kiyak, N; Becer, E; Vatansever, HS; Kükner, A
    Resveratrol is a phytochemical that is regarded as a potential anticancer agent in liver cancer prevention. It had also been shown that resveratrol has role in the prevention of cancer and anti-cancer properties. In this study, we aimed to investigate the effects of resveratrol on cell viability, apoptosis, cellular proliferation, JAK/STAT pathway and epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cell (HepG2)line. Cell growth and cytotoxicity were evaluated with MTT assay with different concentrations (5, 10, 25, 50, 100 mu M) of resveratrol in HepG2 cells. The distribution of FasL, cyt-c, caspase-3, Ki-67, ACTA2, CD133, JAK2, N-cadherin, vimentin and STAT3 in HepG2 cells were analyzed using indirect immunoperoxidase technique. The effective dose and incubation time for inhibition of cell growth in HepG2 cells was determined as 100 mu M for 48 hours. Decreased Ki-67 immunoreactivity following resveratrol application was significant in HepG2 cells. Increased cytc-c, STAT3, vimentin, N-cadherin and CD133 immunoreactivities were significant between resveratrol appli-cated HepG2 cells and control group. According to our results, resveratrol induced mitochondrial-dependent cell death and suppressed proliferation in HepG2 cells. On the other hand, our results showed that resveratrol stimu-lated cellular self-protection responses through activation of EMT and STAT3 protein expression in HepG2 cells