Browsing by Subject "EXTRACELLULAR VESICLES EXOSOMES"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    The correlation between unexplained infertility and exosomes
    (VIA MEDICA) Uyar, Y; Özgül, M; Gökalp, S; Ok, G; Tan, A; Vatansever, HS
    Objectives: Endometrial receptivity plays the most important role for successful implantation. Increasing endometrial receptivity may improve infertility and increase Assisted Reproductive Technologies success. The aim of this study was to investigate the effect of exosome specific markers CD63 and CD9 which are promising molecules in the pathogenesis and treatment of many diseases on endometrial receptivity in women with unexplained infertility. Material and methods: This prospective study was conducted between November 2015 and March 2017. Proliferation and secretion periods of endometrial samples from fertile and infertile cases were collected. The paraffin-embedded tissue sections were stained with hematoxylin-eosin for the immunohistochemical analysis distributions of CD63 and CD9. Results: The results of this study demonstrated that the CD63 immunoreactivity was higher in both luminal and glandular epithelium of infertile patients when compared with fertile patients during the proliferative phase (p = 0.009, p = 0.008). In the infertile proliferation phase, endometrium CD9 immunoreactivity was rarely detected in both the luminal and glandular epithelium. In the secretion phase of endometrium, CD9 immunoreactivity was mild in fertile patients, the increased immunoreactivity of CD9 was observed in both luminal and glandular epithelium of infertile patients (p = 0.037, p = 0.037). Conclusions: Increased levels of CD63 in infertile proliferation phase endometrium should represent an unfavorable signaling. Moreover, the increased levels of CD9 in infertile secretion phase endometrium could be used as a biomarker to evaluate endometrial receptivity. These exosome-specific markers can be considered as potential molecular markers of infertility.
  • No Thumbnail Available
    Item
    Direct impedimetric detection of exosomes and practical application in urine
    (SPRINGER) Sazaklioglu, SA; Torul, H; Vatansever, HS; Tamer, U; Çelikkan, H
    Exosomes are nanoscale vesicles released from tumor-derived cells. They are very popular cancer biomarkers in non-invasive liquid biopsy diagnosis and evaluation of therapeutic response. Rapid analysis of exosomes with proteins or other biomarkers requires complex methods and has a relatively high cost. We indicate the fabrication of a biosensor for the detection of exosomes at low concentrations using immunological recognition strategies. The implementation of the biosensor consists of two steps: (i) functionalization of screen-printed electrodes (SPEs) with tetraspanin anti-CD63 antibodies and (ii) capturing exosomes by the electrode surface. Label-free detection of exosomes was successful with the Nyquist diagram, one of the main demonstrations of electrochemical impedance spectroscopy (EIS). In addition, we increased the consistency of the results by including the Bode diagrams of the measurements in our study. The proposed biosensor as a sensitive and reliable impedimetric method for detecting exosomes was established in a working range of 3.7 x 10( )3 to 3.7 x 10(8) particles mL(-1) with a detection limit of 2.0 x 10(3) particles mL(-1). [GRAPHICS] .