Browsing by Subject "Eosinophils"
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Item Correlation of quality of life with clinical parameters and eosinophilic cation protein levels in children with allergic rhinoconjunctivitis(2008) Yuksel H.; Yilmaz O.; Sogut A.; Ertan P.; Onur E.Background: Systemic and mucosal roles of eosinophils in the pathogenesis of allergic rhinoconjunctivitis (AR) are known. The aim of this study was to investigate the relationship of clinical parameters and quality of life with eosinophilic cationic protein (ECP) in grass pollen-sensitive children with seasonal AR. Methods: This study included 31 children with AR and 18 healthy controls aged between 5 and 15 years. ECP levels in nasal lavage fluid and serum were measured. AR symptom scores were calculated and the pediatric rhinoconjunctivitis quality of life questionnaire (PRQLQ) was filled in for all patients. Results: Mean serum and nasal ECP levels (ng/ml) were significantly higher in the patient group (p < 0.05 for both). In the AR group, the mean clinical symptom score was 7.3 ± 2.2, while the total PRQLQ score, activity limitation, symptoms and emotional function domains were 2.5 ± 0.9, 3.0 ± 1.1, 2.5 ± 1.2 and 2.6 ± 1.1, respectively. The total clinical symptom score and disease duration showed a significant correlation with the total PRQLQ score (p = 0.00 and 0.003, respectively). However, neither nasal lavage nor serum ECP levels were significantly correlated with symptom score, duration of disease, PRQLQ total score or domains (p > 0.05 for all). Conclusion: These results may indicate the absence of a correlation between clinical status and quality of life and levels of ECP in tissues with allergic inflammation. Copyright © 2008 S. Karger AG.Item Histologic and morphologic effects of valproic acid and oxcarbazepine on rat uterine and ovarian cells(2010) Cansu A.; Erdogan D.; Serdaroglu A.; Take G.; Coskun Z.K.; Gurgen S.G.Purpose: To determine the histologic and morphologic effects of valproic acid (VPA) and oxcarbazepine (OXC) on rat uterine and ovarian cells. Methods: Fifty-six female prepubertal Wistar rats (21-24 days old and weighing between 47.5 and 58.1 g) were divided equally into four groups, which were given drinking water (controls), 300 mg/kg/day of VPA, 100 mg/kg/day of OXC or VPA + OXC via gavage, for 90 days. Ovaries and uteri of rats on proestrous and diestrous phases of estrous cycle were extirpated and placed in a fixation solution. The tissue specimens were assessed with apoptosis (TUNEL) staining protocols, eosinophil counting, and electron microscopic techniques. Results: In uteri, apoptosis in stroma, mitochondrial swelling, and cristolysis were observed in the VPA group, and OXC led to negative effects on epithelial cell and intracellular edema. In ovaries, both drugs increased apoptosis and intracytoplasmic edema. Organelle structure disruption was also observed in the OXC group. More conspicuous degenerative modifications were determined in the VPA + OXC group. In uteri, the number of TUNEL-positive luminal epithelial cells was 7.20 ± 1.32 in controls, and significantly increased to 29.60 ± 1.58, 34.20 ± 2.53, and 54.80 ± 2.04 in VPA, OXC, and VPA + OXC groups, respectively (p < 0.001). The highest number of TUNEL-positive glandular epithelium cells was observed in the VPA + OXC group; however, the number of TUNEL-positive stroma cells was highest in the VPA group. The highest number of eosinophils in stroma was in the VPA group. Conclusion: VPA and OXC trigger apoptotic and degenerative effects on rat uterine and ovarian cells. VPA also prevents implantation of embryo to the uterus and causes abortion via endometrial eosinophil infiltration. © 2009 International League Against Epilepsy.Item E-cadherin as an epithelial barrier protein in exhaled breath condensate(Institute of Physics Publishing, 2014) Yuksel H.; Turkeli A.; Taneli F.; Horasan G.D.; Kanik E.T.; Kizilkaya M.; Gozukara C.; Yilmaz O.Airway epithelium plays an important role as a physical barrier and a modulator of allergic response. Junctions between cells provide epithelial integrity and barrier function. The aim of this study was to investigate the influence of atopy on airway epithelial integrity in asthma and to measure E-cadherin levels in exhaled breath condensate as an indicator epithelial damage. A total of 74 patients with asthma (35 atopic and 39 non-atopic) and 39 healthy children were enrolled in this case-control study. Sociodemographic characteristics and asthma severity parameters in the last three-month period were recorded and pulmonary function tests were performed. Blood samples were obtained to measure serum immunoglobulin E (IgE) levels and peripheral blood eosinophil count, and exhaled breath condensate (EBC) was obtained to measure E-cadherin. EBC E-cadherin levels were significantly lower in the asthmatics when compared to non-atopic controls (0.109 (0.076) versus 0.191 (0.184) ng mL-1 respectively, p = 0.01). Atopic and non-atopic asthmatic groups had lower EBC E-cadherin levels compared to the control group. (0.112 (0.060) ng ml-1, 0.106 (0.089) ng ml-1 and 0.191 (0.184) ng ml-1, p = 0.02 and p < 0.01 respectively). However, EBC E-cadherin levels were not different between atopic and non-atopic asthmatics. The results of our study support the role of E-cadherin in the pathogenesis of asthma. However, the absence of difference in E-cadherin levels between atopic and non-atopic asthmatics suggests that allergic sensitization is not the primary factor for development of epithelial barrier dysfunction in asthma. © 2014 IOP Publishing Ltd.Item Handling of allergen immunotherapy in the COVID-19 pandemic: An ARIA-EAACI statement(Blackwell Publishing Ltd, 2020) Pfaar O.; Klimek L.; Jutel M.; Akdis C.; Bousquet J.; Akdis M.; Bachert C.; Agache I.; Ansotegui I.; Bedbrook A.; Bosnic-Anticevich S.; Canonica G.W.; Chivato T.; Cruz A.A.; Czarlewski W.; Del Giacco S.; Du H.; Fonseca J.A.; Gao Y.; Haahtela T.; Hoffmann-Sommergruber K.; Ivancevich J.-C.; Khaltaev N.; Knol E.F.; Kuna P.; Larenas-Linnemann D.; Melen E.; Mullol J.; Naclerio R.; Ohta K.; Okamoto Y.; O’Mahony L.; Onorato G.L.; Papadopoulos N.G.; Pawankar R.; Samolinski B.; Schwarze J.; Toppila-Salmi S.; Shamji M.H.; Teresa Ventura M.; Valiulis A.; Yorgancioglu A.; Matricardi P.; Zuberbier T.[No abstract available]Item Incontinentia pigmenti Stage 1 is not simply vesiculo-bullous but vesiculo-pustular(John Wiley and Sons Inc, 2024) Çetinarslan T.; Fölster-Holst R.; Van Gysel D.; Buchner M.; Happle R.Incontinentia pigmenti (IP) is a rare X-linked dominant, male-lethal disorder characterized by pathognomic skin lesions. As described in the literature the typical cutaneous changes follow the pattern of Blaschko's lines and develop in four stages that usually start at birth. Stage 1 is called vesicular, bullous or inflammatory. The vesicles are rapidly filled with eosinophils and thus turn into pustules. Thus, the term “pustular” is relevant to the first phase of IP, and the stage can be considered as “vesiculopustular/inflammatory” to be more precise than “vesicular” or “bullous.”. © 2023 Wiley Periodicals LLC.