Browsing by Subject "PHYTATE"

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    Evaluation of Phytic Acid Content of Some Tea and Nut Products by Reverse-Phase High Performance Liquid Chromatography/Visible Detector
    (SPRINGER) Dost, K; Karaca, G
    Phytic acid contents of nine different types of nut and six different types of tea and steeped tea were analysed by reverse-phase high performance liquid chromatography with visible detector. The extraction method was based on hydrochloric acid extraction, and the analysis method was based on metal replacement reaction of phytic acid from coloured complex (iron(III)-thiocyanate), separation on CN column and monitoring the absorbance at 460 nm. The retention time for the monitored iron(III)-thiocyanate peak was achieved less than 3 min. The proposed HPLC/Vis procedure shows good linearity over the concentration range of 1-150 mg L-1 with a correlation coefficient value of 0.9938. The effectiveness of metal replacement reaction was presented in terms of relative standard deviation that was 0.62 and 0.88 % for 5 and 50 mg L-1 of phytic acid, respectively. Repeatability of the analytical method was ranging between 1.58-7.88 % (n = 10, for 50 mg L-1) and 0.98-4.63 % (n = 10, for 5 mg L-1) in terms of relative standard deviation. Accuracy of the method is good, ranging relative error between 4.52 and 8.00 % (n = 10, for 50 mg L-1). Phytic acid content is in the range of 1.54 to 9.74 mg g(-1) in nuts, 27.67 to 28.82 mg g(-1) in green teas, and 20.49 to 21.96 mg g(-1) in pocketed roasted teas.
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    Determination of phytic acid in wheat and wheat products by reverse phase high performance liquid chromatography
    (ELSEVIER) Dost, K; Tokul, O
    A sensitive method for accurate determination of phytic acid in food samples is described. The proposed procedure involves high performance liquid chromatography (HPLC) and UV-vis detection. The method was based on metal replacement reaction of phytic acid from coloured complex (iron(M)-thiocyanate), separation and monitoring any decrease in concentration of coloured complex. The retention time for the monitored iron(III)-thiocyanate peak was achieved in less then 3 min in Gaussian shape. The proposed HPLC/UV-vis procedure shows good linearity over the concentration range of 10-125 mu g ml(-1) with correlation coefficient value of 0.997. The procedure is reproducible and accurate, illustrating a standard deviation between 0.72 and 1.20 mu g ml(-1) (n = 10, for 50 mu g ml(-1)) and relative standard deviation between 1.40 and 2.98% (n = 10, for 50 mu g ml(-1)) within and between days for reproducibility and absolute error ranging between 0.16 and 1.54% (n = 10, for 100 mu g ml(-1)) within and between days for accuracy, respectively. Detection limit was estimated as 0.5 mu g ml(-1) which is suitable for analysis of foodstuffs and plant materials in that phytic acid usually presents within 2.5-50 mu g ml(-1) range. The procedure was then applied to determination of phytic acid in wheat and wheat products. The content of the phytic acid in the samples was calculated by using the calibration curve obtained from the standard solution of phytic acid and iron(IH)-thiocyanate solutions containing 100 mu g ml(-1) iron(RI) ion. (c) 2005 Published by Elsevier B.V.