Browsing by Subject "Phaeodactylum tricornutum"

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    Evaluation of scale-up methodologies and computational fluid dynamics simulation for fucoxanthin production in airlift photobioareactor
    (John Wiley and Sons Ltd, 2020) Aslanbay Guler B.; Deniz I.; Demirel Z.; Imamoglu E.
    Researches about commercial-scale production of fucoxanthin are increasing day by day due to the much interest of its broad beneficial health effects. The aim of this study was to investigate the scale-up methodologies in airlift photobioreactor and to simulate fluid flow using computational fluid dynamics (CFD) for fucoxanthin production from Phaeodactylum tricornutum microalgae. During the transition process from 1-L cultivation bottle to the 2-L airlift photobioreactor, the constant mixing time, the constant volumetric oxygen transfer coefficient, and the constant volumetric power consumption rate were evaluated as scale-up methodologies under laboratory conditions. Maximum fucoxanthin yield was found as 1.01 ± 0.07 mg g−1 using scale-up strategy based on constant volumetric power consumption rate. After that, CFD simulation was performed in order to examine flow characteristics, mixing efficiency, hydrodynamics, and gas holdup for selected operation conditions. It was observed that mixing efficiency was low because the draft tube configuration prevented the circulation of fluid and caused a heterogenic culture medium inside the reactor. It is possible to overcome this bottleneck by developing the effective draft tube. © 2020 Curtin University and John Wiley & Sons, Ltd.
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    A novel subcritical fucoxanthin extraction with a biorefinery approach
    (Elsevier B.V., 2020) Aslanbay Guler B.; Deniz I.; Demirel Z.; Yesil-Celiktas O.; Imamoglu E.
    Eco-friendly, cost efficient and effective extraction methods have become significant for the industries applying zero waste principles. The two main objectives of this study were; to examine fucoxanthin extraction from wet Phaeodactylum tricornutum using subcritical fluid extraction and to characterize the residual biomass in order to determine the potential application areas. The highest fucoxanthin yield of 0.69 ± 0.05 mg/g wet cell weight was achieved using methanol with solvent-to-solid ratio of 200:1 at 120 rpm, 20 MPa pressure and at 35 °C for 60 min by subcritical extraction. Microscopy images showed that most of the cells were disrupted and intracellular components were effectively released. Based on the results of energy dispersive spectroscopy, biomass contained a mixture of organic molecules including mainly carbon (57–72%), oxygen (26–41%), magnesium (0.6–1.4%) and silica (0.4–1%) (wt%). These results make the residual biomass a potential candidate for various areas such as bioenergy, material sciences and sensor technologies. © 2019 Elsevier B.V.