Browsing by Subject "Phytochemical composition"

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    Phenolic composition, antioxidant and enzyme inhibitory activities of acetone, methanol and water extracts of Clinopodium vulgare L. subsp. vulgare L.
    (Elsevier, 2015) Sarikurkcu C.; Ozer M.S.; Tepe B.; Dilek E.; Ceylan O.
    The aim of this study is to evaluate the antioxidant and enzyme inhibitory activities of acetone, methanol and water extracts of Clinopodium vulgare L. subsp. vulgare L. as well as its phytochemical composition. Methanol extract was found rich in of phenolic compounds (44.42. mg GAEs/g extract) whereas the highest flavonoid content was determined in the water extract (40.20. mg REs/g extract). Methanol extract was also found rich in protocatechuic acid, (+)-catechin, chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid and apigenin. Data obtained from the phosphomolybdenum assay was found in correlation with those obtained from total phenolic assay. Methanol extract exhibited the highest activity (374.86. mmol TEs/g extract). In the case of chelating effect assay, acetone extract exhibited the highest activity. As observed in many previously reported studies, water extract exhibited the highest DPPH free radical scavenging activity (81.72. mg TEs/g extract). ABTS cation scavenging assay was resulted in the superiority of methanol extract (51.45. mg TEs/g extract). It also showed the strongest reducing power in CUPRAC and FRAP assays. Acetone extract exhibited remarkable inhibitory activity on acetylcholinesterase, butyrylcholinesterase and tyrosinase, while the highest α-amylase and α-glucosidase inhibitory activities were exhibited by methanol and water extracts. Total amounts of unsaturated fatty acids were determined as 51.15%. C18:1 ω9, C18:2 ω6 and C18:3 ω3 were determined in considerable quantities (23.93, 6.18 and 5.78%, respectively). © 2015 Elsevier B.V.
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    Valorization of Pinus halepensis Mill. seed oil: Physicochemical characteristics, bioactive compounds, and antioxidant activity as affected by location and extraction method
    (Blackwell Publishing Ltd, 2021) Ait Atmane S.; Aksoylu Özbek Z.; Günç Ergönül P.; Khettal B.
    In this study, physicochemical characteristics, phytochemical compositions, and antioxidant activities of Pinus halepensis Mill. seed oils (PHSO) from two geographical origins (Algeria and Turkey) and obtained through two different extraction processes (cold pressing and solvent extraction) were determined. PHSO were majorly consisted of linoleic and oleic acids. Cold pressing yielded oils with lower peroxide and totox values, higher tocopherols contents (309.42–318.04 mg/100 g), higher DPPH-radical scavenging activities (25.33–26.94 mM TE/kg), and reducing capacity values (301.30–329.94 mg GAE/kg). The solvent-extracted oils contained more phytosterols (5,683.25–5,964.20 mg/kg), particularly β-sitosterol and campesterol. Phenolic compounds of PHSO were identified by HPLC-DAD for the first time in this study. More phenolic compounds were detected in solvent-extracted oils. Turkish PHSO were richer in individual phenolics (18,928.92 μg/100 g) than the Algerian variety (2,821.01 μg/100 g). Different extraction techniques and geographical origins led to differences in physicochemical and phytochemical attributes of PHSO. Novelty impact statement: This study revealed that extraction technique (cold-pressing, solvent extraction) and geographical seed origin (Algeria, Turkey) influenced the Pinus halepensis Mill. seed oils (PHSO) composition. PHSO are rich in fatty acids, sterols, and tocopherols majorly linoleic acid, γ-tocopherol, and β-sitosterol which were detected for first time for the cold pressed oils. This is the first report on phenolics compounds of these oils, mainly luteolin. The high oxidative stability and good antioxidant activities of PHSO promote their exploitation in food, cosmetics, and pharmaceuticals uses. © 2021 Wiley Periodicals LLC.