Browsing by Subject "REVASCULARIZATION"

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    Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium
    (PERGAMON-ELSEVIER SCIENCE LTD) Özdal-Kurt, F; Sen, BH; Tuglu, I; Vatansever, S; Türk, BT; Deliloglu-Gürhan, I
    Objective: We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). Methods: After isolation of DPSC, they were differentiated to ODPSC. Standard dentin discs from human molar teeth were prepared. While the dentin discs in Group 1 did not receive any extra treatment, the discs in Group 2 were treated with acidic calcium phosphate precipitation (CPP) solution. In Group 3, the discs were suspended in phosphate buffered saline containing calcium. DPSC or ODPSC (3 x 10(4) cells/mL) were seeded on all discs and incubated for 7,14 or 21 days. Attachment and growth of 7-day cell cultures on extra dentin samples were examined by SEM. MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Results: While DPSC and ODPSC showed similar proliferation rates at 7 and 14 days (p > 0.05), the number of ODPSC was higher than DPSC in 21-day samples (p = 0.039). MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p < 0.05). Calcium-treated dentin surfaces always had lower number of cells; being significant for only CPP-treated surfaces (p < 0.01). Both types of cells demonstrated good attachment and proliferation on dentin surfaces regardless of type of dentin treatment. Conclusions: Because the nature of dentin surface itself showed good adhesive characteristics with ODPSC and DPSC, additional calcium treatment of dentin surfaces may not be necessary. (C) 2016 Published by Elsevier Ltd.
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    A comparison of off-pump and cardiopulmonary bypass for the development of postoperative atrial fibrillation
    (BAYCINAR MEDICAL PUBL-BAYCINAR TIBBI YAYINCILIK) Besir, Y; Tetik, Ö; Lafçi, B; Gökalp, O; Günes, T; Gücü, A; Gürbüz, A
    Background: In this study, we aimed to investigate the possible effects of cardiopulmonary bypass and procedures for cardiac arrest in the etiology of atrial fibrillation (AF). Methods: Between January 2006 and January 2007, consecutive 449 patients who underwent elective coronary artery bypass graft (CABG) surgery in our clinic were retrospectively analyzed. The patients were divided in two groups. The first group included 86 patients (58 males, 28 females) who underwent off-pump coronary revascularization, whereas the second group included 363 patients (261 males, 102 females) who underwent coronary revascularization with cardiopulmonary bypass. The incidence of postoperative AF and some perioperative variables in both groups were studied and compared. Results: The incidence of postoperative AF was 21.4% in cardiopulmonary bypass group and 3.4% in off-pump group. Avoiding cardiopulmonary bypass resulted in a statistically significantly reduction in the incidence of postoperative AF. Conclusion: The incidence of AF following myocardial revascularization without cardiopulmonary bypass is considerably lower. Our study results suggest that off-pump procedure is very effective in reducing the incidence of postoperative AF.