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Item Total nasal reconstruction with free and local flaps(LIPPINCOTT WILLIAMS & WILKINS) Yoleri, L; Öztan, YItem Effects of alcohol during secondary neurulation in chick embryos(DR BEHCET UZ COCUK HASTALIKLARI VE CERRAHISI) Mete, M; Aydemir, I; Ünsal, ÜÜ; Özbilgin, K; Tuglu, MI; Gürcü, BObjective: Alcohol continues to be consumed even though its harmful effecs are well established. One of the most common damage of alcohol consumption is fetal alcohol syndrome, characterized by craniofacial anomalies, cardiac anomalies and neural tube defects. Therefore, understanding the molecular mechanisms underlying the alcohol-induced toxicity that occur with time and dose dependent manner is very important., Most of the studies in order to understand the effects of alcohol have been carried out on early neurulation, however its effects on late neurulation are still unknown. Therefore in this study, effects of alcohol on secondary neurulation were investigated in chick embryos. Methods: Leghorn breed of embryonic chicken eggs were used. At 50 h of incubation, 100 mu L 50% ethanol solution was injected. Depending on the period of exposure to alcohol, varying degrees of pathological disorders were detected in E3, E7 and E10 days. Results: Developmental delay, structural abnormalities, morphological abnormalities in the heart and face and especially presence of two spinal cord cavities were found. In addition, we also detected delays in the closure of the neural tube, cellular deformities and the structural abnormalities in notochord. While eNOS, iNOS, and TUNEL levels increased, while laminin levels decreased. Conclusion: In this study during late development, significant alcohol-induced morphological and histopathological changes were observed. We also determined Increased level of oxidative stress caused by alcohol was accompanied with the changes in matrix composition. Better understanding of these mechanisms which affect the cell behavior is important and will allow learning of harmful effects of alcohol.Item Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system(ELSEVIER GMBH, URBAN & FISCHER VERLAG) Deliloglu-Gurhan, SI; Vatansever, HS; Ozdal-Kurt, F; Tuglu, IBone marrow is a complex tissue composed of hematopoietic and stromal. stem cells with the potential to differentiate into adipogenic, fibroblastic, reticular, osteogenic and chondrogenic lineages. Identification of differentiation markers during transformation of stromal cells into osteoblasts in a time-dependent manner may be informative for cell-based tissue engineering. Therefore, we investigated the effects of osteogenic medium (OM) on the proliferation and differentiation of rat bone marrow stromal. cells (BMSCs). BMSCs from adult mate rat tibia and femur were collected and cultured in alpha-MEM medium with 10% fetal bovine serum, penicillin, streptomycin and gentamycin. After three days of culture, the medium covering the adherent cells in culture was changed to OM containing dexamethasone, Na-beta-glycerophosphate and ascorbic acid. As a control., cell. culture was also continued in the original. medium for the same time period. Differentiated osteoblast cells were collected after 7, 10, 14, 21 and 30 days of culture, fixed with 4% paraformaldehyde and their immunolabelling for osteoblast markers osteonectin (ON) and osteocalcin (OC) was assessed using an indirect immunoperoxidase technique. Immunoabelling of ON and OC was detectable from day 10 of culture, began to increase on day 14, and increased steadily through to day 21. Labelling was highest on day 30 and was more intense in cells cultured with OM compared to the culture without OM. The control cells cultured in the absence of OM produced negligible levels of both markers. In conclusion, our culture system facilitated differentiation of BMSCs into osteoblasts featuring osteoblast markers, and these cells may be useful in autologous bone implant for the treatment of bone wound heating. (C) 2005 Elsevier GmbH. All rights reserved.Item A New Staining Method for Hand-Cut in Plant Anatomy Studies(MARMARA UNIV, FAC PHARMACY) Bozdag, B; Kocabas, O; Akyol, Y; Özdemir, CIn this study, a new double staining method that provide clearly to identify tissues in making preparations by hand-cut of Plant samples were fixed with alcohol has been developed. This new method can be provides practicality and certainty in terms of distinguishing the tissues and can be used for hand-cut. In this' method, double staining consist of safranin and fast green can be used in both monocotyledons and dicotyledons and can be stored at room temperature for a long time without spoiling. Photographs of the anatomical sections is used this method was taken and tissues was demonstrated.Item Adipose-Derived Mesenchymal Stem Cell Transplantation in Chemotherapy-Induced Premature Ovarian Insufficiency: the Role of Connexin and Pannexin(SPRINGER HEIDELBERG) Sen Halicioglu, B; Saadat, KASM; Tuglu, MIIn women undergoing chemotherapy, it is inevitable that infertility risk will increase because of impaired reproductive functions. Premature ovarian insufficiency (POI), which occurs as a devastating result of chemotherapy, is the complete depletion or dysfunction of ovarian follicles. Adipose-derived mesenchymal stem cells (ADMSCs) transplantation is among the alternative treatment methods for POI, which currently do not have an effective treatment method. Apoptosis of granulosa cells in POI is seen as the main mechanism of the disease. It is also reported that in addition to molecules directly associated with apoptosis, connexins, and pannexins are also potential effector molecules in apoptosis. The roles of these molecules in POI, which are known to play a role in many important mechanisms in the ovary, are unknown. In this study, it was aimed to analyze the expressions of Connexin43 and Pannexin1, which are thought to be effective in the formation of POI, and to show the relationship between the antiapoptotic effects of ADMSCs transplantation and these molecules in POI. For this purpose, Caspase3, Connexin43, Pannexin1 proteins, and mRNA expressions were analyzed by immunohistochemistry and RT-qPCR, and AMH levels were measured by ELISA. It was determined that Pannexin1, Caspase3 proteins, and mRNA levels increased in the POI, while Pannexin1 and Caspase3 expressions decreased in the ADMSCs treated group. While Connexin43 level decreased in POI, Connexin43 protein and mRNA levels increased in ADMSCs group. Consequently, this study demonstrated for the first time that Connexin43 and Pannexin1 were associated with apoptosis in POI. In addition, it was revealed that ADMSCs transplantation could produce antiapoptotic effects by modulating these molecules.Item The Relationship of Testicular Sonoelastography with Gonadotropin Hormone Levels and Sperm Parameters(WOLTERS KLUWER MEDKNOW PUBLICATIONS) Bozkurt, YE; Gümüs, BH; Özbay, M; Düzgün, F; Taneli, F; Kurutep, SBackground: Elastography is a non-invasive medical imaging technique that helps determine the stiffness of organs and other structures in our body. In this study, we investigated the effectiveness of elastography in the diagnosis of infertility. Aim: In this study, we aimed to examine the relationship between testicular elastography and hormonal parameters and sperm parameters. Patients and Methods: The study included 136 patients, 272 testicles were examined, and the mean age of the study participants was 30.1 years. Testicular tissue stiffness was measured by scrotal ultrasonographic shear wave elastography. Gonadotropin and testosterone hormones were measured from blood samples. Spermiogram parameters were studied manually. Results: The control group included 66 patients, and the varicocele group consisted of 70 patients. Testicular stiffness degrees of the control group were measured as 4.29 kPa for the right testis and 4.23 kPa for the left testis. The varicocele group was divided into grades 1, 2, and 3 according to physical examination. In group 1 (grade 1), the right testis was 4.07 +/- 1.24 kPa and the left testis was 3.77 +/- 0.98 kPa. In group 2 (grade 2), the right testis was 4.31 +/- 1.40 kPa and the left testis was 3.98 +/- 0.93 kPa. In group 3 (grade 3), the right testis was 4.73 +/- 1.50 kPa and the left testis was 3.99 +/- 1.68 kPa. Hormone and sperm parameters were not statistically significant when comparing the control and varicocele groups. There was no statistical significance between the testicular tissue stiffness degrees of the control and varicocele groups. Hormone and spermiogram findings were also similar in groups. Conclusion: It is known that varicocele leads to histological tissue changes in the testes. These changes result in tissue softness and loss while affecting sperm parameters and testosterone levels in a negative way. Before varicocele surgery, there is a need for new imaging methods with more sensitivity that can detect tissue changes in the testes.Item Cardiac functions in children with growth hormone deficiency: Effects of one year of GH replacement therapy(CHURCHILL LIVINGSTONE) Alkan, F; Ersoy, B; Kizilay, DO; Coskun, SIntroduction: Children with Growth Hormone deficiency (GHD) are prone to heart dysfunction and, if left untreated, will result in marked cardiac dysfunction in adulthood. The aim was to evaluate the effect of GHD and growth hormone (GH) therapy on cardiac structure in children and adolescents, and to investigate the role of insulin like growth factor-1 (IGF-1) in this. Methods: M-mode, pulse-wave Doppler echocardiography and tissue Doppler imaging (TDI) were performed in 49 children with GHD who were divided into those with a peak GH response < 7 mu g/L and 7-10 mu g/L after two GH stimulation tests, aged 8-16 years at baseline and at six and 12 months after GH initiation, and 49 healthy peers. IGF-1 concentration was measured. Results: Although the left ventricular end diastolic and systolic diameters in both GH deficient groups were significantly lower than controls (p < 0.01), both diameters increased significantly with one year of treatment and achieved normal values (p > 0.05). Using TDI in both two patients group revealed increased E/A, prolonged isovolumic relaxation time, shortened ejection time, and a significant increase in myocardial performance index compared to controls (p < 0.001). Significant improvement was observed in these parameters from the sixth month of GH treatment (p < 0.001), this improvement does not match parameters measured in healthy peers, even after one year of treatment in both patients group. (p < 0.001). No correlation was found between IGF-1 concentration and any echocardiographic parameter. Conclusion: Echocardiographic parameters were similar in children with a GH peak < 7 mu g/L and 7-10 mu g/L. In TDI, both systolic and diastolic function was impaired in GHD children compared to controls. These parameters improved after one year of GH therapy but did not recover to healthy control levels.Item Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma(ELSEVIER GMBH) Sanci, M; Dikis, C; Inan, S; Turkoz, E; Dicle, N; Ispahi, CAngiogenic factors, such as vascular endothelial growth factor (VEGF), its receptors and epidermal growth factor receptor (EGF-R), are involved in increased progression in many carcinomas. The aim of this study was to investigate the role of angiogenesis and immunolocalization of VEGF, its receptors, EGF-R and Ki 67 in leiomyomas and leiomyosarcomas using an indirect immunohistochemical method. Samples from patients with leiomyoma, cellular leiomyoma and cellular leiomyosarcoma (n=20 per group) were fixed in 10% formalin and processed using routine paraffin protocols. Following initial histological analysis, samples were immunostained with primary antibodies for VEGF, VEGFR-1, VEGFR-2, EGF-R and Ki-67 using an indirect avidin-biotin peroxidase method. Immunostaining intensities were evaluated as mild, moderate or strong and a semi-quantitative method (H-Score) was used to compare the samples. While mild/moderate EGF-R immunostaining and moderate immunostaining for VEGF and its receptors were observed in samples of leiomyomas, much less immunoreactivity was observed in cellular leiomyomas. All immunoreactivities and immune-stained cells increased in leiomyosarcomas. When scores of intensity and percentage of positive staining cells were compared, all immunoreactivities were shown to be significantly increased in leiomyosarcomas compared to leiomyomas. These results suggest that in leiomyosarcoma, angiogenic factors, such as VEGF, its receptors and EGF-R, may be involved in tumor angiogenesis. Active tumor cells can trigger angiogenesis, interaction with surrounding tissue and in the tissue itself initiating angiogenic activity. Angiogenic growth factors play an important role and induce malignant transformation through both autocrine and paracrine mechanisms. Anti-angiogenic agents may provide a novel therapeutic approach for the treatment of leiomyosarcoma. (C) 2010 Elsevier GmbH. All rights reserved.Item Biochemical analysis of urethral collagen content after tubularized incised plate urethroplasty: an experimental study in rabbits(SPRINGER) Taneli, F; Ulman, C; Genc, A; Yilmaz, O; Taneli, CThe aim of the present study was the biochemical analysis of tissue hydroxyproline levels in incised urethral plates in order to show the total collagen content after the Snodgrass operation in the hypospadiac rabbit model. The study comprised 21 male New Zealand rabbits, (2.2-2.4 kg). The animals were randomly allocated to three groups each containing seven rabbits as follows: group 1, the ventral urethra was completely excised and a model of hypospadias formed. A full-thickness incision was made on the distal dorsal urethra, a feeding tube was placed as an urethral catheter and both urethral wings were sutured ventrally. Group 2, inserting an iris knife into the urethra, the ventral wall was incised mimicking an urethrotome. Group 3 consisted of normal control rabbits to determine the basal tissue hydroxyproline level. A slight increase in the hydroxyproline level was observed in the ventral part of the urethral tissue compared to the dorsal part in both groups 1 and 2; however, these differences were not significant. After the Snodgrass operation in the rabbit model, no significant differences were observed in the hydroxyproline levels of the dorsal and ventral parts of the urethra or between these and of the controls. Further studies are required in order to determine the mechanism underlying urethral healing through normal re-epithelization without excess collagen deposition after incised urethral plate urethroplasty.Item The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts(TAYLOR & FRANCIS LTD) Özdal-Kurt, F; Tuglu, I; Vatansever, HS; Tong, S; Sen, BH; Deliloglu-Gürhan, SIWe investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.Item Finite element method simulation for the prediction of mechanical properties of three-dimensional periodic bioactive glass scaffolds(SPRINGER) Deliormanli, AM; Deliormanli, AHThe desired mechanical properties of porous tissue engineering scaffolds may differ depending on the clinical applications. Therefore, it is crucial to be able to control these properties for specific cases. In the current study, cube shape, porous, silicate-based (13-93) bioactive glass scaffolds were fabricated by robotic deposition method. Scaffolds were prepared layer by layer to form constructs with a grid-like microstructure. After binder burnout, the constructs were sintered for 1 h at 700 degrees C to produce scaffolds consisting of dense bioactive glass struts (similar to 280 +/- 20 aem in diameter) at different pore widths (300 +/- 50, 600 +/- 25, and 900 +/- 50 aem). The mechanical response of the scaffolds in compression was measured experimentally. The stress analysis of the complete scaffolds with varying pore width and layer spacing parameters has been performed by finite element method (FEM) under compression to investigate the state of stress fields created within the scaffolds. Such an analysis can be used to vary several geometrical parameters and to choose the most suitable ones for the replacement of natural tissues. The compressive strengths predicted by the FEM simulations were successfully validated by comparison with experimental uniaxial compression test data, justifying the suitability of the present approach for the optimization purposes.Item In Vitro Cultivation, Characterization and Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth on 3D Printed Polylactic Acid Scaffolds(KOWSAR PUBL) Islam, A; Mammadov, E; Kendirci, R; Aytac, E; Cetiner, S; Vatansever, HSBackground: Tissue engineering mainly focuses on creating appropriate conditions for the regeneration of tissues. Scaffolds, signal molecules, and stem cells interact with each other and compose the essential components of this field. Objectives: This study aimed at investigating the osteogenic induction ability of PLA Poly Lactic Acid (PLA) scaffolds and comparing the osteogenic differentiation behavior of Stem Cells from Human Exfoliated Deciduous Teeth (hSHEDs) in standard culture medium and on PLA scaffolds. Methods: The current clinical experimental study was conducted between April 2016 and October 2016 at the Near East University cell culture laboratory located in North Cyprus. The pulp tissues of deciduous teeth (non-decayed and in the absence of abscess, fistula or periapical lesion) were sampled from 10 healthy children aged between 6 and 11 years. The isolated hSHEDs were divided to 4 groups. The control group/Group1 consisted of cells, which were cultivated in standard culture medium, and Group2 cells were differentiated into an osteogenic lineage using osteogenic differentiation medium. Group 3 represented the non-differentiated group, which was transferred onto three dimensional (3D) printed PLA scaffolds and Group 4 cells were differentiated to the osteogenic lineage and transferred onto 3D printed PLA scaffolds. All groups were analyzed immunohistochemically and by immune-labeling, and were evaluated semi-quantitatively using the HSCORE. Results: Cultivation of hSHEDS on PLA scaffolds was assessed for 14 and 21 days; osteogenic differentiation was detected both histochemically and immunohistochemically. Generally, Osteocalcin (OCN) immunoreactivities were higher than Osteonectin (ON) immunoreactions in all groups. Despite higher OCN immunoreactivities, the intensities of OCN between 14 days and 21 days in group 4 (497.3 +/- 0.57% and 486.7 +/- 5.77%, respectively) were similar (P > 0.05). While the intensity of ON was 280.0 +/- 10% in group 4, in group 2 the intensity of ON was 206.7 +/- 5.77%, and on the 14th day the results were statistically significant (P < 0.0001). Conclusions: Poly lactic acid is a suitable scaffold material for osteogenic induction of the hSHEDs. The expression patterns of both markers showed that a 14-day cultivation period is adequate for hSHEDs with/without PLA scaffolds to differentiate into osteoblasts.Item The effect of tadalafil on anastomotic healing in ischemic small intestine in rats(SPRINGER) Kaya, Y; Coskun, T; Ayhan, S; Kara, E; Sakarya, A; Var, ATo investigate the effect of tadalafil on anastomotic healing in an ischemic small intestine. Standardized transection and anastomosis in the small intestine were performed in 48 male Sprague-Dawley rats divided into four equal groups (n = 12): group 1, normal anastomosis; group 2, ischemic anastomosis; group 3, normal anastomosis+tadalafil treatment; group 4, ischemic anastomosis+tadalafil treatment. Ischemia was established by ligating 2 cm of mesentery on either side of the anastomosis. Tadalafil was given to the rats once a day at dose of 5 mg/kg. The anastomotic bursting pressures and hydroxyproline concentrations were measured on postoperative day 4. A histopathological evaluation of the anastomoses was also performed. The bursting pressure and hydroxyproline concentration in group 2 were significantly lower than those in the other groups. There was no difference in the hydroxyproline concentration among groups 1, 3, and 4. While there was no difference between groups 3 and 4, the bursting pressures were significantly higher in groups 3 and 4 than in group 1. The histopathological evaluation revealed no significant differences in inflammatory cell infiltration, vascularization, or anastomotic collagen deposition among the groups. Tadalafil treatment improved the anastomotic bursting pressure and the hydroxyproline concentration in both normal and ischemic small intestine anastomosis.Item DETERMINATION OF SWEETPOTATO [Ipomoea batatas (L.) Lam.] GENOTYPES SUITABLE TO THE AEGEAN REGION OF TURKEY(SOC FIELD CROP SCI) Yildirim, Z; Tokusoglu, Ö; Öztürk, GA total of ten sweetpotato genotypes were grown in a field trial in 2003 and 2004 and certain agronomical and quality characteristics were determined. Local variety Hatay Kirmizi (Hatay Red) was selected as suitable to the Aegean Region for storage root number (7.7) and storage root yield (8.1 tons /ha) as well as acceptable quality characteristics: dry matter content: 41.8 %; sugar content: 2229.3 mg /kg; beta eta carotene 7.03 mg/100 g; vitamin A: 11716.3 IU; vitamin C: 38.6 Mg /100 g. Another introduced variety Regal was also selected for yield (6.6 ton /ha) and starch content (31.1 g/100 g), Beta carotene (7.04 mg /100 g) and vitamin A (11745 IU) for the Aegean Region.Item Histological and Biochemical Investigation of the Effects of Low Intensity Pulsed Ultrasound on Orthodontic Tooth Movement(DUZCE UNIV, FAC MEDICINE) Cesur, MG; Onal, T; Bilgin, MD; Sirin, FB; Inan, S; Koken, EC; Alkan, A; Cesur, GObjective: The goal of our study is to assess the effects of low intensity pulsed ultrasound on orthodontic tooth movement in rats. Methods: For this study, 40 adult male Wistar albino rats (12-weeks old age) were used from the Animal Laboratory at Adnan Menderes University. Rats were divided into four groups each of ten. Group 1 was the untreated as a control. In group 2, an orthodontic spring was used to move teeth. For groups 3 and 4, orthodontic treatment was combined with low intensity pulsed ultrasound at 16 J/cm2 or 48 J/cm2 for 14 days, respectively. Tooth movement was measured at the last day of treatment. Serum bone alkaline phosphatase (BALP) and C-telopeptide type I collagen (CTX-I) levels were analyzed biochemically. The number of osteoclasts, osteoblasts and inflammatory cells, capillary density and new bone formation was determined histologically. Receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta) were assessed using immunohistochemical staining. Results: BALP and CTX-I levels in group 4 were significantly higher compared to control (p<0.05). Tooth movement and the number of osteoclasts, inflammatory cells and capillary density in group 4 were significantly greater than group 2 (p<0.05). The intensity levels of RANKL and OPG in group 4 were significantly greater than group 2 (p<0.05). Conclusions: Ultrasound is a noninvasive application and promising therapy for accelerating bone remodelling during orthodontic tooth movement.Item Effect of pore architecture on the mesenchymal stem cell responses to graphene/polycaprolactone scaffolds prepared by solvent casting and robocasting(SPRINGER) Deliormanli, AM; Atmaca, HIn the study graphene-containing porous, three dimensional polycaprolactone (PCL) scaffolds were prepared by solvent casting-salt leaching and robocasting methods for tissue engineering applications. Graphene nanopowders in the form of nanoflakes were incorporated into the polymer matrix at different concentrations namely 1, 3, 5 and 10 wt%. The dichloromethane was used as the solvent and sodium chloride crystals were utilized as the water-soluble porogen for the formation of an interconnected porous network (with non-oriented pores) inside the composite scaffolds in solvent casting-salt leaching method. On the other hand, acetone was utilized as solvent and PCL solutions were prepared at 20 wt% in robocasting method to construct scaffolds (with oriented pores) having a grid-like structure. The biological response of bone marrow mesenchymal stem cells seeded on these composite constructs having different architecture were tested using MTT method, live-dead cell viability assay and Alcian blue stanining. Cytotoxicity experiments revealed that mesenchymal stem cells did not show toxic response to composite robocast scaffolds. Cells proliferate and differentiate well on the surface of the robocast scaffolds compared to solvent-cast scaffolds under the same conditions. Results showed that scaffolds prepared in the study have potential to be used in cartilage tissue engineering in the presence of electric stimulation.Item The significance of venous dominance in color Doppler ultrasound for the diagnosis of primary nodular skin lesions(ELSEVIER SCIENCE INC) Özkol, M; Yoleri, L; Demir, MA; Dernireli, P; Pabusçu, YThe aim of this study was to investigate the significance of color Doppler ultrasound (CDUS) findings in the differential diagnosis of suspicious nodular skin lesions and to compare the results according to the Giovagnorio 1999 classification and the modified classification. Forty nodular skin lesions were evaluated with CDUS and US. The number of arteries and veins was recorded in hypervascular lesions. Findings were compared with histopathological results. The specificity and predictivity of the modified classification were higher than those of the Giovagnorio 1999 classification. (c) 2006 Elsevier Inc. All rights reserved.Item Adiponectin Levels in Gestational Diabetes Mellitus and in Pregnant Women Without Glucose Intolerance(WROCLAW MEDICAL UNIV) Pala, HG; Ozalp, Y; Yener, AS; Gerceklioglu, G; Uysal, S; Onvural, AObjectives. The aim of the study was to determine serum adiponectin levels among patients with gestational diabetes mellitus (GDM) and normal pregnant women without glucose intolerance, and to investigate the relationship between these levels and clinical factors at the time of the diagnosis, at delivery and in the post-partum period. Material and Methods. The subjects' serum adiponectin concentration was measured using the enzyme-linked immunosorbent assay (ELISA) method at 24th-28th week of gestation, at delivery (in maternal circulation and the umbilical cord) and 24 h after delivery. The relationship between these groups' measurements and other established clinical-laboratory factors were investigated. Results. Serum adiponectin concentrations were significantly lower (p = 0.02) in GDM patients compared with patients with normal glucose tolerance at 24th-28th week of gestation. During delivery, maternal serum adiponectin concentrations were significantly lower (p = 0.03) in GDM patients compared with patients with normal glucose tolerance. In the post-partum period, serum adiponectin concentrations were significantly higher (p = 0.009) in GDM patients compared with patients with normal glucose tolerance. Umbilical cord adiponectin concentrations were significantly lower (p = 0.005) in GDM patients compared with patients with normal glucose tolerance. Conclusions. Adiponectin concentrations in GDM patients' circulation were regulated by changes in glucose and insulin metabolism. A reduction in serum adiponectin levels seems to play a role in GDM patients' insulin resistanceItem A Giant Paratesticular Liposarcoma(GALENOS YAYINCILIK) Erbatu, O; Nese, N; Müezzinoglu, TLiposarcoma is a tumour originating from the mesoderm, which captures nearly twenty percent of all sarcomas. It first described by Lesauvage in 1845. Seventy percent of cases are extremity and retroperitoneum masses. Paratesticular liposarcoma is a rare entity. For treatment, a tumour-free margin radical orchiectomy with wide local excision and high ligation of the spermatic cord should be performed. If needed radiotherapy should be applied. An eighty-six-year-old male patient with paratesticular liposarcoma is reported in this article.Item Prechondrogenic ATDC5 cell response to graphene/multi-walled carbon nanotube-containing porous polycaprolactone biocomposite scaffolds(TAYLOR & FRANCIS AS) Deliormanli, AM; Atmaca, HIn the study graphene and multi- walled carbon nanotube (MWCNT) - containing polycaprolactone (PCL) scaffolds were prepared by solvent casting-particulate leaching method for cartilage tissue engineering applications. Graphene nanopowders or MWCNTs were added to the system at different concentrations namely 1, 3, 5 and 10 wt%. Mechanical, thermal and electrical properties of the scaffolds were measured as a function of additive concentration. Response of prechondrogenic ATDC5 cells seeded on the composite constructs were tested using XTT method and live-dead cell viability assay. Alkaline phosphatase activity of the cells was also tested to investigate differentiation behaviour. Results showed that electrically conductive composite scaffolds prepared in the study have potential to be used in tissue engineering.