Browsing by Subject "Tissue"
Now showing 1 - 20 of 36
Results Per Page
Sort Options
Item Histopathological effects of sildenafil citrate on rat corpus cavernosum(Elsevier GmbH, 2004) Gümüs B.; Vatansever H.S.; Müezzinoǧlu T.; Müftüoǧluc S.; Kaymaz F.; Büyüksu C.Sildenafil citrate (Viagra) is widely used for the treatment of erectile dysfunction with various etiologies. The aim of the present study was the investigation of histopathological effects of sildenafil citrate on rat corpus cavernosum using light and electron microscopical techniques. Twenty male rats were divided into two groups. The first group (n = 10) was used as a control and the second group (n = 10) was treated with sildenafil citrate. Penile tissue was collected, fixed with formalin and embedded in paraffin for light microscopy, or fixed with gluteraldehyde and osmium tetroxide and embedded in Epon for electron microscopy. Light microscopical analysis showed that the corpus cavernosum was elongated and the number of blood vessels was increased. The amount of connective tissue in the penis was increased and dense collagen and smooth muscle fibers were observed in treated rats. Electron microscopical analysis showed that stromal structures of the corpus cavernosum (collagen fibers and number of cellular elements) were increased in treated rats. Fibroblasts showed signs of activation and the number of other stromaL cells was increased. Immature newly synthesized collagen fibers were observed and penetrated endothelial basement membranes. In addition, endothelial cells also showed signs of activation such as cytoplasmic granules in treated rats, whereas the surface area of blood vessels was increased and basement membranes were thickened. These histopathological changes due to treatment with sildenafil citrate indicate that prolonged use of sildenafil citrate may increase the risk of fibrosis in the penis. © 2003 Elsevier GmbH. All rights reserved.Item Beef longissimus lumborum, biceps femoris, and deep pectoralis Warner-Bratzler shear force is affected differently by endpoint temperature, cooking method, and USDA quality grade(2004) Obuz E.; Dikeman M.E.; Grobbel J.P.; Stephens J.W.; Loughin T.M.Effects of endpoint temperature, cooking method, and quality grade on Warner-Bratzler shear force (WBSF) of beef longissimus lumborum (LL), biceps femoris (BF), and deep pectoralis (DP) muscles were evaluated. Eighteen of all three subprimals were selected from USDA Select and 18 from USDA Choice (Certified Angus Beef) carcasses for the respective muscles. Muscles were vacuum packaged and held at 1 °C for 14 days, frozen (-29 °C), sawed into 2.54-cm thick steaks, vacuum packaged, and stored frozen until cooking. Thawed steaks were cooked by either a Magikitch'n® electric belt-grill (BG) at 93 °C, or a water-bath at 93 °C, to one of nine endpoint temperatures: 40, 45, 50, 55, 60, 65, 70, 75, or 80 °C. Belt-grill cooking was much faster and resulted in distinctly less cooking loss than water-bath cooking. Water-bath cooking resulted in higher (P < 0.0001) Instron ® WBSF (31.92 N) than BG (28.25 N) for LL. The combination of Select quality grade and higher endpoint temperatures resulted in higher (P < 0.05) WBSF for LL. Two distinct phases of tenderization/toughening occurred for BF. Between 40 and 60 °C, WBSF decreased from 43.95 to 38.16 N (P < 0.01), whereas between 60 and 70 °C, WBSF increased from 38.16 N to 44.44 N (P < 0.05). Water-bath cooling resulted in higher (P = 0.0001) DP WBSF (71.12 N) than BG (59.25 N). The DP had a distinct (P < 0.0001) decline in WBSF between 45 and 65 °C, irrespective of the cooking method, followed by an increase between 65 and 80 °C (P < 0.01). © 2004 Elsevier Ltd. All rights reserved.Item Computer assisted image analysis of peroxidase stained endometrial tissue(2006) Toprak A.B.; Vatansever S.; Özbilgin K.Aim: In immunohistochemistry, antibodies are used to detect and localize antigens in cellular compartments in sections taken from frozen or paraffin blocks. Currently, computerized image processing techniques are widely available to analyze immunohistochemically stained tissues; however, the image analysis programs are generally highly specific and expensive. We aimed to show the feasibility of computer assisted image analysis (CAIA) by using two computer programs currently in widespread use. Methods: Two available computer programs, Adobe Photoshop CS and ImageJ, were used to separate areas in the same tissue sections stained with NOS antibody and labeled with peroxidase in human endometrial tissue. CAIA was made on the relevant images using these two programs. Results: There was a significant difference among the groups according to staining intensity, in all three grades of staining (P < 0.001, P < 0.05, and P < 0.001, respectively). Subjective assessment of the images was in good agreement with objective CAIA. One (+) positive stained area was greater in group 1 and less in group 2 and group 3 (P < 0.001 and P < 0.001, respectively). Group 3, which was considered as the most intensely stained by the observer, had the greatest amount of ++ staining. Conclusion: CAIA is a safe and reliable method of analyzing tissue samples stained using immunohistochemical methods. © TÜBİTAK.Item Hepatic perfusion changes in an experimental model of acute pancreatitis: Evaluation by perfusion CT(Elsevier Ireland Ltd, 2010) Tutcu S.; Serter S.; Kaya Y.; Kara E.; Neşe N.; Pekindi G.; Coşkun T.Purpose: It is known that acute pancreatitis may cause secondary changes in several organs. Liver is one of these involved organs. In different experimental studies hepatic damages were shown histopathologically in acute pancreatitis but there are a few studies about perfusion disorders that accompany these histopathologic changes. Perfusion CT (pCT) provides the ability to detect regional and global alterations in organ blood flow. The purpose of the study was to describe hepatic perfusion changes in experimental acute pancreatitis model with pCT. Materials and methods: Forty Sprague-Dawley rats of both genders with average weights of 250 g were used. Rats were randomized into two groups. Twenty rats were in control group and 20 in acute pancreatitis group. pCT was performed. Perfusion maps were formed by processing the obtained images with perfusion CT software. Blood flow (BF) and blood volume (BV) values were obtained from these maps. All pancreatic and liver tissues were taken off with laparotomy and histopathologic investigation was performed. Student's t test was used for statistical analyses. Results: In pCT we found statistically significant increase in blood volume in both lobes of liver and in blood flow in right lobe of the liver (p < 0.01). Although blood flow in left lobe of the liver increased, it did not reach statistical significance. Conclusion: The quantitative analysis of liver parenchyma with pCT showed that acute pancreatitis causes a significant perfusion changes in the hepatic tissue. Systemic mediators seem to be effective as well as local inflammatory changes in perfusion changes. © 2009 Elsevier Ireland Ltd.Item The seasonal distribution of heavy metals in Mussel sample from Yalova in the Marmara Sea, 2008-2009(2011) Türk Çulha S.; Koçbaş F.; Gündocdu A.; Baki B.; Çulha M.; Topçuoclu S.Concentration of the some heavy metals (Co, Cu, Fe, Ni, Pb and Zn) Mediterranean mussel Mytilus galloprovincialis from Yalova (Marmara Sea) were investigated seasonally. The mean concentrations of Co, Cu, Fe, Ni, Pb and Zn in the soft tissues were 4.08 ± 0.67, 5.54 ± 0.59, 156.72 ± 20.18, 3.71 ± 1.14, 2.92 ± 0.51 and 106.23 ± 5.66 mg kg -1 dry weights, respectively. There were no significant differences between the metal concentrations among the seasons (P> 0.05). The mean Pb and Zn concentrations were higher than the acceptable limit proposed by International Turkish standards. © 2011 Springer Science+Business Media B.V.Item Effect of slice step size on prediction of natural vibration properties of bone tissue(Association for Scientific Research, 2012) Altintas G.Several vibration analysis procedures are used for determination of the level of bone loss, status of implant stability, modal damping factor and numerous other properties of tissues. The detection methods of bone properties are to compare the results of theoretical work with practical results. So, there are many options for processing of image data and establishing the finite element (FE) model that differentiation of calculated outputs is inevitable. Uncertainty of outputs can lead to mistakes while mechanical parameters or behaviors of tissue are determined. In this study, the effect of Micro-CT scanning intensity in connection with the reconstruction process on properties of the modal behavior of bone tissue were investigated. Results have shown that examined parameters have important effects on numerical values of the natural frequencies and modal behaviors. Furthermore, it has been revealed that numerical values and mode shapes must be considered together for properly understanding the natural vibration analysis of bone tissue.Item Effect of 3D isotropic resolutions of sequenced images on natural vibration properties of trabecular bone(Sharif University of Technology, 2013) Altintas G.; Ergut A.; Goktepe A.B.The voxel based finite element (FE) method used to obtain primary data for non-invasive imaging techniques has emerged as a major focus of interest in several disciplines such as medicine, mechanics and material engineering for solving micro and nano-scale problems. Owing to the fact that, voxel based FE models are directly affected by parameters of imaging techniques such as Computed Tomography (CT) and Magnetic Resonance Imaging (MRI), the consequences of these effects on the natural vibration analysis of structures having complex geometry in micro scale, are investigated in this study. In this context, voxel based FE models are obtained using Micro-CT imaging data that has three different resolutions of vertebral trabecular bone tissue. Furthermore, resolutions of image data sets are artificially increased and equalized for evaluating voxel based FE models that are free from FE size effects. Natural vibration characteristics of voxel based FE models are investigated not only numerically but also including associated mode shapes. Unpredictable vibrational behavior for various voxel sizes, is, thus, revealed. Element size effects of voxel based FE models are considerably different from the effects on structural components with regular prismatic shapes. Obtained results show that, investigated parameters have a crucial influence on the natural vibration behavior of trabecular bone tissue which is selected as an example of complex geometries. Modal behaviors that are effective in micro local regions, but less in the whole body, where there are possibilities for working with approximate geometry without considering the micro structure have been observed. Moreover, the results are new from a theoretical point of view, and they also represent the importance of quality in imaging data, which, in practical applications must be taken into consideration. © 2013 Sharif University of Technology. Production and hosting by Elsevier B.V. All rights reserved.Item In vitro assessment of degradation and mineralisation of V2O5substituted borate bioactive glass scaffolds(Maney Publishing, 2014) Deliormanli A.M.Bioactive glasses are promising scaffold materials for bone regeneration because of their unique ability to convert to hydroxyapatite (HA) in vivo, and their ability to bond with bone and soft tissues. In this study, borate based (13-93B3) bioactive glass powders containing up to 3 wt- %V2O5were prepared by the melt quench technique. Vanadium was chosen because of its therapeutic action. It is known to promote initial wound healing and this may be especially beneficial to applications involving diabetic patients and soft tissue healing. Bioactive glass scaffolds were fabricated using the polymer foam replication method. In vitro degradation and bioactivity of the scaffolds were evaluated in simulated body fluid under static conditions. Results revealed that the vanadium-containing borate glasses have higher degradation rates compared to the bare borate glass 13-93B3. Due to low chemical durability, substituted glasses exhibited a good in vitro bioactive response. Taking into account the high in vitro HA forming ability, borate glass scaffolds containing V+5therapeutic ions are promising candidates for bone tissue engineering applications. © 2014 W. S. Maney & Son Ltd.Item Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis virus(Elsevier Ltd, 2014) Tiwari P.M.; Eroglu E.; Bawage S.S.; Vig K.; Miller M.E.; Pillai S.; Dennis V.A.; Singh S.R.Reduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery, bioimaging and theranostics by conjugating cell-penetrating peptides (CPPs). This study presents the biodistribution and enhanced intracellular uptake of GNPs functionalized with VG-21, a CPP derived from vesicular stomatitis virus glycoprotein (G). Cell penetrating efficiency of VG-21 was demonstrated using CellPPD web server, conjugated to GNPs and were characterized using, UV-visible and FTIR spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential. Uptake of VG-21 functionalized GNPs (fGNPs) was tested in eukaryotic cell lines, HEp-2, HeLa, Vero and Cos-7, using flow cytometry, fluorescence and transmission electron microscopy (TEM), and inductively coupled plasmon optical emission spectroscopy (ICP-OES). The effects of nanoparticles on stress and toxicity related genes were studied in HEp-2cells. Cytokine response to fGNPs was studied invitro and invivo. Biodistribution of nanoparticles was studied in BALB/c mice using TEM and ICP-OES. VG-21, GNPs and fGNPs had little to no effect on cell viability. Upon exposure to fGNPs, HEp-2cells revealed minimal down regulation of stress response genes. fGNPs displayed higher uptake than GNPs in all cell lines with highest internalization by HEp-2, HeLa and Cos-7cells, in endocytotic vesicles and nuclei. Cytokine ELISA showed that mouse J774cells exposed to fGNPs produced less IL-6 than did GNP-treated macrophage cells, whereas TNF-α levels were low in both treatment groups. Biodistribution studies in BALB/c mice revealed higher accumulation of fGNPs than GNPs in the liver and spleen. Histopathological analyses showed that fGNP-treated mice accumulated 35ng/mg tissue and 20ng/mg tissue gold in spleen and liver respectively, without any adverse effects. Likewise, serum cytokines were low in both GNP- and fGNP-treated mice. Thus, VG-21-conjugated GNPs have enhanced cellular internalization and are suitable for various biomedical applications as nano-conjugates. © 2014 The Authors.Item Evaluation of borate bioactive glass scaffolds with different pore sizes in a rat subcutaneous implantation model(2014) Deliormanli A.M.; Liu X.; Rahaman M.N.Borate bioactive glass has been shown to convert faster and more completely to hydroxyapatite and enhance new bone formation in vivo when compared to silicate bioactive glass (such as 45S5 and 13-93 bioactive glass). In this work, the effects of the borate glass microstructure on its conversion to hydroxyapatite (HA) in vitro and its ability to support tissue ingrowth in a rat subcutaneous implantation model were investigated. Bioactive borate glass scaffolds, designated 13-93B3, with a grid-like microstructure and pore widths of 300, 600, and 900 μm were prepared by a robocasting technique. The scaffolds were implanted subcutaneously for 4 weeks in Sprague Dawley rats. Silicate 13-93 glass scaffolds with the same microstructure were used as the control. The conversion of the scaffolds to HA was studied as a function of immersion time in a simulated body fluid. Histology and scanning electron microscopy were used to evaluate conversion of the bioactive glass implants to hydroxyapatite, as well as tissue ingrowth and blood vessel formation in the implants. The pore size of the scaffolds was found to have little effect on tissue infiltration and angiogenesis after the 4-week implantation. © The Author(s) 2012 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.Item Preparation and in vitro characterization of electrospun 45S5 bioactive glass nanofibers(Elsevier Ltd, 2015) Deliormanli A.M.Bioactive glasses are widely used in biomedical applications due to their ability to bond to bone and even to soft tissues. In this study, 45S5 bioactive glass fibers were prepared through sol-gel processing and electrospinning technique. A precursor solution containing poly vinyl alcohol and bioactive glass sol was used to produce fibers. The mixture was electrospun at a voltage of 20 kV by maintaining tip to a collector distance of 8 cm. The fibers with an average diameter of 337781 nm (before calcination) were successfully obtained. Results showed that the crystalline phase of the fibers was largely influenced by the calcination temperature. Hydroxyapatite formation on calcined 45S5 fibers was investigated in simulated body fluid (SBF) using different fiber/SBF (F/S) ratios (0.5, 1, 2 and 10 mg/ml) at 37 °C. When immersed in SBF, conversion to a calcium phosphate material showed a strong dependence on the F/S ratio. At high solid concentration (10 mg/ml), surface of the fibers could not be converted to the HA-like material in SBF after 30 days. At lower solid concentrations (2, 1 and 0.5 mg/ml) an amorphous calcium phosphate layer formation was observed followed by the conversion to hydroxyapatite. © 2014 Elsevier Ltd and Techna Group S.r.l. All rights reserved.Item Synthesis and characterization of cerium- and gallium-containing borate bioactive glass scaffolds for bone tissue engineering(Kluwer Academic Publishers, 2015) Deliormanlı A.M.Bioactive glasses are widely used in biomedical applications due to their ability to bond to bone and even to soft tissues. In this study, borate based (13-93B3) bioactive glass powders containing up to 5 wt% Ce2O3 and Ga2O3 were prepared by the melt quench technique. Cerium (Ce+3) and gallium (Ga+3) were chosen because of their low toxicity associated with bacteriostatic properties. Bioactive glass scaffolds were fabricated using the polymer foam replication method. In vitro degradation and bioactivity of the scaffolds were evaluated in SBF under static conditions. Results revealed that the cerium- and gallium-containing borate glasses have much lower degradation rates compared to the bare borate glass 13-93B3. In spite of the increased chemical durability, substituted glasses exhibited a good in vitro bioactive response except when the Ce2O3 content was 5 wt%. Taking into account the high in vitro hydroxyapatite forming ability, borate glass scaffolds containing Ce+3 and Ga+3 therapeutic ions are promising candidates for bone tissue engineering applications. © 2015, Springer Science+Business Media New York.Item Fabrication and characterization of poly(ε-caprolactone) coated silicate and borate-based bioactive glass composite scaffolds(SAGE Publications Ltd, 2016) Deliormanli A.M.In this study, silicate 13-93 and borate based 13-93B3 bioactive glass scaffolds with high porosity and interconnected pore structure (pore size 100-500 μm) were prepared by foam replication method. In order to improve the mechanical properties, the scaffolds were coated and infiltrated with a poly(ε-caprolactone) (PCL) solution at different concentrations (5, 10, and 20 wt%). Results revealed that the mechanical properties of the scaffolds were significantly improved by the PCL coating. The addition of 10% PCL coating led to approximately 10-fold increase of compressive strength in comparison with noncoated scaffolds. The bioactivity of scaffolds upon immersion in simulated body fluid was maintained in the PCL-coated scaffolds at all concentrations; however, a decrease in the formation rate and amount of crystalline hydroxyapatite was observed as the PCL concentration was increased in the coating layer. Degradation rate of the borate-based bioactive glass scaffolds was tailored by the PCL coating. It is concluded that the fabricated bioactive composite scaffolds represent promising candidates for bone tissue engineering applications. © SAGE Publications.Item In vivo evaluation of cerium, gallium and vanadium-doped borate-based bioactive glass scaffolds using rat subcutaneous implantation model(Elsevier Ltd, 2016) Deliormanlı A.M.; Seda Vatansever H.; Yesil H.; Özdal-Kurt F.The main objective of this study was to evaluate the cerium, gallium and vanadium-containing bioactive borate glass scaffolds for soft tissue applications and determine the potential toxicity of these scaffolds on the adjacent tissues. The effects of the cerium, gallium and vanadium substitution on the soft tissue ingrowth and angiogenesis in porous borate based bioactive glass scaffolds were investigated using rat subcutaneous implantation model. For this purpose, bioactive borate glass powders containing therapeutic ions were prepared by melt-cast method and subsequently scaffolds were fabricated using polymer foam replication technique. The scaffolds were implanted subcutaneously for 4 weeks in Sprague Dawley rats. Bare borate glass scaffolds with the same microstructure were used as the control. Histology was used to evaluate tissue ingrowth and blood vessel formation in the implants. Additionally, the antibacterial activities of cerium, gallium and vanadium containing porous bioactive glass scaffolds were investigated in vitro by a zone inhibition method. Results revealed that addition of cerium ions to the borate glass network caused an increase in blood vessel formation. On the other hand, a decrease was obtained in angiogenesis in gallium and vanadium-containing glasses. All of the scaffolds prepared in the study did not show any antibacterial activity towards Escherichia coli and Staphylococcus aureus. © 2016 Elsevier Ltd and Techna Group S.r.l.Item Electrospun cerium and gallium-containing silicate based 13-93 bioactive glass fibers for biomedical applications(Elsevier Ltd, 2016) Deliormanlı A.M.Fibrous scaffolds have gained increasing attention in tissue engineering applications because of their high porosity, and surface area. In the current study cerium or gallium- containing silicate based 13-93 bioactive glass fibers were prepared using electrospinning approach. Effect of cerium and gallium substitution (up to 5 w%) on the in vitro bioactivity, pre-osteoblast cell response and antibacterial activity of the prepared fibers were assessed. Results revealed that, addition of cerium or gallium has no negative effect on bioactivity and hydroxyapatite forming ability of the glass in long term. The biocompatibility tests on MC3T3-E1 cells using XTT assay revealed no cytotoxicity of the cerium- or gallium-containing bioactive glass fibers. The fibers prepared in the study showed no antibacterial response to the gram positive (Staphylococcus aureus) and gram negative (Escherichia coli) bacteria. Fibrous scaffolds manufactured in the study may be considered for the use in soft tissue engineering applications in near future. © 2015 Elsevier Ltd and Techna Group S.r.l.Item Matrix changes due to the toxic effects of metronidazole in intestinal tissue of fish (Onchorhynchus mykiss)(Elsevier Ltd, 2016) Gürcü B.; Koca Y.B.; Özkut M.; Tuğlu M.T.Metranidazole (MTZ) is an antibiotic used for parasitic infections in a number of species. Accumulation of this drug in the environment and its interaction with fish of economic value makes this drug particularly important. In the present study, we examined the histopathological effects of MTZ on the intestinal tissue of Oncorhynchus mykiss.The fish in aquarium were exposed to MTZ at doses of 5, 10, 20 mg/L for 2, 4 and 8 days. At the end of the experiments, macroscopic pathology or death were not observed at these doses. Histochemical staining with Haematoxylene-Eosin, Periodic Acid Schiff and Gomori Trichrome showed, depending on increased dose and prolonged duration, areas of necrosis, edema, inflammation, small tears at the tips of the villi and excretion with heterogenic distribution of the Goblet cells. Moreover, changes in the connective tissue of the intestines due to toxicity of MTZ and decreases in immunostaining of matrix proteins such as laminin and collagen IV, especially in the epithelium were observed.Findings of the present study would be useful to demonstrate the adverse effects of MTZ use, emphasizing the importance of the effect on fish which could be very important public health. © 2015 Elsevier Ltd.Item Development of electromagnetic field system for providing wound healing on created wound model rat skin and sensor application; [Sicąn Derisinde Olusturulan Yara Modeli Üzerinde Iyilesme Saglanmasi için Elektromanyetik Alan Sistemi Gelistirilmesi ve Sensör Uygulamasi](Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Aydemir I.; Saygili S.; Kaya A.; Tuglu M.I.Electromagnetic field (EMF) radiation are formed by the acceleration of electric charge. Electric charges produce electric and magnetic energy in space when they move. These waves have different frequencies and wavelengths and when they interact with an object they can be refracted, reflected or absorbed. When a suitable frequency and waveform EM signal is applied to the target tissue with the help of a coil or applicator physiological effects will occur at the cellular size. Today studies revealed significant molecular mechanisms occurring during wound healing. Non-ionizing part of the EM spectrum is low-frequency electromagnetic fields are used as adjunct therapy in soft tissue injuries. In this study, the main goal is development of systems which will give better results compared with conventional treatment methods taking into account the effect on rat skin. To achieve this, some improvements in output power, increased gain, versatility, easy adjustments are provided for the spiral and planar antenna-applicator design. © 2016 The Chamber of Turkish Electrical Engineers.Item Electrically conductive borate-based bioactive glass scaffolds for bone tissue engineering applications(SAGE Publications Ltd, 2017) Turk M.; Deliormanll A.M.In this study, electrically conductive, borate-based, porous 13-93B3 bioactive glass composite scaffolds were prepared using a polymer foam replication technique. For this purpose, a slurry containing 40 vol% glass particles and 0-10 wt% graphene nanoplatelets was prepared by dispersing the particles in ethanol in the presence of ethyl cellulose. Composite scaffolds were subjected to a controlled heat treatment, in air atmosphere, to decompose the foam and sinter the glass particles into a dense network. It was found that the applied heat treatment did not influence the structure of graphene in the glass network. Graphene additions did not negatively affect the mechanical properties and enhanced the electrical conductivity of the glass scaffolds. In X-ray diffraction analysis, the crystalline peak corresponding to hydroxyapatite was observed in all the samples suggesting that all of the samples were bioactive after 30 days of immersion in simulated body fluid. However, Fourier transform infrared spectroscopy analysis and scanning electron microscope observations revealed that hydroxyapatite formation rate decreased with increasing graphene concentration especially for samples treated in simulated body fluid for shorter times. Based on the cytotoxicity assay findings, the MC3T3-E1 cell growth was significantly inhibited by the scaffolds containing higher amount of graphene compared to bare glass scaffolds. Best performance was obtained for 5 wt% graphene which yielded an enhancement of electrical conductivity with moderate cellular response and in vitro hydroxyapatite forming ability. The study revealed that the electrically conductive 13-93B3 graphene scaffolds are promising candidates for bone tissue engineering applications. © The Author(s) 2017.Item Investigation of in vitro mineralization of silicate-based 45S5 and 13-93 bioactive glasses in artificial saliva for dental applications(Elsevier Ltd, 2017) Deliormanlı A.M.Bioactive glasses are important class of materials that have a wide range of applications in tissue engineering and dentistry. In dental tissue engineering, nanofibrous structures exhibit interesting features, such as high surface area, surface functionalization and porosity. In this study, silicate-based 45S5 and 13-93 bioactive glass fibers were fabricated using electrospinning technique and their in vitro mineralization behavior was investigated in two different artificial saliva solutions for various time intervals. Results revealed that both 45S5 and 13-93 bioactive glass fibers show high mineralization behavior in artificial saliva solutions. However different hydroxyapatite (HA) formation rates were observed depending on the glass type and the artificial saliva composition. HA formation initiated earlier in 45S5 glass fibers treated in artificial saliva compared to 13-93 glass. On the other hand, after 30 days of treatment, the surface of 13-93 glass fibers converted to pure crystalline HA, whereas, 45S5 glass surface contained some additional crystalline phases such as aragonite and calcite. Treatment in SAGF medium resulted with better HA conversion ability compared to Carter-Brugirard saliva for both types of glass fibers. In conclusion, the use of electrospun nanofibrous 45S5 and 13-93 bioactive glass scaffolds could be one approach suitable to dental applications. © 2016 Elsevier Ltd and Techna Group S.r.l.Item Design of antenna-applicator for electromagnetic field applications and excitation model for rat and heat transfer analysis; [Elektromanyetik Alan Uygulamalari için Anten-Aplikatör Tasarimi, Sicąn için Uyartim Modeli ve Isi Daglimi Analizi](Institute of Electrical and Electronics Engineers Inc., 2017) Gumusay M.; Gulbagca F.; Aydemir I.; Saygili S.; Kaya A.; Tuglu M.I.There has been only a few technical progress which contribute to wound healing in the last century. Despite these advances wound healing is very hard issue to study because of its subjectivity, diversity and complexity of the wound healing process and diversity of patients. Molecules are insufficient in the chronic wound and unlike other cells multiplying endothelial cells interrupt the healing process. To restart this process, cells can be excitated by pulsed electromagnetic fields stimulation method. When a suitable frequency and waveform electromagnetic field (EMF) signal is applied to the target tissue with the help of a coil or applicator physiological effects occurs at the cellular size. Low-frequency electromagnetic fields are non-ionizing part of the EM spectrum and they are used as adjunct therapy in soft tissue injuries. In this study, an antenna-applicator design was performed and the effect of non-thermal pulsed radio frequency energy (PRFE) system to the wound created rat skin was examined. © 2016 The Chamber of Turkish Electrical Engineers.