Browsing by Subject "mannose binding lectin"

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    LY96, UPKIB mutations and TLR4, CD14, MBL polymorphisms in children with urinary tract infection
    (Springer, 2011) Ertan P.; Berdeli A.; Yilmaz O.; Gonulal D.A.; Yuksel H.
    Objective: To evaluate genetic variations of innate immune system such as mannose binding lectin (MBL), Toll like receptor 4 (TLR4), CD14, LY96 (MD2) and Uroplakin 1B (UPK1B) genes in children with recurrent urinary tract infection (UTI). Methods: The study included 30 children with recurrent UTI and 30 healthy controls. Blood was drawn and analysed for genetic polymorphisms of MBL, TLR4 and CD14 genes by the PCR-RFLP method. Direct DNA sequencing analysis was performed for LY96 and UPK 1B gene mutation in 10 children from UTI group and 5 children from control group. Results: TLR4 gene Thr399Ile polymorphism was not observed in any child. Genotype distribution and allele frequency of Asp299Gly polymorphism was similar in both groups (p∈=∈0.55). Codon 54 polymorphism of the MBL gene was similar in UTI and control groups (p∈=∈0.49). -159 CC/CT/TT genotypes of CD14 gene was similar between the two groups (p∈=∈0.14). UPK1B and LY96 gene DNA sequence analysis was similar in UTI and control groups. Conclusions: This study is the first study in which different parts of the innate immune system were evaluated in UTI etiopathogenesis in Turkish children. The results did not point out a significant role of any of the genes evaluated in this study. © 2011 Dr. K C Chaudhuri Foundation.
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    Mannose binding lectin (MBL) gene polymorphism and relationship between serum MBL concentrations in COPD patients; [KOAH hastalarında mannoz baǧlayan lektin (MBL) gen polimorfizmi ve serum MBL derişimi arasındaki ilişki]
    (Turkish Biochemistry Society, 2012) Ulutas G.S.; Taneli F.; Alpaydin A.O.; Cetinkaya C.; Ulman C.; Guvenc Y.; Dinc G.; Coskun A.S.
    Aim: We aimed to assess mannose-binding lectin (MBL) gene polymorphisms and serum MBL concentrations in a sample of Turkish chronic obstructive pulmonary disease (COPD) patients as well as in cigarette smokers. Furthermore, we looked for the possible correlations of serum MBL concentrations with pulmonary function tests. Materials and methods: Forty COPD patients and 40 healthy volunteers were included. The subjects were thereafter divided into 2 groups according to smoking status. Circulating MBL concentrations were assessed by ELISA and MBL gene polymorphisms were assessed by real time PCR method. Spirometry was performed to all subjects except healthy nonsmokers. Results: In the whole study population MBL gene frequencies were found 82.5%(66/80) for A/B genotype, 15%(12/80) for D/D genotype and 2.5%(2/80) for B/B genotype. Circulating MBL concentrations were found 2103±1311 ng/ml and 2324±1001 ng/ml in smoker and nonsmoker COPD patients, respectively, whereas they were 1746±1142 ng/ml in smoker and 2040±879 ng/ml in nonsmoker controls. No statistical difference was found between the study groups for serum MBL concentrations. Serum MBL concentration correlated positively with cigarette smoking (r=0.280, p=0.030) and negatively with pulmonary functions (FEV1 (r=- 0.246, p=0.058). Conclusion: To our knowledge, no previous study has been performed in healthy Turkish population to detect the MBL gene polymorphisms. A/B genotype was the most frequent MBL variant in our study population; however serum MBL concentrations were not found compatible with MBL deficiency. We believe these results need further investigation which includes larger series to evaluate whether serum MBL concentration is a risk factor for COPD. © TurkJBiochem.com.