Browsing by Subject "recombinant epidermal growth factor"

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    The effect of human recombinant epidermal growth factor on capsule contraction in an irradiated rat model
    (Springer Science and Business Media Deutschland GmbH, 2023) Tuluy Y.; Evrenos M.K.; Yoleri L.; Temiz P.; Ölmezoğlu A.
    Background: Breast implants are commonly used in plastic surgery for aesthetic and reconstructive purposes. One of the most important complications of this surgery is capsule contraction. Methods: In this study, it is aimed to evaluate the effect of recombinant human epidermal growth factor (rh-EGF) on capsule contraction in the model of capsule contraction provided by radiation therapy after silicone block placement in rats. The results were evaluated by histopathologic examination. Twenty-four rats were divided into 4 groups. In the group A, rats were followed for 63 days without radiation therapy and rh-EGF. In the groups B, C, and D, a total of 19.56 Gy radiation therapy was administrated in two fractions with an interval of 1 week. Forty-two days after the last radiation therapy, 3 doses of rh-EGF were given to the group C and 6 doses of rh-EGF to the group D with an interval of 1 day. Rats were sacrificed on the 63rd day of the study. Results: In the study, no statistically significant difference was observed between the groups in chronic inflammation, eosinophil, and inflammatory cell amount. Neutrophil levels, collagen amount, vascular proliferation, and capsule thicknesses were examined, and it was observed that they were significantly increased in the experimental groups compared to the control group. However, there was no significant difference between the experimental groups. Conclusions: Different rh-EGF doses were used in the study, and no significant effect was observed on capsule contraction. Different doses and durations may be considered to evaluate the effectiveness. Level of evidence: Not gradable © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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    The effect of human recombinant epidermal growth factor on neovascularization and pedicle division time in a rat interpolation flap model
    (Turkiye Klinikleri, 2024) Parspanci A.; Keçeci Y.; Temiz P.
    Background/aim: In practice, waiting 2–3 weeks for interpolation flaps pedicle division result in certain morbidities and discomfort for patient. The division time of flap pedicle depends on neovascularization from the recipient bed and includes wound healing stages. We aimed to investigate the effect of recombinant human epidermal growth factor (rhEGF) on the flap viability during early pedicle division. Materials and methods: Thirty-six rats were allocated to two main groups as control and study. A cranial based flap measuring 5 × 5 cm was elevated from the back, including all layers of the skin. While the cranial half of the defect was primarily closed, the flap was inset into the distal half. In the study group, a single dose of 20 µg EGF was injected into the recipient site and wound edges before the flap inset. The control group received no treatment. Each main group was divided into three subgroups based on pedicle division time of 8, 11 and 14 days. After pedicle division, each flap was monitored and photographed for 7 days, and histopathological samples were collected. Viable and necrotic areas were compared, and flaps were examined histopathologically. Results: The necrosis area in the study group on the 11th day was significantly lower than that in the control group. The fibroblastic activity, granulation tissue and neovascularization on the 8th day, the granulation tissue level on the 11th day, and the neovascularization level on the 14th day were significantly higher in the study groups. Conclusion: Following the application of EGF, the necrosis area decreased within the study group. Histopathological assessments revealed a statistically significant increase in parameters related to granulation tissue and fibroblastic activity, notably neovascularization, across all subgroups within the study. It was concluded that the use of EGF positively affected the neovascularization, and flaps could be divided earlier. © TÜBİTAK.