Neuroprotective effects of catechins in an experimental Parkinson’s disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects

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dc.contributor.authorÖzduran G.
dc.contributor.authorBecer E.
dc.contributor.authorVatansever H.S.
dc.contributor.authorYücecan S.
dc.date.accessioned2024-07-22T08:05:07Z
dc.date.available2024-07-22T08:05:07Z
dc.date.issued2022
dc.description.abstractObjectives: The aim of the study was to establish an in vitro Parkinson’s disease (PD) model and to investigate the cell viability, anti-inflammatory, anti-apoptotic and neuroprotective effects of catechin and EGCG in SK-N-AS and in vitro PD model cells. Method: SK-N-AS human neuroblastoma cells were used. To develop an in vitro PD model, SK-N-AS cells were exposed to 6-hydroxydopamine. Model control was performed after ELISA analysis of dopamine and α-synuclein levels in the culture medium. Catechin and EGCG were administered to SK-N-AS and in vitro PD model cells. Cell viability was measured using MTT assay and trypan blue staining. Anti-inflammatory and anti-apoptotic activities of catechin and EGCG were investigated by indirect immunocytochemistry using anti-TNF-α, anti-IL-1β and anti-caspase-3. Results: After 24 hours of 6-hydroxydopamine administration at 50 μM, higher αlfa-synuclein and lower dopamine levels were found in PD model than SK-N-AS cells. Cell viability was similar between SK-N-AS and in vitro PD model cells. Treatment with both bioactive components increased cell viability of in vitro PD model cells. Caspase-3 immunoreactivity was significantly reduced in SK-N-AS and PD model cells after EGCG administration, while it was decreased only in PD model cells after catechin administration. IL-1β staining intensity weakened after catechin administration in PD model cells, after EGCG administration in SK-N-AS cells. TNF-α staining intensity was similar in both cells. Conclusion: Catechin and EGCG increased cell viability in PD model neuron cells. Both components showed anti-apoptotic and anti-inflammatory effects. Catechin may be more effective in preventing damage to neurons PD. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.DOI-ID10.1080/01616412.2021.2024715
dc.identifier.issn01616412
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12977
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd.
dc.subjectAnti-Inflammatory Agents
dc.subjectApoptosis
dc.subjectCatechin
dc.subjectCell Survival
dc.subjectDopamine
dc.subjectHumans
dc.subjectNeuroprotective Agents
dc.subjectOxidopamine
dc.subjectParkinson Disease
dc.subjectTumor Necrosis Factor Inhibitors
dc.subject3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
dc.subjectalpha synuclein
dc.subjectc0567
dc.subjectcaspase 3
dc.subjectcatechin
dc.subjectdopamine
dc.subjecte4268
dc.subjectepigallocatechin gallate
dc.subjecth4381
dc.subjectinterleukin 1beta
dc.subjectm2128
dc.subjectoxidopamine
dc.subjectsuperoxide dismutase
dc.subjectt8532
dc.subjecttriton x 100
dc.subjecttrypan blue
dc.subjecttumor necrosis factor
dc.subjectantiinflammatory agent
dc.subjectcatechin
dc.subjectneuroprotective agent
dc.subjectoxidopamine
dc.subjecttumor necrosis factor inhibitor
dc.subjectantiapoptotic activity
dc.subjectantiinflammatory activity
dc.subjectArticle
dc.subjectcell culture
dc.subjectcell viability
dc.subjectclinical evaluation
dc.subjectcontrolled study
dc.subjectdopaminergic nerve cell
dc.subjectenzyme linked immunosorbent assay
dc.subjectexperimental parkinsonism
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectin vitro study
dc.subjectmorphology
dc.subjectMTT assay
dc.subjectnerve cell
dc.subjectneuroblastoma
dc.subjectneuroblastoma cell
dc.subjectneuroprotection
dc.subjectSK-N-AS cell line
dc.subjectstatistical analysis
dc.subjectapoptosis
dc.subjectcell survival
dc.subjectParkinson disease
dc.titleNeuroprotective effects of catechins in an experimental Parkinson’s disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects
dc.typeArticle

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