Immunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma

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dc.contributor.authorSanci M.
dc.contributor.authorDikis C.
dc.contributor.authorInan S.
dc.contributor.authorTurkoz E.
dc.contributor.authorDicle N.
dc.contributor.authorIspahi C.
dc.date.accessioned2024-07-22T08:20:06Z
dc.date.available2024-07-22T08:20:06Z
dc.date.issued2011
dc.description.abstractAngiogenic factors, such as vascular endothelial growth factor (VEGF), its receptors and epidermal growth factor receptor (EGF-R), are involved in increased progression in many carcinomas. The aim of this study was to investigate the role of angiogenesis and immunolocalization of VEGF, its receptors, EGF-R and Ki 67 in leiomyomas and leiomyosarcomas using an indirect immunohistochemical method. Samples from patients with leiomyoma, cellular leiomyoma and cellular leiomyosarcoma (n=20 per group) were fixed in 10% formalin and processed using routine paraffin protocols. Following initial histological analysis, samples were immunostained with primary antibodies for VEGF, VEGFR-1, VEGFR-2, EGF-R and Ki-67 using an indirect avidin-biotin peroxidase method. Immunostaining intensities were evaluated as mild, moderate or strong and a semi-quantitative method (H-Score) was used to compare the samples. While mild/moderate EGF-R immunostaining and moderate immunostaining for VEGF and its receptors were observed in samples of leiomyomas, much less immunoreactivity was observed in cellular leiomyomas. All immunoreactivities and immune-stained cells increased in leiomyosarcomas. When scores of intensity and percentage of positive staining cells were compared, all immunoreactivities were shown to be significantly increased in leiomyosarcomas compared to leiomyomas.These results suggest that in leiomyosarcoma, angiogenic factors, such as VEGF, its receptors and EGF-R, may be involved in tumor angiogenesis. Active tumor cells can trigger angiogenesis, interaction with surrounding tissue and in the tissue itself initiating angiogenic activity. Angiogenic growth factors play an important role and induce malignant transformation through both autocrine and paracrine mechanisms. Anti-angiogenic agents may provide a novel therapeutic approach for the treatment of leiomyosarcoma. © 2010 Elsevier GmbH.
dc.identifier.DOI-ID10.1016/j.acthis.2010.01.001
dc.identifier.issn16180372
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17974
dc.language.isoEnglish
dc.subjectAdult
dc.subjectAged
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectKi-67 Antigen
dc.subjectLeiomyoma
dc.subjectLeiomyosarcoma
dc.subjectMiddle Aged
dc.subjectNeovascularization, Pathologic
dc.subjectReceptor, Epidermal Growth Factor
dc.subjectReceptors, Vascular Endothelial Growth Factor
dc.subjectVascular Endothelial Growth Factor A
dc.subjectavidin
dc.subjectbiotin
dc.subjectepidermal growth factor receptor
dc.subjectKi 67 antigen
dc.subjectvasculotropin
dc.subjectvasculotropin receptor 1
dc.subjectvasculotropin receptor 2
dc.subjectadult
dc.subjectaged
dc.subjectarticle
dc.subjectautocrine effect
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectimmunolocalization
dc.subjectimmunoreactivity
dc.subjectleiomyoma
dc.subjectleiomyosarcoma
dc.subjectmajor clinical study
dc.subjectmalignant transformation
dc.subjectparacrine signaling
dc.subjecttissue interaction
dc.subjecttumor cell
dc.subjecttumor vascularization
dc.titleImmunolocalization of VEGF, VEGF receptors, EGF-R and Ki-67 in leiomyoma, cellular leiomyoma and leiomyosarcoma
dc.typeArticle

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