The effect of DEBIO 1143 usage alone or in combination with tamoxifen on estrogen receptor positive breast cancer cell lines; [DEBIO 1143’ün tek başına veya tamoxifenle kombinasyonunun östrojen reseptörü pozitif meme kanseri hücre hatlarındaki etkisi]
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Date
2020
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Abstract
Purpose: To investigate the effect of increased concentrations of tamoxifen (TAM) and DEBIO 1143 (AT-406) administered alone or in combination on cells in MCF-7 and BT-474 estrogen receptor positive (ER+) breast cancer cell lines. Materials and methods: The effect of tamoxifen and DEBIO 1143 administered alone or in combination on cell viability in MCF-7 and BT-474 cell lines at the 72nd hour was assessed by the XTT test. Multi-parameter apoptosis assay kit was used to display the effect of the alone/combination of TAM and DEBIO 1143 on both cell lines. Fluorescence microscobic analysis was performed. Results: The IC50 value of TAM was 3.8±0.6 micromolar (μΜ) and 18.9±6.7 μΜ in the MCF-7 and BT-474 cell lines, respectively. The IC50 value of DEBIO 1143 was 15±0.5 μM in the MCF-7 cell line. The results related to drug combination were statistically significant for both cell lines (p<0.001). The decrease in cell viability was not associated with apoptosis. Conclusion: In ER+breast cancer cell lines, the combined doses of the TAM and DEBIO 1143 reduced cell viability more than their administration alone. Combined administrations in both cell lines were concluded in a synergistic effect. Further research is needed to determine which cell death type other than apoptosis is associated with a reduction in cell viability caused by combined administration. © 2020, Pamukkale University. All rights reserved.