Investigation of poly-β-hydroxybutyrate (PHB) production by Bacillus subtilis ATCC 6633 Under different conditions; [Farklı koşullar altında Bacillus subtilis ATCC 6633 tarafından poli-β-hidroksibütirat (PHB) üretiminin araştırılması]
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Date
2011
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Abstract
The aim of our study was to assess the PHB productivity of Bacillus subtilis ATCC 6633 strain under different conditions. PHB amount was measured by UV-VIS spectrophotometer at 235 nm. The effects of the incubation time, temperature, pH, carbon sources, nitrogen sources and the rate of carbon/nitrogen ratio on PHB production rate were investigated. The results demonstrated that the highest PHB production was obtained after 24 h of incubation (10.4981 μg/ml), at 7.0 of pH (10.4981 μg/ml), with D-mannitole (23.6623 μg/ml) as carbon source, L-glycine (14.6217 μg/ml) as nitrogen source, C/N ratio of 2.5 (3.2481 μg/ml) and with a temperature of 30°C in culture media (10.4981 μg/ml).