T030 is the most common spa type among methicillin-resistant staphylococcus aureus strains isolated from Turkish hospitals; [T030, Türkiye'deki hastanelerden izole edilen metisiline dirençli staphylococcus aureus izolatlari arasinda en yaygin spa tipidir]

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dc.contributor.authorBozdoǧan B.
dc.contributor.authorYildiz Ö.
dc.contributor.authorOryaşin E.
dc.contributor.authorKirdar S.
dc.contributor.authorGülcü B.
dc.contributor.authorAktepe O.
dc.contributor.authorArslan U.
dc.contributor.authorBayramoǧlu C.
dc.contributor.authorÇoban A.Y.
dc.contributor.authorCoşkuner S.A.
dc.contributor.authorGüdücüoǧlu H.
dc.contributor.authorKarablber N.
dc.contributor.authorÖncü S.
dc.contributor.authorTatman Otkun M.
dc.contributor.authorÖzkütük N.
dc.contributor.authorÖzyurt M.
dc.contributor.authorŞener A.G.
dc.date.accessioned2024-07-22T08:18:40Z
dc.date.available2024-07-22T08:18:40Z
dc.date.issued2013
dc.description.abstractStaphylococcus aureus is one of the most frequent agents causing hospital infections. S.aureus has a great ability to adapt itself to variety of conditions and successful clones can be epidemic and even pandemic by its ability spread from one continent to another. The aims of this study were to detect spa types of 397 methicillin-resistant S.aureus (MRSA) strains isolated from 12 centers in different geographical regions of Turkey from 2006 to 2008, and to investigate their clonality by PFCE and MLST typing. Additionally, 91 MRSA from four of those 12 centers isolated during 2011 were also studied for their spa types. PFGE profiles indicated the presence of a major pulsotype, namely pulsotype A with a rate of 91.4% (363/397), followed by pulsotype B (n= 18, 4.5%) and pulsotype C (n= 11, 2.8%). Among isolates tested 363 (91.4%) were SCCmec type III, 30 (7.6%) were SCCmec type IV. Sequence analysis of representative isolates revealed that ST239 (85.1%) was the most common MLST type followed by two MLST types ST737 (4%), and ST97 (2.8%), both SCCmec type IV. Two isolates were ST80 with SCCmec type IV. Of 397 isolates, 338 (85.1%) were t030, followed by t005 (2.5%) and t632 (2%). Among MRSA isolated during 2011, 64 (70.3%) of 91 were t030, 4 (4.4%) were tOOS, 2 (2.2%) were t015, and 2 (2.2%) were t1094. Among centers the t030 prevalence of 2006-2008 isolates ranged from 59-100%. The highest t030 prevalence was found in Ankara (100%) and lowest in Trabzon (59%) provinces which are located at central and northestern Anatolia, respectively. In Istanbul province, the prevalence of t030 was 94.5% among 2006-2008 isolates which decreased to 55.5% among 2011 isolates. Also a decrease in t030 rates was observed among samples from Konya and Trabzon but not from Aydin. Our results showed that the most common MRSA clone in Turkey is ST 239-SCCmec type III, t030 which persisted during the six years of the study period. Presence of PVL toxin gene was tested by PCR and 5 (3%) isolates found to be positive, of them two were SCCmec Type IV-ST80 and three were SCCmec Type III-ST239. This study is the largest epidemiological survey ever done in Turkey which showed presence of a hospital Turkish clone TR09 (ST239-SCC meclll-t030) and a community clone TR10 (ST737-SCCmedV-t005) largely disseminated in Turkey.
dc.identifier.DOI-ID10.5578/mb.5770
dc.identifier.issn03749096
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17375
dc.language.isoTurkish
dc.publisherAnkara Microbiology Society
dc.rightsAll Open Access; Bronze Open Access
dc.subjectBacterial Toxins
dc.subjectCross Infection
dc.subjectDNA, Bacterial
dc.subjectElectrophoresis, Gel, Pulsed-Field
dc.subjectExotoxins
dc.subjectHumans
dc.subjectLeukocidins
dc.subjectMethicillin-Resistant Staphylococcus aureus
dc.subjectMultilocus Sequence Typing
dc.subjectPolymerase Chain Reaction
dc.subjectPrevalence
dc.subjectStaphylococcal Infections
dc.subjectTurkey
dc.subjectbacterial DNA
dc.subjectbacterial toxin
dc.subjectexotoxin
dc.subjectleukocidin
dc.subjectPanton Valentine leukocidin
dc.subjectPanton-Valentine leukocidin
dc.subjectarticle
dc.subjectclassification
dc.subjectcross infection
dc.subjectgenetics
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmethicillin resistant Staphylococcus aureus
dc.subjectmicrobiology
dc.subjectmulticenter study
dc.subjectmultilocus sequence typing
dc.subjectpolymerase chain reaction
dc.subjectprevalence
dc.subjectpulsed field gel electrophoresis
dc.subjectStaphylococcus infection
dc.subjectTurkey (republic)
dc.titleT030 is the most common spa type among methicillin-resistant staphylococcus aureus strains isolated from Turkish hospitals; [T030, Türkiye'deki hastanelerden izole edilen metisiline dirençli staphylococcus aureus izolatlari arasinda en yaygin spa tipidir]
dc.typeArticle

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