In vivo study to evaluate the protective effects of amifostine on radiation-induced damage of testis tissue

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dc.contributor.authorAndrieu M.N.
dc.contributor.authorKurtman C.
dc.contributor.authorHicsonmez A.
dc.contributor.authorOzbilgin K.
dc.contributor.authorEser E.
dc.contributor.authorErdemli E.
dc.date.accessioned2024-07-22T08:23:52Z
dc.date.available2024-07-22T08:23:52Z
dc.date.issued2005
dc.description.abstractObjective: To investigate the early protective effects of amifostine against radiation-induced damage on rat testis tissue. Methods: Eighty adult male Wistar rats were randomized to 4 groups: Saline solution was given to group A for control, 200 mg/kg amifostine (WR-2721) to group B, a single fraction of 6 Gy local irradiation to testes in group C and 200 mg/kg amifostine 15-30 min before 6 Gy testicular irradiation to group D. Animals were sacrificed 3 weeks after treatment and their testes were removed for macroscopic, microscopic and ultrastructural histopathological examination. Results: The weights, widths and lengths of testes in the lasts groups had decreased significantly when compared with the control group, but the decrease in widths after irradiation was found to be significantly less only in the amifostine plus radiation group. There was a significant reduction of testis weights in relation to the individual body weights in the irradiated testes compared with the other groups (p < 0.005), while there was no significant change of testis weight/total body weight ratio in amifostine plus irradiation group. Spermatogonium A and primary spermatocyte counts were also less in the treatment groups, and primary spermatocyte numbers were significantly higher in amifostine plus radiation group when compared with radiation alone group (p < 0.005). Pretreatment with amifostine reduced the decrease of primary spermatocyte counts by a factor of 1.28. Electron microscopic analysis did not show any cytotoxic effect of amifostine alone, and furthermore, ultrastructural findings were normal with the addition of amifostine prior to irradiation, though there was damage in the radiation exposure group. Conclusion: Amifostine when given alone by itself appears to cause adverse alterations in testis tissue; however, it has a radioprotective effect on spermiogenetic cells when used prior to radiation. Copyright © 2005 S. Karger AG.
dc.identifier.DOI-ID10.1159/000087475
dc.identifier.issn00302414
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19734
dc.language.isoEnglish
dc.subjectAmifostine
dc.subjectAnimals
dc.subjectIn Situ Nick-End Labeling
dc.subjectMale
dc.subjectMicroscopy
dc.subjectMicroscopy, Electron
dc.subjectRadiation Injuries
dc.subjectRadiation-Protective Agents
dc.subjectRandom Allocation
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSeminiferous Epithelium
dc.subjectSperm Count
dc.subjectTestis
dc.subjectamifostine
dc.subjectsodium chloride
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbody weight
dc.subjectcancer radiotherapy
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectdrug effect
dc.subjectelectron microscopy
dc.subjecthistopathology
dc.subjectmale
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectradiation dose fractionation
dc.subjectradiation injury
dc.subjectradiation protection
dc.subjectrat
dc.subjectspermatocyte
dc.subjectspermatogonium
dc.subjectspermatozoon count
dc.subjecttestis injury
dc.subjecttestis weight
dc.subjecttreatment outcome
dc.subjectultrastructure
dc.titleIn vivo study to evaluate the protective effects of amifostine on radiation-induced damage of testis tissue
dc.typeArticle

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