Defining the macroscopic and microscopic findings of experimental focal brain ischemia in rats from a forensic scientist's point of view

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dc.contributor.authorTatlisumak E.
dc.contributor.authorInan S.
dc.contributor.authorAsirdizer M.
dc.contributor.authorApaydin N.
dc.contributor.authorHayretdag C.
dc.contributor.authorKose C.
dc.contributor.authorTekdemir I.
dc.date.accessioned2024-07-22T08:21:52Z
dc.date.available2024-07-22T08:21:52Z
dc.date.issued2009
dc.description.abstractApproximately 10% of all deaths in the world occur as a result of stroke. Determination of the time schedule of the pathologic events in a stroke patient is invaluable for a forensic specialist. The aim of this study was to define the schedule of the macroscopic and microscopic changes that occurred in a rat model of permanent focal ischemia for providing useful clues for the evaluation of stroke patients. Male Wistar rats weighing 250 to 350 g were used in this study. Permanent focal brain ischemia was applied by the suture occlusion method. The animals were divided into 7 experimental groups (n ≤ 6) with time schedules including 1.5, 3, 6, 12, 24, 72 hours, and the sham. Brains were harvested at the end of the determined time schedule. Lesions in the frontoparietal cortex were evaluated macroscopically first and later hematoxylin eosin stained sections from the infarct core were investigated microscopically. Macroscopically, enlargement of the ipsilateral hemisphere was mild at 6 hour, apparent at 12 and 24 hours, and mild again at 72 hours. Microscopically, ischemic changes were apparent even at 1.5 hour. Red neurons and infiltration of the parenchyma with neutrophil leukocytes were observed at 12 hours. Pannecrosis and massive leukocyte infiltration were observed at 72 hours. Macroscopic and microscopic findings obtained from a rat model may provide clues for determination of the time-dependent changes due to brain ischemia in human subjects. Finally, the benefits of determination of time course of pathologic changes in the brain for forensic scientists were discussed. © 2009 by Lippincott Williams & Wilkins.
dc.identifier.DOI-ID10.1097/PAF.0b013e3181873c32
dc.identifier.issn01957910
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18815
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Ischemia
dc.subjectForensic Pathology
dc.subjectLeukocytes
dc.subjectMale
dc.subjectMicroscopy
dc.subjectModels, Animal
dc.subjectNeurons
dc.subjectNeutrophils
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStroke
dc.subjectTime Factors
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbrain ischemia
dc.subjectbrain region
dc.subjectcontrolled study
dc.subjectexperimental model
dc.subjectexperimental rat
dc.subjectexperimental study
dc.subjecthemisphere
dc.subjectimmunohistochemistry
dc.subjectinfarction
dc.subjectleukocyte
dc.subjectmale
dc.subjectmicroscopy
dc.subjectnerve cell
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectocclusion
dc.subjectparenchyma
dc.subjectparietal lobe
dc.subjectrat
dc.subjectsham procedure
dc.subjectstroke
dc.subjectsuture
dc.subjectWistar rat
dc.subjectanimal
dc.subjectbrain
dc.subjectbrain ischemia
dc.subjectforensic pathology
dc.subjectpathology
dc.subjectstroke
dc.subjecttime
dc.titleDefining the macroscopic and microscopic findings of experimental focal brain ischemia in rats from a forensic scientist's point of view
dc.typeArticle

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