Enhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis virus

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dc.contributor.authorTiwari P.M.
dc.contributor.authorEroglu E.
dc.contributor.authorBawage S.S.
dc.contributor.authorVig K.
dc.contributor.authorMiller M.E.
dc.contributor.authorPillai S.
dc.contributor.authorDennis V.A.
dc.contributor.authorSingh S.R.
dc.date.accessioned2024-07-22T08:16:59Z
dc.date.available2024-07-22T08:16:59Z
dc.date.issued2014
dc.description.abstractReduced toxicity and ease of modification make gold nanoparticles (GNPs) suitable for targeted delivery, bioimaging and theranostics by conjugating cell-penetrating peptides (CPPs). This study presents the biodistribution and enhanced intracellular uptake of GNPs functionalized with VG-21, a CPP derived from vesicular stomatitis virus glycoprotein (G). Cell penetrating efficiency of VG-21 was demonstrated using CellPPD web server, conjugated to GNPs and were characterized using, UV-visible and FTIR spectroscopy, transmission electron microscopy, dynamic light scattering and zeta potential. Uptake of VG-21 functionalized GNPs (fGNPs) was tested in eukaryotic cell lines, HEp-2, HeLa, Vero and Cos-7, using flow cytometry, fluorescence and transmission electron microscopy (TEM), and inductively coupled plasmon optical emission spectroscopy (ICP-OES). The effects of nanoparticles on stress and toxicity related genes were studied in HEp-2cells. Cytokine response to fGNPs was studied invitro and invivo. Biodistribution of nanoparticles was studied in BALB/c mice using TEM and ICP-OES. VG-21, GNPs and fGNPs had little to no effect on cell viability. Upon exposure to fGNPs, HEp-2cells revealed minimal down regulation of stress response genes. fGNPs displayed higher uptake than GNPs in all cell lines with highest internalization by HEp-2, HeLa and Cos-7cells, in endocytotic vesicles and nuclei. Cytokine ELISA showed that mouse J774cells exposed to fGNPs produced less IL-6 than did GNP-treated macrophage cells, whereas TNF-α levels were low in both treatment groups. Biodistribution studies in BALB/c mice revealed higher accumulation of fGNPs than GNPs in the liver and spleen. Histopathological analyses showed that fGNP-treated mice accumulated 35ng/mg tissue and 20ng/mg tissue gold in spleen and liver respectively, without any adverse effects. Likewise, serum cytokines were low in both GNP- and fGNP-treated mice. Thus, VG-21-conjugated GNPs have enhanced cellular internalization and are suitable for various biomedical applications as nano-conjugates. © 2014 The Authors.
dc.identifier.DOI-ID10.1016/j.biomaterials.2014.07.032
dc.identifier.issn01429612
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16943
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.subjectAnimals
dc.subjectCell Survival
dc.subjectCell-Penetrating Peptides
dc.subjectCercopithecus aethiops
dc.subjectCOS Cells
dc.subjectFemale
dc.subjectGold
dc.subjectHeLa Cells
dc.subjectHumans
dc.subjectInterleukin-6
dc.subjectMembrane Glycoproteins
dc.subjectMetal Nanoparticles
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMicroscopy, Electron, Transmission
dc.subjectTissue Distribution
dc.subjectTumor Necrosis Factor-alpha
dc.subjectVero Cells
dc.subjectVesiculovirus
dc.subjectViral Envelope Proteins
dc.subjectEukaryota
dc.subjectMus
dc.subjectVesicular stomatitis virus
dc.subjectCell culture
dc.subjectCytology
dc.subjectDrug delivery
dc.subjectElectromagnetic induction
dc.subjectElectron microscopy
dc.subjectEmission spectroscopy
dc.subjectFiber optic sensors
dc.subjectFourier transform infrared spectroscopy
dc.subjectGenes
dc.subjectGold
dc.subjectLight scattering
dc.subjectMammals
dc.subjectMedical applications
dc.subjectMetal nanoparticles
dc.subjectMobile security
dc.subjectNanoparticles
dc.subjectOptical emission spectroscopy
dc.subjectPeptides
dc.subjectTissue
dc.subjectTissue engineering
dc.subjectToxicity
dc.subjectTransmission electron microscopy
dc.subjectViruses
dc.subjectcell penetrating peptide
dc.subjectcytokine
dc.subjectdrug vehicle
dc.subjectgold nanoparticle
dc.subjectinterleukin 6
dc.subjectnanoconjugate
dc.subjectnanoparticle
dc.subjectprotein VG 21
dc.subjectprotein VG 21 gold nanoparticle conjugate
dc.subjecttumor necrosis factor alpha
dc.subjectunclassified drug
dc.subjectcell penetrating peptide
dc.subjectG protein, vesicular stomatitis virus
dc.subjectgold
dc.subjectinterleukin 6
dc.subjectmembrane protein
dc.subjectmetal nanoparticle
dc.subjecttumor necrosis factor alpha
dc.subjectvirus envelope protein
dc.subjectCell penetrating peptides (CPPs)
dc.subjectCell-penetrating peptide
dc.subjectCellular internalization
dc.subjectFunctionalized gold nanoparticles
dc.subjectGold nanoparticles (GNPs)
dc.subjectHistopathological analysis
dc.subjectIntracellular translocation
dc.subjectVesicular stomatitis virus
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectcell nucleus
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectCOS 7 cell line
dc.subjectcytokine production
dc.subjectdrug accumulation
dc.subjectdrug conjugation
dc.subjectdrug delivery system
dc.subjectdrug distribution
dc.subjectdrug effect
dc.subjectdrug transport
dc.subjectendosome
dc.subjectenzyme linked immunosorbent assay
dc.subjectflow cytometry
dc.subjectfluorescence microscopy
dc.subjectgene expression
dc.subjectHeLa cell line
dc.subjectHEp 2 cell line
dc.subjecthistopathology
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectinductively coupled plasmon optical emission spectroscopy
dc.subjectinfrared spectroscopy
dc.subjectinternalization
dc.subjectlight scattering
dc.subjectliver
dc.subjectmacrophage
dc.subjectmass spectrometry
dc.subjectmouse
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpriority journal
dc.subjectspleen
dc.subjecttissue distribution
dc.subjecttransmission electron microscopy
dc.subjectultraviolet spectroscopy
dc.subjectVero cell line
dc.subjectVesiculovirus
dc.subjectzeta potential
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectcell survival
dc.subjectchemistry
dc.subjectChlorocebus aethiops
dc.subjectCOS 1 cell line
dc.subjectdrug effects
dc.subjectfemale
dc.subjectmetabolism
dc.subjectVesiculovirus
dc.subjectCells
dc.titleEnhanced intracellular translocation and biodistribution of gold nanoparticles functionalized with a cell-penetrating peptide (VG-21) from vesicular stomatitis virus
dc.typeArticle

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