Inhibiting effect of oleocanthal on neuroblastoma cancer cell proliferation in culture

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dc.contributor.authorÜnsal Ü.Ü.
dc.contributor.authorMete M.
dc.contributor.authorAydemir I.
dc.contributor.authorDuransoy Y.K.
dc.contributor.authorUmur A.Ş.
dc.contributor.authorTuglu M.I.
dc.date.accessioned2024-07-22T08:07:33Z
dc.date.available2024-07-22T08:07:33Z
dc.date.issued2020
dc.description.abstractWe investigated the potential anticancer effects of oleocanthal (OC) on neuroblastoma cells. Cells were divided into four groups: group 1, neuroblastoma cells were treated with OC; group 2, neurons that differentiated from neuroblastoma cells were treated with phosphate-buffered saline(PBS); group 3, bone marrow derived neuronal (BMDN) cells that were differentiated from bone marrow derived mesenchymal stem cells (BMSCs) were treated with OC; group 4, BMDN cells that were differentiated from BMSCs were treated with PBS. Groups 2 and 4 were control groups. The effects of OC on cell viability, oxidative stress, neurite inhibition and apoptosis at IC50 dose were investigated using MTT analysis, i-NOS and e-NOS measurement, neurotoxicity screening test (NST) and TUNEL staining, respectively. MTT analysis demonstrated that cells were significantly less viable in group 1 than in group 3. i-NOS and e-NOS staining intensity was significantly greater in group 1 than in group 3. NST revealed that OC inhibited neurite growth in both neuroblastoma and BMND cells; inhibition was significantly less in group 3 than in group 1. Significantly more TUNEL labeled cells were found in group 1 than in group 3. We found that OC prevented growth and proliferation of neuroblastoma cells in culture by increasing oxidative stress and apoptosis. We also found that the cytotoxicity of OC is negligible in BMDN cells. © 2019, © 2019 The Biological Stain Commission.
dc.identifier.DOI-ID10.1080/10520295.2019.1674919
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13987
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAldehydes
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBone Marrow Cells
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCells, Cultured
dc.subjectCyclopentane Monoterpenes
dc.subjectMesenchymal Stem Cells
dc.subjectNeural Stem Cells
dc.subjectNeuroblastoma
dc.subjectPhenols
dc.subjectaldehyde
dc.subjectoleocanthal
dc.subjectphenol derivative
dc.subjectanimal
dc.subjectapoptosis
dc.subjectbone marrow cell
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcytology
dc.subjectdrug effect
dc.subjectmesenchymal stem cell
dc.subjectneural stem cell
dc.subjectneuroblastoma
dc.subjectpathology
dc.subjectpharmacology
dc.subjectphysiology
dc.titleInhibiting effect of oleocanthal on neuroblastoma cancer cell proliferation in culture
dc.typeArticle

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