Role of a combination dietary supplement containing mucopolysaccharides, vitamin C, and collagen on tendon healing in rats

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dc.contributor.authorGemalmaz H.C.
dc.contributor.authorSarıyılmaz K.
dc.contributor.authorOzkunt O.
dc.contributor.authorGurgen S.G.
dc.contributor.authorSilay S.
dc.date.accessioned2024-07-22T08:09:28Z
dc.date.available2024-07-22T08:09:28Z
dc.date.issued2018
dc.description.abstractObjective: The aim of this study was to investigate the effect of mucopolysaccharide, vitamin C, and collagen supplementation on the healing of Achilles tendon in rats. Methods: Sixteen rats were separated into 2 groups. Both Achilles tendons of all rats were transected 5 mm above the insertion and repaired using a Kessler suture. After the surgical repair, the study group received the daily recommended amount of the supplement by gastric gavage, while the control group received a placebo. At the end of the third week, the animals were sacrificed. The biomechanical properties of the groups were compared with ultimate tensile strength and stiffness tests. The biological properties of the 2 groups were assessed with a histomorphometric comparison to determine the amount of collagen type I (COL1), proliferating cell nuclear antigen (PCNA), and transforming growth factor β1 (TGF-β1) expression in 3 different tissue subgroups (collagen matrix, tenocytes, and endotenon fibroblasts). Results: Analysis of histomorphometric results revealed that the rats receiving dietary supplements demonstrated a significant increase in PCNA (mean value of 86 in the control group and 168.85 in the trial group; p < 0.05) and TGF-β1 (mean value of 87.57 in the control group and 161.85 in the trial group; p < 0.05) in the endotenon fibroblasts of the repair site. However, there was no difference between the groups in PCNA or TGF-β1 when the collagen matrix and the tenocytes of the repair site were examined. Furthermore, no significant difference could be found between groups in COL1 in any of the 3 tissue subgroups (collagen matrix, tenocytes, and endotenon fibroblasts). The statistical analysis also indicated that the rats receiving supplements did not demonstrate a significant increase in the ultimate tendon tensile strength or stiffness. Conclusion: The results of this study revealed no advantage to the oral administration of the trial supplement in collagen synthesis or biomechanical properties in rats after 3 weeks using the presented study design. However, the increased expression of PCNA and TGFβ1 seen in the endotenon fibroblasts of the repair site might play a role in the continuum of tendon healing. © 2018 Turkish Association of Orthopaedics and Traumatology
dc.identifier.DOI-ID10.1016/j.aott.2018.06.012
dc.identifier.issn1017995X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14837
dc.language.isoEnglish
dc.publisherTurkish Association of Orthopaedics and Traumatology
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAchilles Tendon
dc.subjectAnimals
dc.subjectAscorbic Acid
dc.subjectCollagen
dc.subjectDietary Supplements
dc.subjectDrug Combinations
dc.subjectGlycosaminoglycans
dc.subjectMale
dc.subjectProliferating Cell Nuclear Antigen
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectRegeneration
dc.subjectTendon Injuries
dc.subjectTensile Strength
dc.subjectTransforming Growth Factor beta1
dc.subjectTreatment Outcome
dc.subjectascorbic acid
dc.subjectcollagen
dc.subjectcycline
dc.subjectglycosaminoglycan
dc.subjecttransforming growth factor beta1
dc.subjectachilles tendon
dc.subjectanimal
dc.subjectdiet therapy
dc.subjectdietary supplement
dc.subjectdrug combination
dc.subjectmale
dc.subjectmetabolism
dc.subjectpathophysiology
dc.subjectphysiology
dc.subjectrat
dc.subjectregeneration
dc.subjectSprague Dawley rat
dc.subjecttendon injury
dc.subjecttensile strength
dc.subjecttreatment outcome
dc.titleRole of a combination dietary supplement containing mucopolysaccharides, vitamin C, and collagen on tendon healing in rats
dc.typeArticle

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