Induction of autophagic cell death by thymoquinone in docetaxel resistant prostate cancer cells; [Dosetaksel dirençli prostat kanseri hücrelerinde timokinon tarafından otofajik hücre ölümünün indüklenmesi]

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dc.contributor.authorİlhan S.
dc.contributor.authorOğuz F.
dc.date.accessioned2024-07-22T08:06:40Z
dc.date.available2024-07-22T08:06:40Z
dc.date.issued2021
dc.description.abstractAim: Acquired docetaxel (DOC) resistance of prostate cancer (PCa) is still a clinical problem. In addition to failure in chemotherapy treatment, it causes tumor recurrence. Therefore, novel and more effective compounds are needed in DOC-resistant PCa treatment. This study aimed to investigate the possible cytotoxic and cell death-inducing activities of thymoquinone (TQ), one of the main active components of Nigella sativa L., on DOC-resistant prostate cancer cells. Material and Methods: DOC-resistant PC3 cells (DOC-R/PC3) were developed by the continuous culture with increment concentrations of DOC (1-10 nM) until they improved their growth and division abilities. The cell viability was determined by MTT assay. The Muse™ Annexin V & Dead Cell kit was performed to detect apoptotic cell death. Autophagic vacuoles were observed by staining autophagic vacuoles. The levels of LC3I, LC3II and Beclin-1 proteins were investigated via western blot analysis. Results: TQ inhibited the viability of DOC-R/PC3 cells in a dose-and time-dependent manner (p=0.014). The IC50 value of TQ for DOC-R/PC3 cells was calculated as 60 µM at 72 h. Treatment of TQ did not induce apoptotic cell death in DOC-resistant prostate cancer cells but induced the formation of autophagic vacuoles. Moreover, Beclin-1 and LC3-II protein levels were increased in TQ-treated DOC-R/PC3 cells, however, LC3-I levels were decreased in DOC-R/PC3 cells. Conclusion: All these results show that TQ may become a new therapeutic target for DOC-resistant prostate cancer in the future. © 2021, Duzce University Medical School. All rights reserved.
dc.identifier.DOI-ID10.18678/dtfd.925238
dc.identifier.issn1307671X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13656
dc.language.isoEnglish
dc.publisherDuzce University Medical School
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectdocetaxel
dc.subjectglutamine
dc.subjectnefopam
dc.subjectphosphatidylserine
dc.subjectstreptomycin
dc.subjectthymoquinone
dc.subjectapoptosis
dc.subjectArticle
dc.subjectautophagic cell death
dc.subjectcell viability
dc.subjectcytotoxicity
dc.subjectfetal bovine serum
dc.subjecthuman
dc.subjecthuman cell
dc.subjectprostate cancer
dc.subjectprostate cancer cell line
dc.subjectprotein expression
dc.subjecttumor recurrence
dc.subjectWestern blotting
dc.titleInduction of autophagic cell death by thymoquinone in docetaxel resistant prostate cancer cells; [Dosetaksel dirençli prostat kanseri hücrelerinde timokinon tarafından otofajik hücre ölümünün indüklenmesi]
dc.typeArticle

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