Resveratrol modulates miRNA machinery proteins in different types of colon cancer cells

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dc.contributor.authorBecer E.
dc.contributor.authorMadencioğlu S.
dc.contributor.authorKabadayı H.
dc.contributor.authorVatansever H.S.
dc.date.accessioned2024-07-22T08:01:33Z
dc.date.available2024-07-22T08:01:33Z
dc.date.issued2024
dc.description.abstractObjectives: Resveratrol (RSV) is a stilbenoid compound that shows anticancer activity in many cancer cells. Exosomes might affect carcinogenesis and the development of colorectal cancer by affecting communication between tumor cells in the tumor microenvironment via their cargo content miRNA. The aim of this study is to determine the effect of RSV on the expression of Dicer, Ago2, eIf2α, CD-9, CD-63, and exosomal miRNA levels in COLO320 and COLO741 colon cancer cell lines. Methods: The MTT method was used for cell growth and cytotoxicity in both COLO320 and COLO741 cell lines. Dicer, Ago2, eIF2α, CD-9, and CD-63 antibodies were used for the immunocytochemical evaluation. Total miRNA analysis was performed using a miRCURY Exosome Isolation Kit. Results: As a result of immunocytochemical staining, increased CD-63 immunoreactivity was observed in RSV-treated COLO320 cells vs. RSV-treated COLO-741 cells. Dicer immunoreactivity increased after the RSV treatment in COLO320 cells. Higher eIF2α immunoreactivity was observed in RSV-treated COLO741 cells compared to both COLO741 control cells and RSV-treated COLO320 cells. Non-significant decreases were observed in miRNA concentration in RSV-treated COLO320 and COLO741 cells compared to control group cells. Conclusions: RSV could increase miRNA biogenesis in COLO320 cancer cells and decrease it in COLO741 cancer cells. © 2023 the author(s), published by De Gruyter.
dc.identifier.DOI-ID10.1515/tjb-2023-0076
dc.identifier.issn02504685
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11472
dc.language.isoEnglish
dc.publisherDe Gruyter Open Ltd
dc.rightsAll Open Access; Gold Open Access
dc.subjectmicroRNA
dc.subjectresveratrol
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcarcinogenesis
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectCOLO 320 cell line
dc.subjectcolon cancer
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectexosome
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectmicroscopy
dc.subjectMTT assay
dc.subjectprotein expression
dc.subjectsignal transduction
dc.subjecttumor microenvironment
dc.titleResveratrol modulates miRNA machinery proteins in different types of colon cancer cells
dc.typeArticle

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