Alteration in methylation pattern of retinoblastoma 1 gene promotor region in intestinal metaplasia with or without helicobacter pylori and gastric cancer patients

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dc.contributor.authorBoyacioglu S.O.
dc.contributor.authorKasap E.
dc.contributor.authorYuceyar H.
dc.contributor.authorKorkmaz M.
dc.date.accessioned2024-07-22T08:11:52Z
dc.date.available2024-07-22T08:11:52Z
dc.date.issued2016
dc.description.abstractBackground. Helicobacter pylori, intestinal metaplasia (IM), and gene methylation play important roles in gastric carcinogenesis. However, the association among H. pylori infection, IM, gastric cancer (GC), and gene methylation is not fully understood. Cell cycle control involving retinoblastoma 1 (RB1) gene is one of the main regulatory pathways reported to be altered in gastric carcinogenesis. Objectives. The purpose of this research is to assess the methylation status of RB1 gene in GC and IM with or without H. pylori infection, and to discuss the possible role of H. pylori-induced RB1 gene methylation in the mechanism of gastric carcinogenesis. Material and Methods. The methylation profile of RB1 gene was analyzed by sodium bisulfite modification and methylation-specific PCR in GC (n = 24), IM patients with H. pylori positive (n = 20) and negative (n = 20), and control subjects (n = 20). Results. According to methylation levels in RB1 gene; the high correlation values were detected between H. pylori positive-IM group and GC group, and between H. pylori positive-IM and H. pylori negative-IM groups (p < 0.05). No correlations between H. pylori negative-IM and GC groups and between GC and control groups were detected in methylation status of RB1 gene. Conclusions. High methylation levels in RB1 gene in H. pylori positive individuals may suggest an elevated risk of gastric cancer occurrence. © Copyright by Wroclaw Medical University.
dc.identifier.DOI-ID10.17219/acem/38842
dc.identifier.issn18995276
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15814
dc.language.isoEnglish
dc.publisherWroclaw University of Medicine
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAdult
dc.subjectAged
dc.subjectDNA Methylation
dc.subjectFemale
dc.subjectHelicobacter Infections
dc.subjectHelicobacter pylori
dc.subjectHost-Pathogen Interactions
dc.subjectHumans
dc.subjectIntestines
dc.subjectMale
dc.subjectMetaplasia
dc.subjectMiddle Aged
dc.subjectPolymerase Chain Reaction
dc.subjectPromoter Regions, Genetic
dc.subjectRetinoblastoma Protein
dc.subjectStomach Neoplasms
dc.subjectretinoblastoma protein
dc.subjectage
dc.subjectArticle
dc.subjectcancer patient
dc.subjectcancer risk
dc.subjectcarcinogenesis
dc.subjectcontrolled study
dc.subjectDNA methylation
dc.subjectDNA modification
dc.subjectfemale
dc.subjectgene
dc.subjectHelicobacter infection
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectintestine metaplasia
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectpromoter region
dc.subjectretinoblastoma 1 gene
dc.subjectsex
dc.subjectstomach carcinoma
dc.subjectadult
dc.subjectaged
dc.subjectcomplication
dc.subjectgenetics
dc.subjectHelicobacter infection
dc.subjectHelicobacter pylori
dc.subjecthost pathogen interaction
dc.subjectintestine
dc.subjectmetaplasia
dc.subjectmicrobiology
dc.subjectmiddle aged
dc.subjectpathology
dc.subjectphysiology
dc.subjectpolymerase chain reaction
dc.subjectpromoter region
dc.subjectstomach tumor
dc.titleAlteration in methylation pattern of retinoblastoma 1 gene promotor region in intestinal metaplasia with or without helicobacter pylori and gastric cancer patients
dc.typeArticle

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