Synergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype

Simple item page
dc.contributor.authorGorgun C.
dc.contributor.authorOzturk S.
dc.contributor.authorGokalp S.
dc.contributor.authorVatansever S.
dc.contributor.authorGurhan S.I.D.
dc.contributor.authorUrkmez A.S.
dc.date.accessioned2024-07-22T08:11:39Z
dc.date.available2024-07-22T08:11:39Z
dc.date.issued2016
dc.description.abstractHypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three dimensional (3D) in vitro culture models. Osteosarcoma stem cells characterized by CD133 surface protein were isolated from osteosarcoma cell line (SaOS-2) by magnetic-activated cell sorting (MACS) technique. Isolated CD133+ and CD133− cells were cultivated under hypoxic (1% O2) and normoxic conditions (21% O2) for 3 days. For the 3D model, bacterial cellulose scaffold was used as the culture substrate. 3D morphologies of cells were examined by scanning electron microscopy (SEM); RT-PCR and immunocytochemistry staining were used to demonstrate conservation of the cancer stem cell phenotype in 3D environment under hypoxic conditions. Cell viability was shown by MTT assay on 3. and 7. culture days. This study is seen as an introduction to develop a 3D hypoxic cancer stem cell based tumor model to study CSC behavior and tumor genesis in vitro. © 2016
dc.identifier.DOI-ID10.1016/j.ijbiomac.2015.12.053
dc.identifier.issn01418130
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15739
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAC133 Antigen
dc.subjectCell Count
dc.subjectCell Hypoxia
dc.subjectCell Line, Tumor
dc.subjectCell Shape
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectImmunomagnetic Separation
dc.subjectNeoplastic Stem Cells
dc.subjectOsteosarcoma
dc.subjectPhenotype
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectStem Cell Niche
dc.subjectCD133 antigen
dc.subjectcellulose
dc.subjectmembrane protein
dc.subjectmolecular scaffold
dc.subjectCD133 antigen
dc.subjectmessenger RNA
dc.subjectArticle
dc.subjectcancer stem cell
dc.subjectcell hypoxia
dc.subjectcell selection
dc.subjectcell separation
dc.subjectcell structure
dc.subjectcell viability
dc.subjectconservation biology
dc.subjectcontrolled study
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectin vitro study
dc.subjectmagnetic activated cell sorting
dc.subjectMTT assay
dc.subjectphenotype
dc.subjectreverse transcription polymerase chain reaction
dc.subjectSaos 2 cell line
dc.subjectscanning electron microscopy
dc.subjectstem cell niche
dc.subjecttumor microenvironment
dc.subjectcancer stem cell
dc.subjectcell count
dc.subjectcell hypoxia
dc.subjectcell shape
dc.subjectgenetics
dc.subjectimmunohistochemistry
dc.subjectimmunomagnetic separation
dc.subjectmetabolism
dc.subjectosteosarcoma
dc.subjectpathology
dc.subjectphenotype
dc.subjectreal time polymerase chain reaction
dc.subjecttumor cell line
dc.titleSynergistic role of three dimensional niche and hypoxia on conservation of cancer stem cell phenotype
dc.typeArticle

Files

Collections

Scopus Koleksiyonu