Profiling of angiogenic cytokines produced by hormone- And drug-refractory prostate cancer cell lines, PC-3 and DU-145 before and after treatment with gossypol

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dc.contributor.authorKaraca B.
dc.contributor.authorKucukzeybek Y.
dc.contributor.authorGorumlu G.
dc.contributor.authorErten C.
dc.contributor.authorGul M.K.
dc.contributor.authorCengiz E.
dc.contributor.authorAtmaca H.
dc.contributor.authorUzunoglu S.
dc.contributor.authorSanli U.A.
dc.contributor.authorKarabulut B.
dc.contributor.authorUslu R.
dc.date.accessioned2024-07-22T08:22:06Z
dc.date.available2024-07-22T08:22:06Z
dc.date.issued2008
dc.description.abstractIn this study, we aimed to investigate the angiogenic cytokine profiles of hormone- and drug- refractory prostate carcinoma cell lines, PC-3 and DU-145. We also studied the effect of gossypol, a natural polyphenolic cotton-seed extract, on the angiogenic cytokine profile of these cell lines. XTT cell proliferation assay was used for the assessment of cytotoxicity. For apoptosis, both histone-DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used. Angiogenic cytokine profiles of supernatants from both cell lines, before and after treatment with gossypol, were investigated using the human angiogenesis antibody array I®. It was shown that the two different hormone- and drug-resistant prostate cancer cell lines, PC-3 and DU-145, constitutively express some important angiogenic cytokines, which are known to regulate tumorigenic- ity and angiogenesis in hormone-refractory prostate cancer. However, PC-3 and DU-145 cells have distinct angiogenic cytokine profiles. In addition, these two cells lines respond differently to gossypol treatment in terms of cytotoxicity and angiogenic cytokine secretion. After treatment with 10 μM of gossypol, there was a 1.5-fold decrease in angiogenin and IL-8 levels and a 1.7- and 1.8-fold decrease in ENA-78 and GRO-α levels respectively, in DU-145 cells. For PC-3 cells, there were 1.6- and 1.8-fold decreases in IL-8 and VEGF levels, respectively. We conclude that PC-3 and DU-145 cells secrete significant amounts of different angiogenic cytokines that may explain their aggressive nature and metastatic potential. Gossypol treatment affects angiogenic cyto- kine secretion from these two cell lines in a different manner. By expanding our knowledge of the heterogeneous biological behavior of these two cell lines, novel treatment approaches can be developed for the treatment of prostate cancer.
dc.identifier.DOI-ID10.1684/ecn.2008.0139
dc.identifier.issn11485493
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18933
dc.language.isoEnglish
dc.subjectAntineoplastic Agents
dc.subjectCaspase 3
dc.subjectCaspase 7
dc.subjectCell Line, Tumor
dc.subjectCytokines
dc.subjectDrug Resistance, Neoplasm
dc.subjectGossypol
dc.subjectHormones
dc.subjectHumans
dc.subjectMale
dc.subjectNeovascularization, Pathologic
dc.subjectProstatic Neoplasms
dc.subjectSubstrate Specificity
dc.subjectBos
dc.subjectGossypium hirsutum
dc.subjectangiogenin
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectcytokine
dc.subjectepithelial derived neutrophil activating factor 78
dc.subjectgossypol
dc.subjectgrowth related gene alpha
dc.subjectinterleukin 8
dc.subjectprotein
dc.subjectunclassified drug
dc.subjectvasculotropin
dc.subjectantineoplastic agent
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectcytokine
dc.subjectgossypol
dc.subjecthormone
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcancer cell culture
dc.subjectcell proliferation
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectcytokine release
dc.subjectcytotoxicity
dc.subjectDNA fragmentation
dc.subjectDNA histone interaction
dc.subjectenzyme activity
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmeasurement
dc.subjectprostate cancer
dc.subjectprotein expression
dc.subjectbiosynthesis
dc.subjectdrug effect
dc.subjectdrug resistance
dc.subjectenzyme specificity
dc.subjectmale
dc.subjectmetabolism
dc.subjectneovascularization (pathology)
dc.subjectpathology
dc.subjectprostate tumor
dc.subjectsecretion
dc.subjecttumor cell line
dc.subjectvascularization
dc.titleProfiling of angiogenic cytokines produced by hormone- And drug-refractory prostate cancer cell lines, PC-3 and DU-145 before and after treatment with gossypol
dc.typeArticle

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