Effect of High dose Gonadotropin Stimulation on Follicular Atresia through Light Chain 3B and Voltage dependent Anion Channel 2

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dc.contributor.authorÖzbilgin M.K.
dc.contributor.authorÖztatlıcı M.
dc.contributor.authorÜçöz M.
dc.date.accessioned2024-07-22T08:03:55Z
dc.date.available2024-07-22T08:03:55Z
dc.date.issued2022
dc.description.abstractBackground: Follicle development takes place under the control of hormonal and environmental stimuli. It suggested that to improve in vitro fertilisation outcomes in poor responders increasing gonadotropin doses be used. Excessive gonadotropin leads to atresia and impairs follicular development, but the molecular mechanisms of follicular atresia remain largely unknown. Recently, it was suggested that autophagy may be an alternative mechanism involved in follicle depletion. Aims: In this study, we aimed to clarify the role of autophagic markers such as light chain (LC) 3B and voltage dependent anion channel 2 (VDAC2) in follicular atresia using the high dose gonadotropin stimulation. Settings and Design: The female 24 BALB/c mice were employed in the present study under the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines with ethical clearance from the institutional ethical committee. These mice were categorised into four groups, with six rats in each as control and test animals. Materials and Methods: Group 1 (control): no action will be taken. Group 2 (sham): only saline will be applied. Group 3: low-dose gonadotropin Pregnant mare's serum gonadotropin (PMSG) + human chorionic gonadotropin (HCG) will be applied. Group 4: high-dose gonadotropin + HCG will be applied. The animals were sacrificed 48 h after the last injection. For all group samples, both protein and mRNAs of the LC3B and VDAC2 were examined by immunohistochemical and reverse transcription-polymerase chain reaction techniques. Statistical Analysis Used: All variables were analysed using GraphPad Prism 8. Kruskal–Wallis t-test and Mann–Whitney U test were used to compare immunohistochemical results; in addition to this, parametric one‐way ANOVA test and Shapiro–Wilk test were applied for quantitative polymerase chain reaction statistics. Results: An increased number of atretic follicles were observed in the high-dose gonadotropin + HCG group. LC3B immunoreactivity of the atretic secondary follicles in the high-dose group is higher than in other groups. The expression of VDAC2 protein in the secondary and Graafian follicles and also VDAC2 mRNA in the ovary were more highly expressed in the control and sham groups. The decrease in VDAC2 mRNA level and immunohistochemical expression was remarkable in the low-dose and high-dose follicle-stimulating hormone groups compared to the control and sham groups. Conclusion: In this study, the increased LC3B and decreased VDAC2 expression, which are autophagy markers, were observed in both the gonadotropins groups, so we suggested that high doses of gonadotropins may cause ovarian atresia. © 2022 Journal of Human Reproductive Sciences | Published by Wolters Kluwer - Medknow.
dc.identifier.DOI-ID10.4103/jhrs.jhrs_143_22
dc.identifier.issn09741208
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12478
dc.language.isoEnglish
dc.publisherWolters Kluwer Medknow Publications
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectbiological marker
dc.subjectchorionic gonadotropin
dc.subjectgonadotropin
dc.subjectlight chain 3b protein
dc.subjectmessenger RNA
dc.subjectunclassified drug
dc.subjectvoltage dependent anion channel 2
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectautophagy (cellular)
dc.subjectcontrolled study
dc.subjectdose response
dc.subjectdrug dose comparison
dc.subjectdrug megadose
dc.subjectfemale
dc.subjectgene expression
dc.subjectgonadotropin blood level
dc.subjectgranulosa cell
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectlow drug dose
dc.subjectmouse
dc.subjectnonhuman
dc.subjectovary follicle
dc.subjectovary follicle atresia
dc.subjectovary tissue
dc.subjectprimordial follicle
dc.subjectreal time polymerase chain reaction
dc.subjectreverse transcription polymerase chain reaction
dc.titleEffect of High dose Gonadotropin Stimulation on Follicular Atresia through Light Chain 3B and Voltage dependent Anion Channel 2
dc.typeArticle

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